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Literature summary extracted from

  • Fujioka, M.
    Purification and properties of serine hydroxymethylase from soluble and mitochondrial fractions of rabbit liver (1969), Biochim. Biophys. Acta, 185, 338-349.
    View publication on PubMed

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.1.2.1 mitochondrial enzyme Oryctolagus cuniculus

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.1.2.1 1,10-phenanthroline
-
 Oryctolagus cuniculus
2.1.2.1 DL-2-methylserine
-
 Oryctolagus cuniculus
2.1.2.1 EDTA weak Oryctolagus cuniculus
2.1.2.1 hydrazine
-
 Oryctolagus cuniculus
2.1.2.1 additional information not: iodoacetate; not: NaN3, mono- or divalent cations, 2-mercaptoethanol, DTT; not: purine nucleoside mono-, di- and triphosphates Oryctolagus cuniculus
2.1.2.1 N-ethylmaleimide
-
 Oryctolagus cuniculus
2.1.2.1 NH2OH weak Oryctolagus cuniculus
2.1.2.1 PCMB
-
 Oryctolagus cuniculus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.1.2.1 1
-
 L-serine mitochondrial enzyme Oryctolagus cuniculus
2.1.2.1 1.3
-
 L-serine cytosolic enzyme Oryctolagus cuniculus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.1.2.1 cytoplasm
-
 Oryctolagus cuniculus 5737
-
2.1.2.1 mitochondrion
-
 Oryctolagus cuniculus 5739
-
2.1.2.1 additional information isozymes in cytosol and mitochondria Oryctolagus cuniculus
-
-
2.1.2.1 additional information immunochemically different Oryctolagus cuniculus
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.1.2.1 Ca2+ not Oryctolagus cuniculus
2.1.2.1 Mg2+ not Oryctolagus cuniculus
2.1.2.1 Mn2+ not Oryctolagus cuniculus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.1.2.1 170000
-
 mitochondria, sedimentation equilibrium centrifugation Oryctolagus cuniculus
2.1.2.1 185000
-
 soluble, high speed sedimentation equilibrium centrifugation Oryctolagus cuniculus

Organism

EC Number Organism UniProt Comment Textmining
2.1.2.1 Oryctolagus cuniculus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.1.2.1
-
 Oryctolagus cuniculus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.1.2.1 additional information
-
-
 Oryctolagus cuniculus
2.1.2.1 10
-
 soluble Oryctolagus cuniculus
2.1.2.1 15.5
-
 mitochondria Oryctolagus cuniculus

Storage Stability

EC Number Storage Stability Organism
2.1.2.1 5°C, precipitation after several days Oryctolagus cuniculus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.2.1 alpha-methylserine + tetrahydrofolate at high concentrations of enzyme Oryctolagus cuniculus D-alanine + 5,10-methylenetetrahydrofolate
-
 ?
2.1.2.1 L-serine + tetrahydrofolate
-
 Oryctolagus cuniculus glycine + 5,10-methylenetetrahydrofolate + H2O
-
 r

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.1.2.1 7.3
-
-
 Oryctolagus cuniculus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.1.2.1 6 8.5 cytosol: about 60% of maximal activity at pH 6, about 70% at 8.5, mitochondria: about 40% of maximal activity at pH 6.0 and 8.5 Oryctolagus cuniculus

Cofactor

EC Number Cofactor Comment Organism Structure
2.1.2.1 pyridoxal 5'-phosphate requirement, 4 mol per mol enzyme Oryctolagus cuniculus

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.1.2.1 3.9
-
 DL-2-methylserine soluble fraction Oryctolagus cuniculus
2.1.2.1 11.2
-
 DL-2-methylserine mitochondrial enzyme Oryctolagus cuniculus