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Literature summary extracted from

  • Alston, T.A.; Abeles, R.H.
    Substrate specificity of nicotinamide methyltransferase isolated from porcine liver (1988), Arch. Biochem. Biophys., 260, 601-608.
    View publication on PubMed

General Stability

EC Number General Stability Organism
2.1.1.1 glycerol stabilizes Sus scrofa
2.1.1.1 thiols required for stability during isolation Sus scrofa

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.1.1.1 0.016
-
 S-adenosyl-L-methionine
-
 Sus scrofa
2.1.1.1 0.2
-
 1,2,3,4-tetrahydroisoquinoline
-
 Sus scrofa
2.1.1.1 0.83
-
 nicotinamide
-
 Sus scrofa
2.1.1.1 1.3
-
 Quinoline
-
 Sus scrofa
2.1.1.1 1.5
-
 Isoquinoline
-
 Sus scrofa

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.1.1.1 27000
-
 1 * 27000, SDS-PAGE Sus scrofa
2.1.1.1 27000 30000 gel filtration, SDS-PAGE Sus scrofa

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.1.1.1 additional information Sus scrofa enzyme may function in detoxificating of numerous alkaloids ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.1.1.1 Sus scrofa
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.1.1.1
-
 Sus scrofa

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.1.1.1 liver
-
 Sus scrofa
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.1.1.1 additional information
-
 assay Sus scrofa

Storage Stability

EC Number Storage Stability Organism
2.1.1.1 -20°C, 20% glycerol w/v, stable for 1 month, partially purified enzyme Sus scrofa
2.1.1.1 4°C, 20% glycerol w/v, pH 7.6, 30% loss of activity, purified enzyme Sus scrofa
2.1.1.1 4°C, 20% w/v glycerol, stable for 1 week, partially purified enzyme Sus scrofa

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.1.1 additional information enzyme may function in detoxificating of numerous alkaloids Sus scrofa ?
-
 ?
2.1.1.1 S-adenosyl-L-methionine + 1,2,3,4-tetrahydroisoquinoline
-
 Sus scrofa S-adenosyl-L-homocysteine + ?
-
 ?
2.1.1.1 S-adenosyl-L-methionine + 3-acetylpyridine
-
 Sus scrofa S-adenosyl-L-homocysteine + ?
-
 ?
2.1.1.1 S-adenosyl-L-methionine + isoquinoline
-
 Sus scrofa S-adenosyl-L-homocysteine + ?
-
 ?
2.1.1.1 S-adenosyl-L-methionine + nicotinamide
-
 Sus scrofa S-adenosyl-L-homocysteine + 1-methylnicotinamide
-
 ?
2.1.1.1 S-adenosyl-L-methionine + quinoline
-
 Sus scrofa S-adenosyl-L-homocysteine + ?
-
 ?
2.1.1.1 S-adenosyl-L-methionine + thionicotinamide
-
 Sus scrofa S-adenosyl-L-homocysteine + 1-methylthionicotinamide
-
 ?

Subunits

EC Number Subunits Comment Organism
2.1.1.1 monomer 1 * 27000, SDS-PAGE Sus scrofa

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.1.1.1 25
-
 assay at Sus scrofa

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.1.1.1 7.2
-
-
 Sus scrofa

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.1.1.1 5.7 9.8 about 50% of maximum activity at pH 5.7 and 9.8 Sus scrofa