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Literature summary extracted from

  • Lee, K.H.; Cava, M.; Amiri, P.; Ottoboni, T.; Lindquist, R.N.
    Betaine:homocysteine methyltransferase from rat liver: purification and inhibition by a boronic acid substrate analog (1992), Arch. Biochem. Biophys., 292, 77-86.
    View publication on PubMed

General Stability

EC Number General Stability Organism
2.1.1.5 glycerol does not stabilize Rattus norvegicus

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.1.1.5 Pinanyl N,N,N-trimethylaminomethane boronate substrate analogue Rattus norvegicus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.1.1.5 0.12
-
 L-homocysteine betaine Rattus norvegicus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.1.1.5 45000
-
 6 * 45000, SDS-PAGE Rattus norvegicus
2.1.1.5 270000
-
 gel filtration Rattus norvegicus

Organism

EC Number Organism UniProt Comment Textmining
2.1.1.5 Rattus norvegicus
-
-
-
2.1.1.5 Rattus norvegicus Sprague-Dawley
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.1.1.5 FPLC-chromatofocusing, 3 active forms Rattus norvegicus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.1.1.5 liver
-
 Rattus norvegicus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.1.1.5 0.0187
-
-
 Rattus norvegicus

Storage Stability

EC Number Storage Stability Organism
2.1.1.5 -20°C, 2 weeks in the presence of substrates Rattus norvegicus
2.1.1.5 -20°C, 40% loss of activity after 4 weeks in the presence of substrates Rattus norvegicus
2.1.1.5 -20°C, 70% loss of activity after 10 weeks in the presence of substrates Rattus norvegicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.1.5 N,N,N-trimethylglycine + L-homocysteine
-
 Rattus norvegicus N,N-dimethylglycine + L-methionine
-
 ?
2.1.1.5 N,N,N-trimethylglycine + L-homocysteine
-
 Rattus norvegicus Sprague-Dawley N,N-dimethylglycine + L-methionine
-
 ?

Subunits

EC Number Subunits Comment Organism
2.1.1.5 hexamer 6 * 45000, SDS-PAGE Rattus norvegicus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.1.1.5 additional information
-
 3 active forms with pI: 7.0, 7.6 and 8.0 Rattus norvegicus

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.1.1.5 0.045
-
 Pinanyl N,N,N-trimethylaminomethane boronate
-
 Rattus norvegicus