BRENDA - Enzyme Database

Characterization of deoxycytidylate methyltransferase in Xanthomonas oryzae infected with bacteriophage Xp12

Feng, T.Y.; Tu, J.; Kuo, T.T.; Eur. J. Biochem. 87, 29-36 (1978)

Data extracted from this reference:

Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
2.1.1.54
Cu2+
above 1 mM
Xanthomonas oryzae
2.1.1.54
Mg2+
above 50 mM
Xanthomonas oryzae
2.1.1.54
Zn2+
above 1 mM
Xanthomonas oryzae
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.1.1.54
0.0313
-
dCMP
-
Xanthomonas oryzae
2.1.1.54
0.0714
-
tetrahydrofolic acid
-
Xanthomonas oryzae
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
2.1.1.54
Ca2+
25 mM, slight stimulation
Xanthomonas oryzae
2.1.1.54
Cu2+
below 1 mM, stimulation
Xanthomonas oryzae
2.1.1.54
K+
150 mM, stimulation
Xanthomonas oryzae
2.1.1.54
Mg2+
stimulates below 25 mM
Xanthomonas oryzae
2.1.1.54
Mn2+
has a similar effect in stimulation but not as strong as Mg2+
Xanthomonas oryzae
2.1.1.54
additional information
among the buffers tested, 6-morpholinoethane sulfonate/Mg2+ is the best
Xanthomonas oryzae
2.1.1.54
Zn2+
below 1 mM, stimulation
Xanthomonas oryzae
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
2.1.1.54
Xanthomonas oryzae
-
phage Xp12-infected
-
Purification (Commentary)
EC Number
Commentary
Organism
2.1.1.54
phage Xp12-infected, partial
Xanthomonas oryzae
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [mol/min/mg]
Specific Activity Maximum [mol/min/mg]
Commentary
Organism
2.1.1.54
0.11
-
-
Xanthomonas oryzae
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.1.1.54
5,10-methylenetetrahydrofolate + CMP
-
441229
Xanthomonas oryzae
dihydrofolate + 5-methylcytidylate
-
-
-
?
2.1.1.54
5,10-methylenetetrahydrofolate + CTP
low activity
441229
Xanthomonas oryzae
dihydrofolate + 5-methylcytidine 5'-triphosphate
-
-
-
-
2.1.1.54
5,10-methylenetetrahydrofolate + dCMP
-
441229
Xanthomonas oryzae
dihydrofolate + deoxy-5-methylcytidylate
-
-
-
-
2.1.1.54
5,10-methylenetetrahydrofolate + dCTP
low activity
441229
Xanthomonas oryzae
dihydrofolate + deoxy-5-methylcytidine 5'-triphosphate
-
-
-
?
Temperature Optimum [C]
EC Number
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
2.1.1.54
30
-
assay at
Xanthomonas oryzae
Temperature Stability [C]
EC Number
Temperature Stability Minimum [C]
Temperature Stability Maximum [C]
Commentary
Organism
2.1.1.54
50
-
3 min, 90% loss of activity
Xanthomonas oryzae
2.1.1.54
70
-
3 min, complete loss of activity
Xanthomonas oryzae
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.1.1.54
6
-
-
Xanthomonas oryzae
pH Range
EC Number
pH Minimum
pH Maximum
Commentary
Organism
2.1.1.54
additional information
-
-
Xanthomonas oryzae
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
2.1.1.54
Cu2+
above 1 mM
Xanthomonas oryzae
2.1.1.54
Mg2+
above 50 mM
Xanthomonas oryzae
2.1.1.54
Zn2+
above 1 mM
Xanthomonas oryzae
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.1.1.54
0.0313
-
dCMP
-
Xanthomonas oryzae
2.1.1.54
0.0714
-
tetrahydrofolic acid
-
Xanthomonas oryzae
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
2.1.1.54
Ca2+
25 mM, slight stimulation
Xanthomonas oryzae
2.1.1.54
Cu2+
below 1 mM, stimulation
Xanthomonas oryzae
2.1.1.54
K+
150 mM, stimulation
Xanthomonas oryzae
2.1.1.54
Mg2+
stimulates below 25 mM
Xanthomonas oryzae
2.1.1.54
Mn2+
has a similar effect in stimulation but not as strong as Mg2+
Xanthomonas oryzae
2.1.1.54
additional information
among the buffers tested, 6-morpholinoethane sulfonate/Mg2+ is the best
Xanthomonas oryzae
2.1.1.54
Zn2+
below 1 mM, stimulation
Xanthomonas oryzae
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
2.1.1.54
phage Xp12-infected, partial
Xanthomonas oryzae
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [mol/min/mg]
Specific Activity Maximum [mol/min/mg]
Commentary
Organism
2.1.1.54
0.11
-
-
Xanthomonas oryzae
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.1.1.54
5,10-methylenetetrahydrofolate + CMP
-
441229
Xanthomonas oryzae
dihydrofolate + 5-methylcytidylate
-
-
-
?
2.1.1.54
5,10-methylenetetrahydrofolate + CTP
low activity
441229
Xanthomonas oryzae
dihydrofolate + 5-methylcytidine 5'-triphosphate
-
-
-
-
2.1.1.54
5,10-methylenetetrahydrofolate + dCMP
-
441229
Xanthomonas oryzae
dihydrofolate + deoxy-5-methylcytidylate
-
-
-
-
2.1.1.54
5,10-methylenetetrahydrofolate + dCTP
low activity
441229
Xanthomonas oryzae
dihydrofolate + deoxy-5-methylcytidine 5'-triphosphate
-
-
-
?
Temperature Optimum [C] (protein specific)
EC Number
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
2.1.1.54
30
-
assay at
Xanthomonas oryzae
Temperature Stability [C] (protein specific)
EC Number
Temperature Stability Minimum [C]
Temperature Stability Maximum [C]
Commentary
Organism
2.1.1.54
50
-
3 min, 90% loss of activity
Xanthomonas oryzae
2.1.1.54
70
-
3 min, complete loss of activity
Xanthomonas oryzae
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.1.1.54
6
-
-
Xanthomonas oryzae
pH Range (protein specific)
EC Number
pH Minimum
pH Maximum
Commentary
Organism
2.1.1.54
additional information
-
-
Xanthomonas oryzae