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Literature summary extracted from

  • Upmeier, B.; Gross, W.; Köster, S.; Barz, W.
    Purification and properties of S-adenosyl-L-methionine:nicotinic acid-N-methyltransferase from cell suspension cultures of Glycine max L (1988), Arch. Biochem. Biophys., 262, 445-454.
    View publication on PubMed

General Stability

EC Number General Stability Organism
2.1.1.7 no stabilization by glycerol, 10-40% v/v, or nicotinic acid Glycine max
2.1.1.7 sulfhydryl group protecting agents required for stabilization during purification Glycine max
2.1.1.7 total loss of activity by freezing at -20°C Glycine max

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.1.1.7 Hg2+
-
Glycine max
2.1.1.7 Mg2+ slight Glycine max
2.1.1.7 p-hydroxymercuribenzoate
-
Glycine max
2.1.1.7 S-adenosyl-L-homocysteine
-
Glycine max
2.1.1.7 SH-blocking reagents
-
Glycine max

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.1.1.7 0.055
-
S-adenosyl-L-methionine
-
Glycine max
2.1.1.7 0.078
-
nicotinate
-
Glycine max

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.1.1.7 additional information no requirement for divalent cations Glycine max

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.1.1.7 90000
-
ultracentrifugation, gel filtration Glycine max

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.1.1.7 S-adenosyl-L-methionine + nicotinate Glycine max
-
S-adenosyl-L-homocysteine + 1-methylnicotinate
-
?

Organism

EC Number Organism UniProt Comment Textmining
2.1.1.7 Glycine max
-
soybean
-

Oxidation Stability

EC Number Oxidation Stability Organism
2.1.1.7 rigorous exclusion of O2 by saturating the buffer with N2 allows partial stabilization during purification Glycine max

Purification (Commentary)

EC Number Purification (Comment) Organism
2.1.1.7
-
Glycine max

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.1.1.7 cell suspension culture heterotrophic Glycine max
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.1.1.7 additional information
-
-
Glycine max

Storage Stability

EC Number Storage Stability Organism
2.1.1.7 4°C, 24 h, N2-atmosphere Glycine max

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.1.7 S-adenosyl-L-methionine + nicotinate
-
Glycine max S-adenosyl-L-homocysteine + 1-methylnicotinate
-
?
2.1.1.7 S-adenosyl-L-methionine + nicotinate high specificity for nicotinate, no other pyridine derivative is a suitable methyl group acceptor Glycine max S-adenosyl-L-homocysteine + 1-methylnicotinate
-
?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.1.1.7 35 40
-
Glycine max

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.1.1.7 8
-
-
Glycine max

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.1.1.7 5 9 pH 5.0: about 50% of maximum activity, pH 9.0: nearly inactive Glycine max