EC Number | General Stability | Organism |
---|---|---|
2.1.1.7 | no stabilization by glycerol, 10-40% v/v, or nicotinic acid | Glycine max |
2.1.1.7 | sulfhydryl group protecting agents required for stabilization during purification | Glycine max |
2.1.1.7 | total loss of activity by freezing at -20°C | Glycine max |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
2.1.1.7 | Hg2+ | - |
Glycine max | |
2.1.1.7 | Mg2+ | slight | Glycine max | |
2.1.1.7 | p-hydroxymercuribenzoate | - |
Glycine max | |
2.1.1.7 | S-adenosyl-L-homocysteine | - |
Glycine max | |
2.1.1.7 | SH-blocking reagents | - |
Glycine max |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.1.1.7 | 0.055 | - |
S-adenosyl-L-methionine | - |
Glycine max | |
2.1.1.7 | 0.078 | - |
nicotinate | - |
Glycine max |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
2.1.1.7 | additional information | no requirement for divalent cations | Glycine max |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
2.1.1.7 | 90000 | - |
ultracentrifugation, gel filtration | Glycine max |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.1.1.7 | S-adenosyl-L-methionine + nicotinate | Glycine max | - |
S-adenosyl-L-homocysteine + 1-methylnicotinate | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.1.1.7 | Glycine max | - |
soybean | - |
EC Number | Oxidation Stability | Organism |
---|---|---|
2.1.1.7 | rigorous exclusion of O2 by saturating the buffer with N2 allows partial stabilization during purification | Glycine max |
EC Number | Purification (Comment) | Organism |
---|---|---|
2.1.1.7 | - |
Glycine max |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
2.1.1.7 | cell suspension culture | heterotrophic | Glycine max | - |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
2.1.1.7 | additional information | - |
- |
Glycine max |
EC Number | Storage Stability | Organism |
---|---|---|
2.1.1.7 | 4°C, 24 h, N2-atmosphere | Glycine max |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.1.1.7 | S-adenosyl-L-methionine + nicotinate | - |
Glycine max | S-adenosyl-L-homocysteine + 1-methylnicotinate | - |
? | |
2.1.1.7 | S-adenosyl-L-methionine + nicotinate | high specificity for nicotinate, no other pyridine derivative is a suitable methyl group acceptor | Glycine max | S-adenosyl-L-homocysteine + 1-methylnicotinate | - |
? |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
2.1.1.7 | 35 | 40 | - |
Glycine max |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
2.1.1.7 | 8 | - |
- |
Glycine max |
EC Number | pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|---|
2.1.1.7 | 5 | 9 | pH 5.0: about 50% of maximum activity, pH 9.0: nearly inactive | Glycine max |