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Literature summary extracted from

  • Taylor, R.T.; Weissbach, H.
    N5-Methyltetrahydrofolate-homocysteine methyltransferases (1973), The Enzymes, 3rd Ed. (Boyer, P. D. , ed. ), 12, 121-165.
No PubMed abstract available

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.1.1.13 additional information
-
 additional information
-
 Escherichia coli
2.1.1.13 0.0016
-
 S-adenosyl-L-methionine
-
 Escherichia coli
2.1.1.13 0.016
-
 L-homocysteine
-
 Escherichia coli
2.1.1.13 0.03
-
 5-methyltetrahydrofolate
-
 Escherichia coli

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.1.1.13 cytoplasm
-
 Rattus norvegicus 5737
-
2.1.1.13 mitochondrion
-
 Rattus norvegicus 5739
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.1.1.13 140000 150000 gel filtration, sedimentation coefficient in sucrose density gradients Escherichia coli

Organism

EC Number Organism UniProt Comment Textmining
2.1.1.13 Auxenochlorella pyrenoidosa
-
-
-
2.1.1.13 Bos taurus
-
-
-
2.1.1.13 Cereibacter sphaeroides
-
-
-
2.1.1.13 Escherichia coli
-
-
-
2.1.1.13 Gallus gallus
-
-
-
2.1.1.13 Homo sapiens
-
-
-
2.1.1.13 Klebsiella aerogenes
-
-
-
2.1.1.13 Ochromonas malhamensis
-
-
-
2.1.1.13 Rattus norvegicus
-
-
-
2.1.1.13 Salmonella enterica subsp. enterica serovar Typhimurium
-
-
-
2.1.1.13 Sus scrofa
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.1.1.13 B Escherichia coli

Reaction

EC Number Reaction Comment Organism Reaction ID
2.1.1.13 5-methyltetrahydrofolate + L-homocysteine = tetrahydrofolate + L-methionine mechanism Salmonella enterica subsp. enterica serovar Typhimurium
a
2.1.1.13 5-methyltetrahydrofolate + L-homocysteine = tetrahydrofolate + L-methionine mechanism Gallus gallus
a
2.1.1.13 5-methyltetrahydrofolate + L-homocysteine = tetrahydrofolate + L-methionine mechanism Escherichia coli
a
2.1.1.13 5-methyltetrahydrofolate + L-homocysteine = tetrahydrofolate + L-methionine mechanism Homo sapiens
a
2.1.1.13 5-methyltetrahydrofolate + L-homocysteine = tetrahydrofolate + L-methionine mechanism Rattus norvegicus
a
2.1.1.13 5-methyltetrahydrofolate + L-homocysteine = tetrahydrofolate + L-methionine mechanism Sus scrofa
a
2.1.1.13 5-methyltetrahydrofolate + L-homocysteine = tetrahydrofolate + L-methionine mechanism Bos taurus
a
2.1.1.13 5-methyltetrahydrofolate + L-homocysteine = tetrahydrofolate + L-methionine mechanism Cereibacter sphaeroides
a
2.1.1.13 5-methyltetrahydrofolate + L-homocysteine = tetrahydrofolate + L-methionine mechanism Klebsiella aerogenes
a
2.1.1.13 5-methyltetrahydrofolate + L-homocysteine = tetrahydrofolate + L-methionine mechanism Auxenochlorella pyrenoidosa
a
2.1.1.13 5-methyltetrahydrofolate + L-homocysteine = tetrahydrofolate + L-methionine mechanism Ochromonas malhamensis
a

Renatured (Commentary)

EC Number Renatured (Comment) Organism
2.1.1.13 incubation of resolved apoenzyme with methyl-B12 results in spontaneous formation of holoenzyme Escherichia coli

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.1.1.13 brain
-
 Homo sapiens
-
2.1.1.13 brain
-
 Bos taurus
-
2.1.1.13 kidney
-
 Homo sapiens
-
2.1.1.13 kidney
-
 Sus scrofa
-
2.1.1.13 kidney
-
 Bos taurus
-
2.1.1.13 liver
-
 Gallus gallus
-
2.1.1.13 liver
-
 Homo sapiens
-
2.1.1.13 liver
-
 Rattus norvegicus
-
2.1.1.13 liver
-
 Sus scrofa
-
2.1.1.13 liver
-
 Bos taurus
-
2.1.1.13 tissue culture
-
 Homo sapiens
-
2.1.1.13 tissue culture a variety of mammalian cell lines in tissue culture Rattus norvegicus
-
2.1.1.13 tissue culture a variety of mammalian cell lines in tissue culture Sus scrofa
-
2.1.1.13 tissue culture a variety of mammalian cell lines in tissue culture Bos taurus
-
2.1.1.13 tissue culture a variety of mammalian cell lines in tissue culture Auxenochlorella pyrenoidosa
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.1.1.13 additional information
-
-
 Salmonella enterica subsp. enterica serovar Typhimurium
2.1.1.13 additional information
-
-
 Gallus gallus
2.1.1.13 additional information
-
-
 Rattus norvegicus
2.1.1.13 additional information
-
-
 Bos taurus
2.1.1.13 additional information
-
-
 Cereibacter sphaeroides
2.1.1.13 additional information
-
-
 Klebsiella aerogenes
2.1.1.13 additional information
-
-
 Auxenochlorella pyrenoidosa
2.1.1.13 additional information
-
-
 Ochromonas malhamensis
2.1.1.13 additional information
-
 assay method Escherichia coli
2.1.1.13 additional information
-
 assay method Homo sapiens
2.1.1.13 additional information
-
 assay method Sus scrofa

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.1.13 5-methyltetrahydrofolate + 2-mercaptoethanol
-
 Escherichia coli S-methylmercaptoethanol + tetrahydrofolate
-
 ?
2.1.1.13 5-methyltetrahydrofolate + L-homocysteine
-
 Escherichia coli tetrahydrofolate + L-methionine
-
 ?
2.1.1.13 N5-methyltetrahydropteroylmonoglutamate + L-homocysteine
-
 Salmonella enterica subsp. enterica serovar Typhimurium tetrahydropteroylmonoglutamate + L-methionine
-
 ?
2.1.1.13 N5-methyltetrahydropteroylmonoglutamate + L-homocysteine
-
 Gallus gallus tetrahydropteroylmonoglutamate + L-methionine
-
 ?
2.1.1.13 N5-methyltetrahydropteroylmonoglutamate + L-homocysteine
-
 Escherichia coli tetrahydropteroylmonoglutamate + L-methionine
-
 ?
2.1.1.13 N5-methyltetrahydropteroylmonoglutamate + L-homocysteine
-
 Homo sapiens tetrahydropteroylmonoglutamate + L-methionine
-
 ?
2.1.1.13 N5-methyltetrahydropteroylmonoglutamate + L-homocysteine
-
 Rattus norvegicus tetrahydropteroylmonoglutamate + L-methionine
-
 ?
2.1.1.13 N5-methyltetrahydropteroylmonoglutamate + L-homocysteine
-
 Sus scrofa tetrahydropteroylmonoglutamate + L-methionine
-
 ?
2.1.1.13 N5-methyltetrahydropteroylmonoglutamate + L-homocysteine
-
 Bos taurus tetrahydropteroylmonoglutamate + L-methionine
-
 ?
2.1.1.13 N5-methyltetrahydropteroylmonoglutamate + L-homocysteine
-
 Cereibacter sphaeroides tetrahydropteroylmonoglutamate + L-methionine
-
 ?
2.1.1.13 N5-methyltetrahydropteroylmonoglutamate + L-homocysteine
-
 Klebsiella aerogenes tetrahydropteroylmonoglutamate + L-methionine
-
 ?
2.1.1.13 N5-methyltetrahydropteroylmonoglutamate + L-homocysteine
-
 Auxenochlorella pyrenoidosa tetrahydropteroylmonoglutamate + L-methionine
-
 ?
2.1.1.13 N5-methyltetrahydropteroylmonoglutamate + L-homocysteine
-
 Ochromonas malhamensis tetrahydropteroylmonoglutamate + L-methionine
-
 ?
2.1.1.13 N5-methyltetrahydropteroyltriglutamate + L-homocysteine
-
 Salmonella enterica subsp. enterica serovar Typhimurium tetrahydropteroyltriglutamate + L-methionine
-
 ?
2.1.1.13 N5-methyltetrahydropteroyltriglutamate + L-homocysteine
-
 Gallus gallus tetrahydropteroyltriglutamate + L-methionine
-
 ?
2.1.1.13 N5-methyltetrahydropteroyltriglutamate + L-homocysteine
-
 Escherichia coli tetrahydropteroyltriglutamate + L-methionine
-
 ?
2.1.1.13 N5-methyltetrahydropteroyltriglutamate + L-homocysteine
-
 Homo sapiens tetrahydropteroyltriglutamate + L-methionine
-
 ?
2.1.1.13 N5-methyltetrahydropteroyltriglutamate + L-homocysteine
-
 Rattus norvegicus tetrahydropteroyltriglutamate + L-methionine
-
 ?
2.1.1.13 N5-methyltetrahydropteroyltriglutamate + L-homocysteine
-
 Sus scrofa tetrahydropteroyltriglutamate + L-methionine
-
 ?
2.1.1.13 N5-methyltetrahydropteroyltriglutamate + L-homocysteine
-
 Bos taurus tetrahydropteroyltriglutamate + L-methionine
-
 ?
2.1.1.13 N5-methyltetrahydropteroyltriglutamate + L-homocysteine
-
 Cereibacter sphaeroides tetrahydropteroyltriglutamate + L-methionine
-
 ?
2.1.1.13 N5-methyltetrahydropteroyltriglutamate + L-homocysteine
-
 Klebsiella aerogenes tetrahydropteroyltriglutamate + L-methionine
-
 ?
2.1.1.13 N5-methyltetrahydropteroyltriglutamate + L-homocysteine
-
 Auxenochlorella pyrenoidosa tetrahydropteroyltriglutamate + L-methionine
-
 ?
2.1.1.13 N5-methyltetrahydropteroyltriglutamate + L-homocysteine
-
 Ochromonas malhamensis tetrahydropteroyltriglutamate + L-methionine
-
 ?
2.1.1.13 S-adenosyl-L-methionine + L-homocysteine
-
 Escherichia coli methionine + S-adenosyl-L-homocysteine
-
 ?
2.1.1.13 S-adenosyl-L-methionine + tetrahydrofolate
-
 Escherichia coli 5-methyltetrahydrofolate + S-adenosyl-L-homocysteine
-
 ?

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.1.1.13 0.0217
-
 S-adenosyl-L-methionine
-
 Escherichia coli
2.1.1.13 0.0367
-
 S-adenosyl-L-methionine
-
 Escherichia coli
2.1.1.13 8.33 13 5-methyltetrahydrofolate
-
 Escherichia coli

Cofactor

EC Number Cofactor Comment Organism Structure
2.1.1.13 additional information absolute requirement for a reducing system Salmonella enterica subsp. enterica serovar Typhimurium
2.1.1.13 additional information absolute requirement for a reducing system Gallus gallus
2.1.1.13 additional information absolute requirement for a reducing system Escherichia coli
2.1.1.13 additional information absolute requirement for a reducing system Homo sapiens
2.1.1.13 additional information absolute requirement for a reducing system Rattus norvegicus
2.1.1.13 additional information absolute requirement for a reducing system Sus scrofa
2.1.1.13 additional information absolute requirement for a reducing system Bos taurus
2.1.1.13 additional information absolute requirement for a reducing system Cereibacter sphaeroides
2.1.1.13 additional information absolute requirement for a reducing system Klebsiella aerogenes
2.1.1.13 additional information absolute requirement for a reducing system Auxenochlorella pyrenoidosa
2.1.1.13 additional information absolute requirement for a reducing system Ochromonas malhamensis
2.1.1.13 S-adenosyl-L-methionine required Salmonella enterica subsp. enterica serovar Typhimurium
2.1.1.13 S-adenosyl-L-methionine required Gallus gallus
2.1.1.13 S-adenosyl-L-methionine required Escherichia coli
2.1.1.13 S-adenosyl-L-methionine required Homo sapiens
2.1.1.13 S-adenosyl-L-methionine required Rattus norvegicus
2.1.1.13 S-adenosyl-L-methionine required Sus scrofa
2.1.1.13 S-adenosyl-L-methionine required Bos taurus
2.1.1.13 S-adenosyl-L-methionine required Cereibacter sphaeroides
2.1.1.13 S-adenosyl-L-methionine required Klebsiella aerogenes
2.1.1.13 S-adenosyl-L-methionine required Auxenochlorella pyrenoidosa
2.1.1.13 S-adenosyl-L-methionine required Ochromonas malhamensis
2.1.1.13 S-adenosyl-L-methionine role of S-adenosyl-L-methionine Escherichia coli
2.1.1.13 vitamin B12 enzyme contains a derivative of vitamin B12 as prosthetic group Salmonella enterica subsp. enterica serovar Typhimurium
2.1.1.13 vitamin B12 enzyme contains a derivative of vitamin B12 as prosthetic group Gallus gallus
2.1.1.13 vitamin B12 enzyme contains a derivative of vitamin B12 as prosthetic group Escherichia coli
2.1.1.13 vitamin B12 enzyme contains a derivative of vitamin B12 as prosthetic group Homo sapiens
2.1.1.13 vitamin B12 enzyme contains a derivative of vitamin B12 as prosthetic group Rattus norvegicus
2.1.1.13 vitamin B12 enzyme contains a derivative of vitamin B12 as prosthetic group Sus scrofa
2.1.1.13 vitamin B12 enzyme contains a derivative of vitamin B12 as prosthetic group Bos taurus
2.1.1.13 vitamin B12 enzyme contains a derivative of vitamin B12 as prosthetic group Cereibacter sphaeroides
2.1.1.13 vitamin B12 enzyme contains a derivative of vitamin B12 as prosthetic group Klebsiella aerogenes
2.1.1.13 vitamin B12 enzyme contains a derivative of vitamin B12 as prosthetic group Auxenochlorella pyrenoidosa
2.1.1.13 vitamin B12 enzyme contains a derivative of vitamin B12 as prosthetic group Ochromonas malhamensis