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Literature summary extracted from

  • Seki-Chiba, S.; Ishimoto, M.
    Studies on nitrate reductase of Clostridium perfringens. Purification, some properties, and effect of tungstate on its formation (1977), J. Biochem., 82, 1663-1671.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.7.7.2 additional information enzyme synthesis is induced by nitrate Clostridium perfringens

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.7.7.2 azide 74% inhibition Clostridium perfringens
1.7.7.2 cyanide 10 mM, 87% inhibition Clostridium perfringens
1.7.7.2 additional information not inhibited by 0.1 mM p-chloromercuribenzoate, enzyme formation is induced by nitrate and inhibited by 0.5 mM tungstate, but recovered by 0.1 mM molybdate Clostridium perfringens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.7.7.2 0.1
-
 nitrate
-
 Clostridium perfringens

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.7.7.2 cytoplasm enzyme functions in the soluble state in the cytoplasm Clostridium perfringens 5737
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.7.7.2 Ca2+ stimulates activity about 30% Clostridium perfringens
1.7.7.2 Fe2+ stimulates activity about 50% Clostridium perfringens
1.7.7.2 Iron molybdenum iron-sulfur protein Clostridium perfringens
1.7.7.2 iron-sulfur centre molybdo-iron-sulfur protein Clostridium perfringens
1.7.7.2 Mg2+ stimulates activity about 30% Clostridium perfringens
1.7.7.2 Mn2+ stimulates activity about 50%, stimulates reaction rate and level of nitrite formation Clostridium perfringens
1.7.7.2 Molybdenum molybdenum iron-sulfur protein Clostridium perfringens

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.7.7.2 80000
-
 gel filtration Clostridium perfringens
1.7.7.2 90000
-
 ultracentrifugation Clostridium perfringens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.7.7.2 nitrate + reduced ferredoxin Clostridium perfringens nitrate fermentation, ferredoxin is utilized as direct electron donor in nitrate reduction nitrite + oxidized ferredoxin
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.7.7.2 Clostridium perfringens
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.7.7.2
-
 Clostridium perfringens

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.7.7.2 52
-
-
 Clostridium perfringens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.7.7.2 additional information not as electron donors: NADH, NADPH, FADH2, FMNH2, rubredoxin Clostridium perfringens ?
-
 ?
1.7.7.2 nitrate + reduced ferredoxin ferredoxin as electron donor at neutral pH and pH 8, but not at pH 9 Clostridium perfringens nitrite + H2O + oxidized ferredoxin
-
 ?
1.7.7.2 nitrate + reduced ferredoxin nitrate fermentation, ferredoxin is utilized as direct electron donor in nitrate reduction Clostridium perfringens nitrite + oxidized ferredoxin
-
 ?
1.7.7.2 nitrate + reduced methyl viologen nitrite formation 10times higher at pH 9 than at pH 8 Clostridium perfringens nitrite + oxidized methyl viologen
-
 ?
1.7.7.2 nitrate + reduced methyl viologen enzyme uses also reduced methyl viologen as reductant Clostridium perfringens nitrite + oxidized methyl viologen
-
 ?
1.7.7.2 nitrate + reduced methyl viologen enzyme also has methyl viologen-linked activity Clostridium perfringens nitrite + oxidized methyl viologen
-
 ?

Subunits

EC Number Subunits Comment Organism
1.7.7.2 monomer 1 * 90000, SDS-PAGE Clostridium perfringens

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.7.7.2 37
-
 assay at Clostridium perfringens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.7.7.2 9
-
 reduced methyl viologen as electron donor Clostridium perfringens

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.7.7.2 8.3 9.8 at pH 8.3 and 9.8: about 50% of activity maximum Clostridium perfringens