BRENDA - Enzyme Database

Partial purification and characterization of polyphenol oxidase from banana (Musa sapientum L.) peel

Yang, C.P.; Fujita, S.; Kohno, K.; Kusubayashi, A.; Ashrafuzzaman, M.; Hayashi, N.; J. Agric. Food Chem. 49, 1446-1449 (2001)

Data extracted from this reference:

Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
1.10.3.1
ascorbic acid
peel enzyme, 1 mM, complete inhibition
Musa x paradisiaca
1.10.3.1
CN-
peel enzyme, 1 mM, 89% inhibition
Musa x paradisiaca
1.10.3.1
cysteine
peel enzyme, 1 mM, complete inhibition
Musa x paradisiaca
1.10.3.1
diethyldithiocarbamate
peel enzyme, 1 mM, complete inhibition
Musa x paradisiaca
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.10.3.1
41000
-
peel enzyme, gel filtration
Musa x paradisiaca
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
1.10.3.1
Musa x paradisiaca
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
1.10.3.1
-
Musa x paradisiaca
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
1.10.3.1
peel
-
Musa x paradisiaca
-
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
1.10.3.1
additional information
-
10512.0 units/mg, 1 unit is defined as absorbance change at 420 nm of 0.1 per min and per ml enzyme solution
Musa x paradisiaca
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.10.3.1
catechol + 1/2 O2
peel enzyme, 34% of activity with dopamine
440437
Musa x paradisiaca
1,2-benzoquinone + H2O
-
440437
Musa x paradisiaca
-
1.10.3.1
chlorogenic acid + O2
peel enzyme, 5.3% of activity with dopamine
440437
Musa x paradisiaca
?
-
-
-
-
1.10.3.1
D-catechin + O2
peel enzyme, 11.5% of activity with dopamine
440437
Musa x paradisiaca
?
-
-
-
-
1.10.3.1
dopamine + O2
-
440437
Musa x paradisiaca
4-(2-aminoethyl)-1,2-benzoquinone + 2 H2O
-
440437
Musa x paradisiaca
-
1.10.3.1
epicatechin + O2
peel enzyme, 9.3% of activity with dopamine
440437
Musa x paradisiaca
?
-
-
-
-
1.10.3.1
L-dopa + 1/2 O2
peel enzyme, 8.0% of activity with dopamine
440437
Musa x paradisiaca
L-dopaquinone + H2O
-
440437
Musa x paradisiaca
-
1.10.3.1
pyrogallol + O2
peel enzyme, 1.4% of activity with dopamine
440437
Musa x paradisiaca
?
-
-
-
-
Temperature Optimum [°C]
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
1.10.3.1
30
-
-
Musa x paradisiaca
Temperature Stability [°C]
EC Number
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
1.10.3.1
60
-
peel enzyme, loss of 10% activity after 30 min
Musa x paradisiaca
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.10.3.1
6.5
-
-
Musa x paradisiaca
pH Stability
EC Number
pH Stability
pH Stability Maximum
Commentary
Organism
1.10.3.1
5
11
peel enzyme
Musa x paradisiaca
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
1.10.3.1
ascorbic acid
peel enzyme, 1 mM, complete inhibition
Musa x paradisiaca
1.10.3.1
CN-
peel enzyme, 1 mM, 89% inhibition
Musa x paradisiaca
1.10.3.1
cysteine
peel enzyme, 1 mM, complete inhibition
Musa x paradisiaca
1.10.3.1
diethyldithiocarbamate
peel enzyme, 1 mM, complete inhibition
Musa x paradisiaca
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.10.3.1
41000
-
peel enzyme, gel filtration
Musa x paradisiaca
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
1.10.3.1
-
Musa x paradisiaca
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
1.10.3.1
peel
-
Musa x paradisiaca
-
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
1.10.3.1
additional information
-
10512.0 units/mg, 1 unit is defined as absorbance change at 420 nm of 0.1 per min and per ml enzyme solution
Musa x paradisiaca
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.10.3.1
catechol + 1/2 O2
peel enzyme, 34% of activity with dopamine
440437
Musa x paradisiaca
1,2-benzoquinone + H2O
-
440437
Musa x paradisiaca
-
1.10.3.1
chlorogenic acid + O2
peel enzyme, 5.3% of activity with dopamine
440437
Musa x paradisiaca
?
-
-
-
-
1.10.3.1
D-catechin + O2
peel enzyme, 11.5% of activity with dopamine
440437
Musa x paradisiaca
?
-
-
-
-
1.10.3.1
dopamine + O2
-
440437
Musa x paradisiaca
4-(2-aminoethyl)-1,2-benzoquinone + 2 H2O
-
440437
Musa x paradisiaca
-
1.10.3.1
epicatechin + O2
peel enzyme, 9.3% of activity with dopamine
440437
Musa x paradisiaca
?
-
-
-
-
1.10.3.1
L-dopa + 1/2 O2
peel enzyme, 8.0% of activity with dopamine
440437
Musa x paradisiaca
L-dopaquinone + H2O
-
440437
Musa x paradisiaca
-
1.10.3.1
pyrogallol + O2
peel enzyme, 1.4% of activity with dopamine
440437
Musa x paradisiaca
?
-
-
-
-
Temperature Optimum [°C] (protein specific)
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
1.10.3.1
30
-
-
Musa x paradisiaca
Temperature Stability [°C] (protein specific)
EC Number
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
1.10.3.1
60
-
peel enzyme, loss of 10% activity after 30 min
Musa x paradisiaca
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.10.3.1
6.5
-
-
Musa x paradisiaca
pH Stability (protein specific)
EC Number
pH Stability
pH Stability Maximum
Commentary
Organism
1.10.3.1
5
11
peel enzyme
Musa x paradisiaca