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Literature summary extracted from

  • Paul, B.; Gowda, L.R.
    Purification and characterization of a polyphenol oxidase from the seeds of field bean (Dolichos lablab) (2000), J. Agric. Food Chem., 48, 3839-3846.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.10.3.1 2-hydroxy-2,4,6-cycloheptatrien-1-one trivial name tropolone Lablab purpureus
1.10.3.1 ascorbic acid
-
Lablab purpureus
1.10.3.1 cysteine
-
Lablab purpureus
1.10.3.1 Metabisulfite
-
Lablab purpureus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.10.3.1 1.18
-
L-Dopa
-
Lablab purpureus
1.10.3.1 4
-
4-methylcatechol
-
Lablab purpureus
1.10.3.1 10.5
-
catechol
-
Lablab purpureus
1.10.3.1 12.5
-
pyrogallol
-
Lablab purpureus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.10.3.1 30000
-
4 * 30000, SDS-PAGE Lablab purpureus
1.10.3.1 120000
-
gel filtration Lablab purpureus

Organism

EC Number Organism UniProt Comment Textmining
1.10.3.1 Lablab purpureus
-
field bean
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.10.3.1 ammonium sulfate, DEAE-Sephacel, Phenyl-agarose, Sephadex G-100 Lablab purpureus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.10.3.1 additional information
-
68077.0 units/mg, 1 unit is defined as absorbance change of 0.001 per min at 25°C Lablab purpureus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.10.3.1 4-methylcatechol + O2 140% of activity with catechol Lablab purpureus 4-methyl-1,2-benzoquinone
-
?
1.10.3.1 catechol + 1/2 O2
-
Lablab purpureus 1,2-benzoquinone + H2O
-
?
1.10.3.1 L-dopa + 1/2 O2 22.65 of activity with catechol Lablab purpureus L-dopaquinone + H2O
-
?
1.10.3.1 pyrogallol + O2 24% of activity with catechol Lablab purpureus ?
-
?

Subunits

EC Number Subunits Comment Organism
1.10.3.1 tetramer 4 * 30000, SDS-PAGE Lablab purpureus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.10.3.1 4
-
catechol oxidation Lablab purpureus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.10.3.1 3 5.5 50% activity at pH 3.5 and pH 5.5, 25% activity at pH 3.0 Lablab purpureus

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1.10.3.1 0.00058
-
tropolone
-
Lablab purpureus
1.10.3.1 0.0055
-
Metabisulfite
-
Lablab purpureus
1.10.3.1 0.0075
-
ascorbic acid
-
Lablab purpureus
1.10.3.1 19.5
-
cysteine
-
Lablab purpureus