BRENDA - Enzyme Database

Purification and characterization of polyphenol oxidase from banana (Musa sapientum L.) pulp

Yang, C.P.; Fujita, S.; Ashrafuzzaman, M.; Nakamura, N.; Hayashi, N.; J. Agric. Food Chem. 48, 2732-2735 (2000)

Data extracted from this reference:

Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
1.10.3.1
ascorbic acid
pulp enzyme, 1 mM, complete inhibition
Musa x paradisiaca
1.10.3.1
CN-
pulp enzyme, 1 mM, 80% inhibition
Musa x paradisiaca
1.10.3.1
cysteine
pulp enzyme, 1 mM, complete inhibition
Musa x paradisiaca
1.10.3.1
diethyldithiocarbamate
pulp enzyme, 1 mM, complete inhibition
Musa x paradisiaca
1.10.3.1
additional information
not inhibited by 10 mM EDTA, Cu2+, Mn2+, Zn2+, and Ba2+
Musa x paradisiaca
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.10.3.1
2.8
-
dopamine
-
Musa x paradisiaca
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.10.3.1
41000
-
pulp enzyme, gel filtration
Musa x paradisiaca
1.10.3.1
42000
-
1 * 42000, SDS-PAGE
Musa x paradisiaca
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
1.10.3.1
Musa x paradisiaca
-
banana
-
Purification (Commentary)
EC Number
Commentary
Organism
1.10.3.1
ammonium sulfate, DEAE-toyopearl, butyl-toyopearl, Super Q toyopearl, hydroxyapatite, toyopearl HW 55
Musa x paradisiaca
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
1.10.3.1
pulp
-
Musa x paradisiaca
-
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
1.10.3.1
additional information
-
13160.0 units/mg, 1 unit is defined as absorbance change at 420 nm of 0.1 per min
Musa x paradisiaca
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.10.3.1
caffeic acid + 1/2 O2
2% of activity with dopamine
440433
Musa x paradisiaca
caffeoyl quinone + H2O
-
-
-
?
1.10.3.1
catechol + 1/2 O2
pulp enzyme, 54% of activity with dopamine
440433
Musa x paradisiaca
1,2-benzoquinone + H2O
-
440433
Musa x paradisiaca
?
1.10.3.1
chlorogenic acid + O2
pulp enzyme, 24.5% of activity with dopamine
440433
Musa x paradisiaca
?
-
-
-
?
1.10.3.1
D-catechin + O2
pulp enzyme, 35.6% of activity with dopamine
440433
Musa x paradisiaca
?
-
-
-
?
1.10.3.1
dopamine + O2
-
440433
Musa x paradisiaca
4-(2-aminoethyl)-1,2-benzoquinone + 2 H2O
-
440433
Musa x paradisiaca
?
1.10.3.1
epicatechin + O2
pulp enzyme, 22.7% of activity with dopamine
440433
Musa x paradisiaca
?
-
-
-
?
1.10.3.1
L-dopa + 1/2 O2
pulp enzyme, 12.3% of activity with dopamine
440433
Musa x paradisiaca
L-dopaquinone + H2O
-
440433
Musa x paradisiaca
?
1.10.3.1
pyrogallol + O2
pulp enzyme, 5.5% of activity with dopamine
440433
Musa x paradisiaca
?
-
-
-
?
Subunits
EC Number
Subunits
Commentary
Organism
1.10.3.1
monomer
1 * 42000, SDS-PAGE
Musa x paradisiaca
Temperature Optimum [°C]
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
1.10.3.1
30
-
-
Musa x paradisiaca
Temperature Stability [°C]
EC Number
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
1.10.3.1
70
-
pulp enzyme, loss of 20% activity after 10 min
Musa x paradisiaca
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.10.3.1
6.5
-
-
Musa x paradisiaca
pH Stability
EC Number
pH Stability
pH Stability Maximum
Commentary
Organism
1.10.3.1
5
11
10% loss of activity after 48 h between pH 5.0 and 11.0
Musa x paradisiaca
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
1.10.3.1
ascorbic acid
pulp enzyme, 1 mM, complete inhibition
Musa x paradisiaca
1.10.3.1
CN-
pulp enzyme, 1 mM, 80% inhibition
Musa x paradisiaca
1.10.3.1
cysteine
pulp enzyme, 1 mM, complete inhibition
Musa x paradisiaca
1.10.3.1
diethyldithiocarbamate
pulp enzyme, 1 mM, complete inhibition
Musa x paradisiaca
1.10.3.1
additional information
not inhibited by 10 mM EDTA, Cu2+, Mn2+, Zn2+, and Ba2+
Musa x paradisiaca
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.10.3.1
2.8
-
dopamine
-
Musa x paradisiaca
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.10.3.1
41000
-
pulp enzyme, gel filtration
Musa x paradisiaca
1.10.3.1
42000
-
1 * 42000, SDS-PAGE
Musa x paradisiaca
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
1.10.3.1
ammonium sulfate, DEAE-toyopearl, butyl-toyopearl, Super Q toyopearl, hydroxyapatite, toyopearl HW 55
Musa x paradisiaca
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
1.10.3.1
pulp
-
Musa x paradisiaca
-
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
1.10.3.1
additional information
-
13160.0 units/mg, 1 unit is defined as absorbance change at 420 nm of 0.1 per min
Musa x paradisiaca
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.10.3.1
caffeic acid + 1/2 O2
2% of activity with dopamine
440433
Musa x paradisiaca
caffeoyl quinone + H2O
-
-
-
?
1.10.3.1
catechol + 1/2 O2
pulp enzyme, 54% of activity with dopamine
440433
Musa x paradisiaca
1,2-benzoquinone + H2O
-
440433
Musa x paradisiaca
?
1.10.3.1
chlorogenic acid + O2
pulp enzyme, 24.5% of activity with dopamine
440433
Musa x paradisiaca
?
-
-
-
?
1.10.3.1
D-catechin + O2
pulp enzyme, 35.6% of activity with dopamine
440433
Musa x paradisiaca
?
-
-
-
?
1.10.3.1
dopamine + O2
-
440433
Musa x paradisiaca
4-(2-aminoethyl)-1,2-benzoquinone + 2 H2O
-
440433
Musa x paradisiaca
?
1.10.3.1
epicatechin + O2
pulp enzyme, 22.7% of activity with dopamine
440433
Musa x paradisiaca
?
-
-
-
?
1.10.3.1
L-dopa + 1/2 O2
pulp enzyme, 12.3% of activity with dopamine
440433
Musa x paradisiaca
L-dopaquinone + H2O
-
440433
Musa x paradisiaca
?
1.10.3.1
pyrogallol + O2
pulp enzyme, 5.5% of activity with dopamine
440433
Musa x paradisiaca
?
-
-
-
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
1.10.3.1
monomer
1 * 42000, SDS-PAGE
Musa x paradisiaca
Temperature Optimum [°C] (protein specific)
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
1.10.3.1
30
-
-
Musa x paradisiaca
Temperature Stability [°C] (protein specific)
EC Number
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
1.10.3.1
70
-
pulp enzyme, loss of 20% activity after 10 min
Musa x paradisiaca
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.10.3.1
6.5
-
-
Musa x paradisiaca
pH Stability (protein specific)
EC Number
pH Stability
pH Stability Maximum
Commentary
Organism
1.10.3.1
5
11
10% loss of activity after 48 h between pH 5.0 and 11.0
Musa x paradisiaca