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Literature summary extracted from

  • Dry, I.B.; Robinson, S.P.
    Molecular cloning and characterisation of grape berry polyphenol oxidase (1994), Plant Mol. Biol., 26, 495-502.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.10.3.1 cloning of cDNA Vitis vinifera

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.10.3.1 40000
-
 1 * 40000, mature enzyme, SDS-PAGE Vitis vinifera
1.10.3.1 56700
-
 1 * 56700, mature enzyme, enzyme is synthesized as a 67300 Da precursor protein, predicted from nucleotide sequence Vitis vinifera

Organism

EC Number Organism UniProt Comment Textmining
1.10.3.1 Vitis vinifera P43311 sultana grape
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.10.3.1 hexadecyltrimethylammonium bromide extraction, ammonium sulfate, Q-Sepharose, Phenyl-Sepharose, hydroxylapatite Vitis vinifera

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.10.3.1 berry high expression levels in developing berries and leaves Vitis vinifera
-
1.10.3.1 leaf
-
 Vitis vinifera
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.10.3.1 66
-
-
 Vitis vinifera

Subunits

EC Number Subunits Comment Organism
1.10.3.1 monomer 1 * 40000, mature enzyme, SDS-PAGE Vitis vinifera
1.10.3.1 monomer 1 * 56700, mature enzyme, enzyme is synthesized as a 67300 Da precursor protein, predicted from nucleotide sequence Vitis vinifera