BRENDA - Enzyme Database

Properties of polyphenol oxidase from Alternaria tenuis

Motoda, S.; J. Ferment. Technol. 57, 79-85 (1979)
No PubMed abstract available

Data extracted from this reference:

Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
1.10.3.1
1-Phenyl-2-thiourea
-
Alternaria alternata
1.10.3.1
2-mercaptobenzothiazole
1 mM, 94% inhibition
Alternaria alternata
1.10.3.1
2-mercaptoethanol
1 mM, 97% inhibition
Alternaria alternata
1.10.3.1
8-hydroxyquinoline
-
Alternaria alternata
1.10.3.1
Ag+
1 mM, 60% inhibition
Alternaria alternata
1.10.3.1
Al3+
1 mM, 89% inhibition
Alternaria alternata
1.10.3.1
ascorbic acid
-
Alternaria alternata
1.10.3.1
CN-
1 mM, 82% inhibition
Alternaria alternata
1.10.3.1
Cuprizone
-
Alternaria alternata
1.10.3.1
cysteine
1 mM, 92% inhibition
Alternaria alternata
1.10.3.1
diethyldithiocarbamate
-
Alternaria alternata
1.10.3.1
Hg2+
1 mM, 84% inhibition
Alternaria alternata
1.10.3.1
isoascorbic acid
1 mM, 99% inhibition
Alternaria alternata
1.10.3.1
Metabisulfite
1 mM, 95% inhibition
Alternaria alternata
1.10.3.1
NaHSO3
-
Alternaria alternata
1.10.3.1
o-phenanthroline
-
Alternaria alternata
1.10.3.1
Sn2+
1 mM, 99% inhibition
Alternaria alternata
1.10.3.1
Sodium azide
-
Alternaria alternata
1.10.3.1
Sodium bisulfite
1 mM, 97% inhibition
Alternaria alternata
1.10.3.1
Thiourea
1 mM, 59% inhibition
Alternaria alternata
1.10.3.1
Xanthogenate
1 mM, 94% inhibition
Alternaria alternata
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.10.3.1
0.47
-
D-catechin
-
Alternaria alternata
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
1.10.3.1
Co2+
1 mM, slight stimulation
Alternaria alternata
1.10.3.1
Cu+
1 mM, 234% activity
Alternaria alternata
1.10.3.1
Cu2+
1 mM, 241% activity, enzyme contains 0.24% copper
Alternaria alternata
1.10.3.1
Pb2+
1 mM, slight stimulation
Alternaria alternata
1.10.3.1
Zn2+
1 mM, slight stimulation
Alternaria alternata
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
1.10.3.1
Alternaria alternata
-
-
-
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
1.10.3.1
culture filtrate
-
Alternaria alternata
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.10.3.1
2,3-dihydroxybenzoic acid + O2
-
440427
Alternaria alternata
?
-
-
-
?
1.10.3.1
3,4,5-trihydroxybenzoic acid + O2
trivial name gallic acid
440427
Alternaria alternata
?
-
-
-
?
1.10.3.1
4-methylcatechol + O2
-
440427
Alternaria alternata
4-methyl-o-benzoquinone
-
440427
Alternaria alternata
?
1.10.3.1
caffeic acid + 1/2 O2
-
440427
Alternaria alternata
caffeoyl quinone + H2O
-
440427
Alternaria alternata
?
1.10.3.1
catechol + 1/2 O2
no activity with phenol, 4-cresol, L-tyrosine and 4-coumaric acid
440427
Alternaria alternata
1,2-benzoquinone + H2O
-
440427
Alternaria alternata
?
1.10.3.1
chlorogenic acid + O2
-
440427
Alternaria alternata
?
-
-
-
?
1.10.3.1
D-catechin + O2
-
440427
Alternaria alternata
?
-
-
-
?
1.10.3.1
dopamine + O2
-
440427
Alternaria alternata
4-(2-aminoethyl)-1,2-benzoquinone + 2 H2O
-
440427
Alternaria alternata
?
1.10.3.1
epinephrine + O2
-
440427
Alternaria alternata
?
-
-
-
?
1.10.3.1
ferulic acid + O2
-
440427
Alternaria alternata
?
-
-
-
?
1.10.3.1
L-catechin + O2
-
440427
Alternaria alternata
?
-
-
-
?
1.10.3.1
phloroglucinol + O2
-
440427
Alternaria alternata
?
-
-
-
?
1.10.3.1
pyrogallol + O2
-
440427
Alternaria alternata
?
-
-
-
?
1.10.3.1
quercetin + O2
-
440427
Alternaria alternata
?
-
-
-
?
1.10.3.1
rutin + O2
-
440427
Alternaria alternata
?
-
-
-
?
1.10.3.1
shikimic acid + O2
-
440427
Alternaria alternata
?
-
-
-
?
1.10.3.1
tannic acid + O2
-
440427
Alternaria alternata
?
-
-
-
?
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
1.10.3.1
1-Phenyl-2-thiourea
-
Alternaria alternata
1.10.3.1
2-mercaptobenzothiazole
1 mM, 94% inhibition
Alternaria alternata
1.10.3.1
2-mercaptoethanol
1 mM, 97% inhibition
Alternaria alternata
1.10.3.1
8-hydroxyquinoline
-
Alternaria alternata
1.10.3.1
Ag+
1 mM, 60% inhibition
Alternaria alternata
1.10.3.1
Al3+
1 mM, 89% inhibition
Alternaria alternata
1.10.3.1
ascorbic acid
-
Alternaria alternata
1.10.3.1
CN-
1 mM, 82% inhibition
Alternaria alternata
1.10.3.1
Cuprizone
-
Alternaria alternata
1.10.3.1
cysteine
1 mM, 92% inhibition
Alternaria alternata
1.10.3.1
diethyldithiocarbamate
-
Alternaria alternata
1.10.3.1
Hg2+
1 mM, 84% inhibition
Alternaria alternata
1.10.3.1
isoascorbic acid
1 mM, 99% inhibition
Alternaria alternata
1.10.3.1
Metabisulfite
1 mM, 95% inhibition
Alternaria alternata
1.10.3.1
NaHSO3
-
Alternaria alternata
1.10.3.1
o-phenanthroline
-
Alternaria alternata
1.10.3.1
Sn2+
1 mM, 99% inhibition
Alternaria alternata
1.10.3.1
Sodium azide
-
Alternaria alternata
1.10.3.1
Sodium bisulfite
1 mM, 97% inhibition
Alternaria alternata
1.10.3.1
Thiourea
1 mM, 59% inhibition
Alternaria alternata
1.10.3.1
Xanthogenate
1 mM, 94% inhibition
Alternaria alternata
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.10.3.1
0.47
-
D-catechin
-
Alternaria alternata
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
1.10.3.1
Co2+
1 mM, slight stimulation
Alternaria alternata
1.10.3.1
Cu+
1 mM, 234% activity
Alternaria alternata
1.10.3.1
Cu2+
1 mM, 241% activity, enzyme contains 0.24% copper
Alternaria alternata
1.10.3.1
Pb2+
1 mM, slight stimulation
Alternaria alternata
1.10.3.1
Zn2+
1 mM, slight stimulation
Alternaria alternata
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
1.10.3.1
culture filtrate
-
Alternaria alternata
-
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.10.3.1
2,3-dihydroxybenzoic acid + O2
-
440427
Alternaria alternata
?
-
-
-
?
1.10.3.1
3,4,5-trihydroxybenzoic acid + O2
trivial name gallic acid
440427
Alternaria alternata
?
-
-
-
?
1.10.3.1
4-methylcatechol + O2
-
440427
Alternaria alternata
4-methyl-o-benzoquinone
-
440427
Alternaria alternata
?
1.10.3.1
caffeic acid + 1/2 O2
-
440427
Alternaria alternata
caffeoyl quinone + H2O
-
440427
Alternaria alternata
?
1.10.3.1
catechol + 1/2 O2
no activity with phenol, 4-cresol, L-tyrosine and 4-coumaric acid
440427
Alternaria alternata
1,2-benzoquinone + H2O
-
440427
Alternaria alternata
?
1.10.3.1
chlorogenic acid + O2
-
440427
Alternaria alternata
?
-
-
-
?
1.10.3.1
D-catechin + O2
-
440427
Alternaria alternata
?
-
-
-
?
1.10.3.1
dopamine + O2
-
440427
Alternaria alternata
4-(2-aminoethyl)-1,2-benzoquinone + 2 H2O
-
440427
Alternaria alternata
?
1.10.3.1
epinephrine + O2
-
440427
Alternaria alternata
?
-
-
-
?
1.10.3.1
ferulic acid + O2
-
440427
Alternaria alternata
?
-
-
-
?
1.10.3.1
L-catechin + O2
-
440427
Alternaria alternata
?
-
-
-
?
1.10.3.1
phloroglucinol + O2
-
440427
Alternaria alternata
?
-
-
-
?
1.10.3.1
pyrogallol + O2
-
440427
Alternaria alternata
?
-
-
-
?
1.10.3.1
quercetin + O2
-
440427
Alternaria alternata
?
-
-
-
?
1.10.3.1
rutin + O2
-
440427
Alternaria alternata
?
-
-
-
?
1.10.3.1
shikimic acid + O2
-
440427
Alternaria alternata
?
-
-
-
?
1.10.3.1
tannic acid + O2
-
440427
Alternaria alternata
?
-
-
-
?