BRENDA - Enzyme Database

Isolation and characterization of polyphenol oxidase isozymes of clingstone peach

Wong, T.C.; Luh, B.S.; Whitaker, J.R.; Plant Physiol. 48, 19-23 (1971)

Data extracted from this reference:

Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
1.10.3.1
ascorbic acid
0.066 mM, 26%, 59% and 96% inhibition of isoenzymes B, C, and D, respectively
Prunus persica
1.10.3.1
CN-
0.165 mM, 84%, 70%, 100% and 40% inhibition of isoenzymes A, B, C and D, respectively
Prunus persica
1.10.3.1
diethyldithiocarbamate
0.066 mM, 31%, 40%, 36% and 84% inhibition of isoenzymes A, B, C, and D, respectively
Prunus persica
1.10.3.1
EDTA
100 mM, 33%, 60%, 81% and 35% inhibition of isoenzymes A, B, C and D, respectively
Prunus persica
1.10.3.1
glutathione
0.066 mM, 2%, 22% and 84% inhibition of isoenzymes B, C, and D, respectively
Prunus persica
1.10.3.1
NaCl
800 mM, 48%, 47%, 55% and 93% inhibition of isoenzymes A, B, C, and D, respectively
Prunus persica
1.10.3.1
NaHSO3
0.066 mM, 64%, 50% and 27% inhibition of isoenzymes B, C, and D, respectively
Prunus persica
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.10.3.1
4.2
-
catechol
isoenzyme B
Prunus persica
1.10.3.1
6.6
-
catechol
isoenzyme A
Prunus persica
1.10.3.1
7
-
catechol
isoenzyme C
Prunus persica
1.10.3.1
36
-
catechol
isoenzyme D
Prunus persica
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
1.10.3.1
Prunus persica
-
peach
-
Purification (Commentary)
EC Number
Commentary
Organism
1.10.3.1
isoenzymes A, B, C and D, acetone precipitate, DEAE-cellulose
Prunus persica
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
1.10.3.1
fruit
-
Prunus persica
-
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [mol/min/mg]
Specific Activity Maximum [mol/min/mg]
Commentary
Organism
1.10.3.1
additional information
-
isoenzyme A, 0.0016 units, isoenzyme B, 0.011 units, isoenzyme C, 0.0013 units/mg, isoenzyme D, 0.0028 units, 1 unit is defined as absorbance change at 420 nm of 0.001 per min
Prunus persica
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.10.3.1
3,4,5-trihydroxybenzoic acid + O2
isoenzymes A-C
440426
Prunus persica
?
-
-
-
?
1.10.3.1
3,4,5-trihydroxybenzoic acid + O2
trivial name gallic acid
440426
Prunus persica
?
-
-
-
?
1.10.3.1
4-methylcatechol + O2
isoenzymes A-D
440426
Prunus persica
4-methyl-o-benzoquinone
-
440426
Prunus persica
?
1.10.3.1
caffeic acid + 1/2 O2
isoenzymes C and D
440426
Prunus persica
caffeoyl quinone + H2O
-
440426
Prunus persica
?
1.10.3.1
catechol + 1/2 O2
isoenzymes A, B, C and D show no phenolase activity
440426
Prunus persica
1,2-benzoquinone + H2O
-
440426
Prunus persica
?
1.10.3.1
chlorogenic acid + O2
isoenzymes A-D
440426
Prunus persica
?
-
-
-
?
1.10.3.1
D-catechin + O2
isoenzymes A-D
440426
Prunus persica
?
-
-
-
?
1.10.3.1
dopamine + O2
isoenzymes A-D
440426
Prunus persica
4-(2-aminoethyl)-1,2-benzoquinone + 2 H2O
-
440426
Prunus persica
?
1.10.3.1
L-dopa + 1/2 O2
isoenzymes A-D
440426
Prunus persica
L-dopaquinone + H2O
-
440426
Prunus persica
?
1.10.3.1
protocatechuic acid + O2
isoenzyme C
440426
Prunus persica
?
-
-
-
?
Temperature Stability [C]
EC Number
Temperature Stability Minimum [C]
Temperature Stability Maximum [C]
Commentary
Organism
1.10.3.1
55
-
half-lives of isoenzymes A, B and D: 5.4, 14,6 and 14.1 min respectively, isoenzyme C: stable for 50 min at 55C, half-life at 76C: 2.2 min
Prunus persica
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.10.3.1
6.5
-
isoenzyme B
Prunus persica
1.10.3.1
6.8
-
isoenzyme A
Prunus persica
1.10.3.1
7
-
isoenzyme D
Prunus persica
1.10.3.1
7.2
-
isoenzyme C
Prunus persica
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
1.10.3.1
ascorbic acid
0.066 mM, 26%, 59% and 96% inhibition of isoenzymes B, C, and D, respectively
Prunus persica
1.10.3.1
CN-
0.165 mM, 84%, 70%, 100% and 40% inhibition of isoenzymes A, B, C and D, respectively
Prunus persica
1.10.3.1
diethyldithiocarbamate
0.066 mM, 31%, 40%, 36% and 84% inhibition of isoenzymes A, B, C, and D, respectively
Prunus persica
1.10.3.1
EDTA
100 mM, 33%, 60%, 81% and 35% inhibition of isoenzymes A, B, C and D, respectively
Prunus persica
1.10.3.1
glutathione
0.066 mM, 2%, 22% and 84% inhibition of isoenzymes B, C, and D, respectively
Prunus persica
1.10.3.1
NaCl
800 mM, 48%, 47%, 55% and 93% inhibition of isoenzymes A, B, C, and D, respectively
Prunus persica
1.10.3.1
NaHSO3
0.066 mM, 64%, 50% and 27% inhibition of isoenzymes B, C, and D, respectively
Prunus persica
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.10.3.1
4.2
-
catechol
isoenzyme B
Prunus persica
1.10.3.1
6.6
-
catechol
isoenzyme A
Prunus persica
1.10.3.1
7
-
catechol
isoenzyme C
Prunus persica
1.10.3.1
36
-
catechol
isoenzyme D
Prunus persica
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
1.10.3.1
isoenzymes A, B, C and D, acetone precipitate, DEAE-cellulose
Prunus persica
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
1.10.3.1
fruit
-
Prunus persica
-
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [mol/min/mg]
Specific Activity Maximum [mol/min/mg]
Commentary
Organism
1.10.3.1
additional information
-
isoenzyme A, 0.0016 units, isoenzyme B, 0.011 units, isoenzyme C, 0.0013 units/mg, isoenzyme D, 0.0028 units, 1 unit is defined as absorbance change at 420 nm of 0.001 per min
Prunus persica
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.10.3.1
3,4,5-trihydroxybenzoic acid + O2
isoenzymes A-C
440426
Prunus persica
?
-
-
-
?
1.10.3.1
3,4,5-trihydroxybenzoic acid + O2
trivial name gallic acid
440426
Prunus persica
?
-
-
-
?
1.10.3.1
4-methylcatechol + O2
isoenzymes A-D
440426
Prunus persica
4-methyl-o-benzoquinone
-
440426
Prunus persica
?
1.10.3.1
caffeic acid + 1/2 O2
isoenzymes C and D
440426
Prunus persica
caffeoyl quinone + H2O
-
440426
Prunus persica
?
1.10.3.1
catechol + 1/2 O2
isoenzymes A, B, C and D show no phenolase activity
440426
Prunus persica
1,2-benzoquinone + H2O
-
440426
Prunus persica
?
1.10.3.1
chlorogenic acid + O2
isoenzymes A-D
440426
Prunus persica
?
-
-
-
?
1.10.3.1
D-catechin + O2
isoenzymes A-D
440426
Prunus persica
?
-
-
-
?
1.10.3.1
dopamine + O2
isoenzymes A-D
440426
Prunus persica
4-(2-aminoethyl)-1,2-benzoquinone + 2 H2O
-
440426
Prunus persica
?
1.10.3.1
L-dopa + 1/2 O2
isoenzymes A-D
440426
Prunus persica
L-dopaquinone + H2O
-
440426
Prunus persica
?
1.10.3.1
protocatechuic acid + O2
isoenzyme C
440426
Prunus persica
?
-
-
-
?
Temperature Stability [C] (protein specific)
EC Number
Temperature Stability Minimum [C]
Temperature Stability Maximum [C]
Commentary
Organism
1.10.3.1
55
-
half-lives of isoenzymes A, B and D: 5.4, 14,6 and 14.1 min respectively, isoenzyme C: stable for 50 min at 55C, half-life at 76C: 2.2 min
Prunus persica
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.10.3.1
6.5
-
isoenzyme B
Prunus persica
1.10.3.1
6.8
-
isoenzyme A
Prunus persica
1.10.3.1
7
-
isoenzyme D
Prunus persica
1.10.3.1
7.2
-
isoenzyme C
Prunus persica