EC Number | Cloned (Comment) | Organism |
---|---|---|
1.13.12.4 | expressed in Escherichia coli | Mycolicibacterium smegmatis |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
1.13.12.4 | G99A | (S)-lactate binds tighter to the G99A-mutant than to wild-type | Mycolicibacterium smegmatis |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
1.13.12.4 | (R)-lactate | competitive inhibitor | Mycolicibacterium smegmatis | |
1.13.12.4 | 2-Hydroxy-3-butynoate | suicide substrate | Mycolicibacterium smegmatis |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.13.12.4 | 0.014 | - |
(S)-lactate | calculated constant, G99A mutant | Mycolicibacterium smegmatis | |
1.13.12.4 | 0.02 | - |
(S)-lactate | observed constant, G99A mutant | Mycolicibacterium smegmatis | |
1.13.12.4 | 0.055 | - |
O2 | calculated constant, wild-type | Mycolicibacterium smegmatis | |
1.13.12.4 | 0.071 | - |
O2 | observed constant, wild-type | Mycolicibacterium smegmatis | |
1.13.12.4 | 0.83 | - |
O2 | observed constant, G99A mutant | Mycolicibacterium smegmatis | |
1.13.12.4 | 0.92 | - |
O2 | calculated constant, G99A mutant | Mycolicibacterium smegmatis | |
1.13.12.4 | 22 | - |
(S)-lactate | wild-type | Mycolicibacterium smegmatis |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
1.13.12.4 | 43000 | - |
8 * 43000, amino acid analysis | Mycolicibacterium smegmatis |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.13.12.4 | (S)-lactate + O2 | Mycolicibacterium smegmatis | - |
acetate + CO2 + H2O | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.13.12.4 | Mycolicibacterium smegmatis | - |
- |
- |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.13.12.4 | (S)-lactate + O2 | - |
Mycolicibacterium smegmatis | acetate + CO2 + H2O | - |
? | |
1.13.12.4 | (S)-lactate + O2 | G99A-mutant, no decarboxylation | Mycolicibacterium smegmatis | pyruvate + H2O2 | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
1.13.12.4 | octamer | 8 * 43000, amino acid analysis | Mycolicibacterium smegmatis |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.13.12.4 | 0.0148 | - |
(S)-lactate | - |
Mycolicibacterium smegmatis |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.13.12.4 | FMN | one FMN per subunit | Mycolicibacterium smegmatis |