Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Streitenberger, S.A.; Lopez-Mas, J.A.; Sanchez-Ferrer, A.; Garcia-Carmona, F.
    Non-linear slow-binding inhibition of Aerococcus viridans lactate oxidase by Cibacron Blue 3GA (2001), J. Enzyme Inhib., 16, 301-312.
    View publication on PubMed

Application

EC Number Application Comment Organism
1.1.3.15 medicine enzyme is used in clinical chemistry for the determination of (S)-lactate in blood in some pathological diseases such as diabetes, heart diseases and shock syndrome Aerococcus viridans

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.3.15 (R)-lactate at pH 7.0 Aerococcus viridans
1.1.3.15 2-Hydroxybutyrate at pH 7.0 Aerococcus viridans
1.1.3.15 Cibacron blue 3GA at a concentration higher than 0.001 mM is a normal competitive inhibitor, at concentrations below 0.001 mM the inhibition is time-, dye- and pH-dependent Aerococcus viridans
1.1.3.15 glycolate
-
 Aerococcus viridans
1.1.3.15 L-Mandelate at pH 7.0 Aerococcus viridans
1.1.3.15 oxalate at pH 7.0 Aerococcus viridans

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.3.15 0.175
-
 (S)-lactate
-
 Aerococcus viridans

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.3.15 48200
-
 4 * 48200, SDS-PAGE Aerococcus viridans
1.1.3.15 187300
-
 gel filtration Aerococcus viridans

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.3.15 (S)-lactate + O2 Aerococcus viridans no decarboxylation pyruvate + H2O2
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.1.3.15 Aerococcus viridans
-
 strain ATCC 11563
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.3.15
-
 Aerococcus viridans

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.3.15 (S)-lactate + O2 no decarboxylation Aerococcus viridans pyruvate + H2O2
-
 ?

Subunits

EC Number Subunits Comment Organism
1.1.3.15 tetramer 4 * 48200, SDS-PAGE Aerococcus viridans

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.3.15 7
-
-
 Aerococcus viridans

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.3.15 FMN
-
 Aerococcus viridans

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1.1.3.15 0.00169
-
 Cibacron blue 3GA at pH 6.0 Aerococcus viridans
1.1.3.15 0.3
-
 L-Mandelate at pH 7.0 Aerococcus viridans
1.1.3.15 0.317
-
 glycolate at pH 7.0 Aerococcus viridans
1.1.3.15 0.317
-
 2-hydroxy butyrate at pH 7.0 Aerococcus viridans
1.1.3.15 0.5
-
 oxalate at pH 7.0 Aerococcus viridans
1.1.3.15 9.04
-
 (R)-lactate at pH 7.0 Aerococcus viridans