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Literature summary extracted from
- Refolding and purification of Cephalosporium acremonium deacetoxycephalosporin C synthetase/hydroxylase from granules of recombinant Escherichia coli (1996), Biotechnol. Appl. Biochem., 24, 109-119.
Application
| EC Number | Application | Comment | Organism |
|---|---|---|---|
| 1.14.11.26 | synthesis | expression in Escherichia coli, overexpression as an insoluble and inactive enzyme, elaboration of refolding scheme resulting in highly active and moderately stable enzyme | Acremonium chrysogenum |
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 1.14.20.1 | overexpression as an insoluble and inactive enzyme in granules of recombinant Escherichia coli | Acremonium chrysogenum |
Organic Solvent Stability
| EC Number | Organic Solvent | Comment | Organism |
|---|---|---|---|
| 1.14.20.1 | urea | purification is achieved by an anion-exchange-chromatographic step in the presence of denaturing concentrations of urea. The main obstacle to converting the homogenous unfolded protein into the active enzyme is a urea-dependent aggregation during refolding that led to irreversible enzyme inactivation | Acremonium chrysogenum |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.14.11.26 | Acremonium chrysogenum | - |
recombinant enzyme, catalyzes both reaction of EC 1.14.20.1 and of EC 1.14.11.26 | - |
| 1.14.20.1 | Acremonium chrysogenum | - |
bifunctional enzyme deacetoxycephalosporin C synthetase/hydroxylase | - |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 1.14.11.26 | expression in Escherichia coli, overexpression as an insoluble and inactive enzyme, elaboration of refolding scheme resulting in highly active and moderately stable enzyme | Acremonium chrysogenum |
| 1.14.20.1 | purification of refolding-competent recombinant enzyme | Acremonium chrysogenum |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 1.14.20.1 | 0.264 | - |
- |
Acremonium chrysogenum |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.14.20.1 | penicillin N + 2-oxoglutarate + O2 | - |
Acremonium chrysogenum | deacetoxycephalosporin C + succinate + CO2 + H2O | - |
? |  |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 1.14.20.1 | additional information | - |
at pH 10 enzyme has similar stability at 4°C and at 25°C. At pH 11 the enzyme is more stable at 4°C than at 25°C | Acremonium chrysogenum |
pH Stability
| EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|---|
| 1.14.20.1 | 10 | - |
enzyme has similar stability at 4°C and at 25°C | Acremonium chrysogenum |
| 1.14.20.1 | 11 | - |
enzyme is more stable at 4°C than at 25°C | Acremonium chrysogenum |
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