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Literature summary extracted from
- Deacetoxycephalosporin C synthase isozymes exhibit diverse catalytic activity and substrate specificity (2003), FEMS Microbiol. Lett., 218, 251-257.
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 1.14.20.1 | DNA and amino acid sequence determination and analysis, expression as GST-fusion protein in Escherichia coli strain BL21(DE3) | Streptomyces clavuligerus |
| 1.14.20.1 | DNA and amino acid sequence determination and analysis, expression as GST-fusion protein in Escherichia coli strain BL21(DE3) | Streptomyces jumonjinensis |
| 1.14.20.1 | DNA and amino acid sequence determination and analysis, expression of the wild-type enzyme and a C-terminally truncated mutant enzyme lacking 20 amino acids as GST-fusion proteins in Escherichia coli strain BL21(DE3) | Acremonium chrysogenum |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.14.20.1 | Iron | dependent on | Streptomyces clavuligerus |  |
| 1.14.20.1 | Iron | dependent on | Streptomyces jumonjinensis | |
| 1.14.20.1 | Iron | dependent on | Acremonium chrysogenum | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 1.14.20.1 | 60000 | - |
x * 60000, SDS-PAGE | Streptomyces clavuligerus |
| 1.14.20.1 | 60000 | - |
x * 60000, SDS-PAGE | Streptomyces jumonjinensis |
| 1.14.20.1 | 60000 | - |
x * 60000, recombinant enzyme, SDS-PAGE | Streptomyces clavuligerus |
| 1.14.20.1 | 60000 | - |
x * 60000, recombinant enzyme, SDS-PAGE | Streptomyces jumonjinensis |
| 1.14.20.1 | 60000 | - |
x * 60000, recombinant truncated mutant enzyme, SDS-PAGE, x * 62000, recombinant wild-type enzyme | Acremonium chrysogenum |
| 1.14.20.1 | 62000 | - |
x * 60000, recombinant truncated mutant enzyme, SDS-PAGE, x * 62000, recombinant wild-type enzyme | Acremonium chrysogenum |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.14.20.1 | deacetoxycephalosporin C + 2-oxoglutarate + O2 | Acremonium chrysogenum | - |
deacetylcephalosporin C + succinate + CO2 | - |
ir | |
| 1.14.20.1 | additional information | Acremonium chrysogenum | the enzyme catalyzes the first and second step in the cephamycin C biosynthetic pathway | ? | - |
? | |
| 1.14.20.1 | penicillin N + 2-oxoglutarate + O2 | Acremonium chrysogenum | - |
deacetoxycephalosporin C + succinate + CO2 + H2O | - |
ir | |
| 1.14.20.1 | penicillin N + 2-oxoglutarate + O2 | Streptomyces clavuligerus | first step in cephamycin C biosynthetic pathway | deacetoxycephalosporin C + succinate + CO2 + H2O | - |
ir | |
| 1.14.20.1 | penicillin N + 2-oxoglutarate + O2 | Streptomyces jumonjinensis | first step in cephamycin C biosynthetic pathway | deacetoxycephalosporin C + succinate + CO2 + H2O | - |
ir | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.14.20.1 | Acremonium chrysogenum | - |
- |
- |
| 1.14.20.1 | Acremonium chrysogenum | Q9P4T5 | bifunctional enzyme | - |
| 1.14.20.1 | Streptomyces clavuligerus | - |
- |
- |
| 1.14.20.1 | Streptomyces jumonjinensis | - |
- |
- |
| 1.14.20.1 | Streptomyces jumonjinensis | Q93FD4 | - |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 1.14.20.1 | recombinant enzyme from Escherichia coli strain BL21(DE3) by affinity chromatography | Streptomyces clavuligerus |
| 1.14.20.1 | recombinant enzyme from Escherichia coli strain BL21(DE3) by affinity chromatography | Streptomyces jumonjinensis |
| 1.14.20.1 | recombinant enzyme from Escherichia coli strain BL21(DE3) by affinity chromatography | Acremonium chrysogenum |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 1.14.20.1 | 0.013 | - |
recombinant enzyme, substrate amoxicillin | Streptomyces jumonjinensis |
| 1.14.20.1 | 0.014 | - |
purified recombinant wild-type and truncated mutant enzyme, substrate metampicillin | Acremonium chrysogenum |
| 1.14.20.1 | 0.015 | - |
recombinant enzyme, substrate metampicillin | Streptomyces clavuligerus |
| 1.14.20.1 | 0.023 | - |
purified recombinant truncated mutant enzyme, substrate carbenicillin | Acremonium chrysogenum |
| 1.14.20.1 | 0.026 | - |
purified recombinant wild-type enzyme, substrate carbenicillin | Acremonium chrysogenum |
| 1.14.20.1 | 0.026 | - |
recombinant enzyme, substrate metampicillin | Streptomyces jumonjinensis |
| 1.14.20.1 | 0.037 | - |
recombinant enzyme, substrate carbenicillin | Streptomyces clavuligerus |
| 1.14.20.1 | 0.039 | - |
purified recombinant wild-type and truncated mutant enzyme, substrate ampicillin, determined by bioassay | Acremonium chrysogenum |
| 1.14.20.1 | 0.051 | - |
recombinant enzyme, substrate penicillin V | Streptomyces jumonjinensis |
| 1.14.20.1 | 0.071 | - |
purified recombinant truncated mutant enzyme, substrate phenethicillin | Acremonium chrysogenum |
| 1.14.20.1 | 0.074 | - |
purified recombinant wild-type enzyme, substrate phenethicillin | Acremonium chrysogenum |
| 1.14.20.1 | 0.128 | - |
recombinant enzyme, substrate carbenicillin | Streptomyces jumonjinensis |
| 1.14.20.1 | 0.133 | - |
purified recombinant wild-type enzyme, substrate ampicillin, determined by HPLC | Acremonium chrysogenum |
| 1.14.20.1 | 0.178 | - |
purified recombinant truncated mutant enzyme, substrate ampicillin, determined by HPLC | Acremonium chrysogenum |
| 1.14.20.1 | 0.203 | - |
recombinant enzyme, substrate ampicillin, determined by bioassay | Streptomyces clavuligerus |
| 1.14.20.1 | 0.237 | - |
purified recombinant truncated mutant enzyme, substrate penicillin G | Acremonium chrysogenum |
| 1.14.20.1 | 0.24 | - |
purified recombinant wild-type enzyme, substrate penicillin G | Acremonium chrysogenum |
| 1.14.20.1 | 0.36 | - |
recombinant enzyme, substrate penicillin G | Streptomyces clavuligerus |
| 1.14.20.1 | 0.411 | - |
recombinant enzyme, substrate phenethicillin | Streptomyces clavuligerus |
| 1.14.20.1 | 0.458 | - |
recombinant enzyme, substrate ampicillin, determined by bioassay | Streptomyces jumonjinensis |
| 1.14.20.1 | 0.932 | - |
recombinant enzyme, substrate ampicillin | Streptomyces jumonjinensis |
| 1.14.20.1 | 0.932 | - |
recombinant enzyme, substrate ampicillin, determined by HPLC | Streptomyces jumonjinensis |
| 1.14.20.1 | 1.042 | - |
recombinant enzyme, substrate penicillin G | Streptomyces jumonjinensis |
| 1.14.20.1 | 1.551 | - |
recombinant enzyme, substrate phenethicillin | Streptomyces jumonjinensis |
| 1.14.20.1 | 2.221 | - |
recombinant enzyme, substrate ampicillin, determined by HPLC | Streptomyces clavuligerus |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.14.20.1 | amoxicillin + 2-oxoglutarate + O2 | - |
Streptomyces clavuligerus | ? + succinate + CO2 + H2O | - |
? | |
| 1.14.20.1 | amoxicillin + 2-oxoglutarate + O2 | - |
Streptomyces jumonjinensis | ? | - |
ir | |
| 1.14.20.1 | ampicillin + 2-oxoglutarate + O2 | - |
Streptomyces clavuligerus | cephalexin + succinate + CO2 + H2O | - |
? | |
| 1.14.20.1 | ampicillin + 2-oxoglutarate + O2 | - |
Streptomyces jumonjinensis | cephalexin + succinate + CO2 + H2O | - |
? | |
| 1.14.20.1 | ampicillin + 2-oxoglutarate + O2 | - |
Streptomyces clavuligerus | ? | - |
ir | |
| 1.14.20.1 | ampicillin + 2-oxoglutarate + O2 | - |
Streptomyces jumonjinensis | ? | - |
ir | |
| 1.14.20.1 | ampicillin + 2-oxoglutarate + O2 | - |
Acremonium chrysogenum | ? | - |
ir | |
| 1.14.20.1 | carbenicillin + 2-oxoglutarate + O2 | - |
Streptomyces clavuligerus | ? + succinate + CO2 + H2O | - |
? | |
| 1.14.20.1 | carbenicillin + 2-oxoglutarate + O2 | - |
Streptomyces jumonjinensis | ? + succinate + CO2 + H2O | - |
? | |
| 1.14.20.1 | carbenicillin + 2-oxoglutarate + O2 | - |
Streptomyces clavuligerus | ? | - |
ir | |
| 1.14.20.1 | carbenicillin + 2-oxoglutarate + O2 | - |
Streptomyces jumonjinensis | ? | - |
ir | |
| 1.14.20.1 | carbenicillin + 2-oxoglutarate + O2 | - |
Acremonium chrysogenum | ? | - |
ir | |
| 1.14.20.1 | deacetoxycephalosporin C + 2-oxoglutarate + O2 | - |
Acremonium chrysogenum | deacetylcephalosporin C + succinate + CO2 | - |
ir | |
| 1.14.20.1 | metampicillin + 2-oxoglutarate + O2 | - |
Streptomyces clavuligerus | ? + succinate + CO2 + H2O | - |
? | |
| 1.14.20.1 | metampicillin + 2-oxoglutarate + O2 | - |
Streptomyces jumonjinensis | ? + succinate + CO2 + H2O | - |
? | |
| 1.14.20.1 | metampicillin + 2-oxoglutarate + O2 | - |
Streptomyces clavuligerus | ? | - |
ir | |
| 1.14.20.1 | metampicillin + 2-oxoglutarate + O2 | - |
Streptomyces jumonjinensis | ? | - |
ir | |
| 1.14.20.1 | metampicillin + 2-oxoglutarate + O2 | best substrate | Acremonium chrysogenum | ? | - |
ir | |
| 1.14.20.1 | additional information | the enzyme catalyzes the first and second step in the cephamycin C biosynthetic pathway | Acremonium chrysogenum | ? | - |
? | |
| 1.14.20.1 | additional information | substrate specificity, amoxicillin, penicillin V, and oxacillin are no substrate | Streptomyces clavuligerus | ? | - |
? | |
| 1.14.20.1 | additional information | substrate specificity, oxacillin is no substrate | Streptomyces jumonjinensis | ? | - |
? | |
| 1.14.20.1 | additional information | substrate specificity, oxacillin, penicillin V, and amoxicillin are no substrate | Acremonium chrysogenum | ? | - |
? | |
| 1.14.20.1 | penicillin G + 2-oxoglutarate + O2 | - |
Streptomyces clavuligerus | phenylacetyl-7-aminodeacetoxy cephalosporanic acid + succinate + CO2 + H2O | - |
? | |
| 1.14.20.1 | penicillin G + 2-oxoglutarate + O2 | - |
Streptomyces jumonjinensis | phenylacetyl-7-aminodeacetoxy cephalosporanic acid + succinate + CO2 + H2O | - |
? | |
| 1.14.20.1 | penicillin G + 2-oxoglutarate + O2 | - |
Streptomyces clavuligerus | phenylacetyl-7-aminodeacetoxycephalosporanic acid + succinate + CO2 + H2O | - |
ir | |
| 1.14.20.1 | penicillin G + 2-oxoglutarate + O2 | - |
Streptomyces jumonjinensis | phenylacetyl-7-aminodeacetoxycephalosporanic acid + succinate + CO2 + H2O | - |
ir | |
| 1.14.20.1 | penicillin G + 2-oxoglutarate + O2 | - |
Acremonium chrysogenum | phenylacetyl-7-aminodeacetoxycephalosporanic acid + succinate + CO2 + H2O | - |
ir | |
| 1.14.20.1 | penicillin N + 2-oxoglutarate + O2 | - |
Acremonium chrysogenum | deacetoxycephalosporin C + succinate + CO2 + H2O | - |
? | |
| 1.14.20.1 | penicillin N + 2-oxoglutarate + O2 | - |
Streptomyces clavuligerus | deacetoxycephalosporin C + succinate + CO2 + H2O | - |
? | |
| 1.14.20.1 | penicillin N + 2-oxoglutarate + O2 | - |
Streptomyces clavuligerus | deacetoxycephalosporin C + succinate + CO2 + H2O | - |
ir | |
| 1.14.20.1 | penicillin N + 2-oxoglutarate + O2 | - |
Streptomyces jumonjinensis | deacetoxycephalosporin C + succinate + CO2 + H2O | - |
? | |
| 1.14.20.1 | penicillin N + 2-oxoglutarate + O2 | - |
Streptomyces jumonjinensis | deacetoxycephalosporin C + succinate + CO2 + H2O | - |
ir | |
| 1.14.20.1 | penicillin N + 2-oxoglutarate + O2 | - |
Acremonium chrysogenum | deacetoxycephalosporin C + succinate + CO2 + H2O | - |
ir | |
| 1.14.20.1 | penicillin N + 2-oxoglutarate + O2 | first step in cephamycin C biosynthetic pathway | Streptomyces clavuligerus | deacetoxycephalosporin C + succinate + CO2 + H2O | - |
ir | |
| 1.14.20.1 | penicillin N + 2-oxoglutarate + O2 | first step in cephamycin C biosynthetic pathway | Streptomyces jumonjinensis | deacetoxycephalosporin C + succinate + CO2 + H2O | - |
ir | |
| 1.14.20.1 | penicillin V + 2-oxoglutarate + O2 | - |
Streptomyces clavuligerus | ? + succinate + CO2 + H2O | - |
? | |
| 1.14.20.1 | penicillin V + 2-oxoglutarate + O2 | - |
Streptomyces jumonjinensis | ? | - |
ir | |
| 1.14.20.1 | phenethicillin + 2-oxoglutarate + O2 | - |
Streptomyces clavuligerus | ? + succinate + CO2 + H2O | - |
? | |
| 1.14.20.1 | phenethicillin + 2-oxoglutarate + O2 | - |
Streptomyces jumonjinensis | ? + succinate + CO2 + H2O | - |
? | |
| 1.14.20.1 | phenethicillin + 2-oxoglutarate + O2 | - |
Streptomyces jumonjinensis | ? | - |
ir | |
| 1.14.20.1 | phenethicillin + 2-oxoglutarate + O2 | - |
Acremonium chrysogenum | ? | - |
ir | |
| 1.14.20.1 | phenethicillin + 2-oxoglutarate + O2 | best substrate | Streptomyces clavuligerus | ? | - |
ir | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 1.14.20.1 | ? | x * 60000, SDS-PAGE | Streptomyces clavuligerus |
| 1.14.20.1 | ? | x * 60000, SDS-PAGE | Streptomyces jumonjinensis |
| 1.14.20.1 | ? | x * 60000, recombinant enzyme, SDS-PAGE | Streptomyces clavuligerus |
| 1.14.20.1 | ? | x * 60000, recombinant enzyme, SDS-PAGE | Streptomyces jumonjinensis |
| 1.14.20.1 | ? | x * 60000, recombinant truncated mutant enzyme, SDS-PAGE, x * 62000, recombinant wild-type enzyme | Acremonium chrysogenum |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 1.14.20.1 | DAOC/DACS | - |
Acremonium chrysogenum |
| 1.14.20.1 | DAOCS | - |
Streptomyces clavuligerus |
| 1.14.20.1 | DAOCS | - |
Streptomyces jumonjinensis |
| 1.14.20.1 | deacetoxy/deacetylcephalosporin C synthase | - |
Acremonium chrysogenum |
| 1.14.20.1 | deacetoxycephalosporin C synthase | - |
Streptomyces clavuligerus |
| 1.14.20.1 | deacetoxycephalosporin C synthase | - |
Streptomyces jumonjinensis |
| 1.14.20.1 | expandase | - |
Streptomyces clavuligerus |
| 1.14.20.1 | expandase | - |
Streptomyces jumonjinensis |
| 1.14.20.1 | expandase | - |
Acremonium chrysogenum |
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