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Literature summary extracted from

  • Martin, R.C.; Mok, M.C.; Shaw, G.; Mok, D.W.
    An enzyme mediating the conversion of zeatin to dihydrozeatin in Phaseolus embryos (1989), Plant Physiol., 90, 1630-1635.
    View publication on PubMedView publication on EuropePMC

General Stability

EC Number General Stability Organism
1.3.1.69 glycerol, 20%, stabilizes Phaseolus vulgaris

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.3.1.69 0.07
-
 trans-zeatin high molecular weight isoenzyme Phaseolus vulgaris
1.3.1.69 0.1 0.23 trans-zeatin low molecular weight isoenzyme, instability of the isoenzyme after gel filtration may contibute to the variation between experiments Phaseolus vulgaris

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.3.1.69 soluble
-
 Phaseolus vulgaris
-
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.3.1.69 25000
-
 low molecular weight isoenzyme, gel filtration Phaseolus vulgaris
1.3.1.69 55000
-
 high molecular weight isoenzyme, gel filtration Phaseolus vulgaris

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.3.1.69 trans-zeatin + NADPH Phaseolus vulgaris
-
 dihydrozeatin + NADP+
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.3.1.69 Phaseolus vulgaris
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.3.1.69 partial Phaseolus vulgaris

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.3.1.69 embryo immature Phaseolus vulgaris
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.3.1.69 additional information
-
-
 Phaseolus vulgaris

Storage Stability

EC Number Storage Stability Organism
1.3.1.69 -20°C, in 0.05 M phosphate buffer containing 5 mM DTT, 0.5 mM EDTA, 5 mM NADPH, 0.5 M KCl and 20% glycerol: loss of about 10% of activity after 3 days Phaseolus vulgaris

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.3.1.69 additional information no activity with: ribosylzeatin, cis-zeatin, O-xylosylzeatin, N6-(DELTA2-isopentenyl)adenine, or N6-(DELTA2-isotentenyl)adenosine Phaseolus vulgaris ?
-
 ?
1.3.1.69 trans-zeatin + NADPH
-
 Phaseolus vulgaris dihydrozeatin + NADP+
-
 ?
1.3.1.69 zeatin + NADPH
-
 Phaseolus vulgaris dihydrozeatin + NADP+
-
 ?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.3.1.69 7.5 8
-
 Phaseolus vulgaris

Cofactor

EC Number Cofactor Comment Organism Structure
1.3.1.69 NADPH required, NADH cannot substitute for NADPH Phaseolus vulgaris