Literature summary extracted from
Jiang, H.; Parales, R.E.; Lynch, N.A.; Gibson, D.T.
Site-directed mutagenesis of conserved amino acids in the alpha subunit of toluene dioxygenase: potential mononuclear non-heme iron coordination sites (1996), J. Bacteriol., 178, 3133-3139.
Protein Variants
EC Number |
Protein Variants |
Comment |
Organism |
---|
1.14.12.11 |
D219A |
mutation at alpha-subunit of oxygenase component completely abolishes toluene dioxygenase activity, mutation completely eliminates formation of cis-toluene dihydrodiol |
Pseudomonas putida |
1.14.12.11 |
E214A |
mutation at alpha-subunit of oxygenase component completely abolishes toluene dioxygenase activity, mutation completely eliminates formation of cis-toluene dihydrodiol |
Pseudomonas putida |
1.14.12.11 |
H222A |
mutation at alpha-subunit of oxygenase component completely abolishes toluene dioxygenase activity, mutation completely eliminates formation of cis-toluene dihydrodiol |
Pseudomonas putida |
1.14.12.11 |
H228A |
mutation at alpha-subunit of oxygenase component completely abolishes toluene dioxygenase activity, mutation completely eliminates formation of cis-toluene dihydrodiol |
Pseudomonas putida |
1.14.12.11 |
Y221A |
mutation at alpha-subunit of oxygenase component, 42% of the activity of the wild-type enzyme, formation of cis-toluene dihydrodiol is reduced |
Pseudomonas putida |
1.14.12.11 |
Y266A |
mutation at alpha-subunit of oxygenase component, 12% of the activity of the wild-type enzyme, formation of cis-toluene dihydrodiol is reduced |
Pseudomonas putida |
Metals/Ions
EC Number |
Metals/Ions |
Comment |
Organism |
Structure |
---|
1.14.12.11 |
Iron |
enzyme requires mononuclear iron for activity |
Pseudomonas putida |
|
Organism
EC Number |
Organism |
UniProt |
Comment |
Textmining |
---|
1.14.12.11 |
Pseudomonas putida |
- |
F1 |
- |
Purification (Commentary)
EC Number |
Purification (Comment) |
Organism |
---|
1.14.12.11 |
beta-subunit of oxygenase component |
Pseudomonas putida |
Substrates and Products (Substrate)
EC Number |
Substrates |
Comment Substrates |
Organism |
Products |
Comment (Products) |
Rev. |
Reac. |
---|
1.14.12.11 |
toluene + NADH + O2 |
- |
Pseudomonas putida |
(1S,2R)-3-methylcyclohexa-3,5-diene-1,2-diol + NAD+ |
- |
? |
|
Cofactor
EC Number |
Cofactor |
Comment |
Organism |
Structure |
---|
1.14.12.11 |
NADH |
- |
Pseudomonas putida |
|