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Literature summary extracted from

  • Kreisberg-Zakarin, R.; Borovok, I.; Yanko, M.; Frolow, F.; Aharonowitz, Y.; Cohen, G.
    Structure-function studies of the non-heme iron active site of isopenicillin N synthase: some implications for catalysis (2000), Biophys. Chem., 86, 109-118.
    View publication on PubMed

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
1.21.3.1 crystal structure, molecular modeling of the active site structure and the Fe2+-binding motif Streptomyces jumonjinensis

Protein Variants

EC Number Protein Variants Comment Organism
1.21.3.1 D214C site-directed mutagenesis, active site mutant, complete loss of activity Streptomyces jumonjinensis
1.21.3.1 D214E site-directed mutagenesis, active site mutant, retains 1% of activity compared to wild-type Streptomyces jumonjinensis
1.21.3.1 D214H site-directed mutagenesis, active site mutant, complete loss of activity Streptomyces jumonjinensis
1.21.3.1 H212D site-directed mutagenesis, active site mutant, complete loss of activity Streptomyces jumonjinensis
1.21.3.1 H212N site-directed mutagenesis, active site mutant, complete loss of activity Streptomyces jumonjinensis
1.21.3.1 H212Q site-directed mutagenesis, active site mutant, complete loss of activity Streptomyces jumonjinensis
1.21.3.1 H268D site-directed mutagenesis, active site mutant, complete loss of activity Streptomyces jumonjinensis
1.21.3.1 H268N site-directed mutagenesis, active site mutant, complete loss of activity Streptomyces jumonjinensis
1.21.3.1 H268Q site-directed mutagenesis, active site mutant, complete loss of activity Streptomyces jumonjinensis
1.21.3.1 additional information exchange of Asp214 and His268, and exchange of Asp214 and His 212 by site-directed mutagenesis leads to completely inactive enzymes Streptomyces jumonjinensis

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.21.3.1 Fe2+ iron-binding motif Streptomyces jumonjinensis
1.21.3.1 Fe2+ structure of the Fe2+ active site and endogenous ligands Streptomyces jumonjinensis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.21.3.1 N-[(5S)-5-amino-5-carboxypentanoyl]-L-cysteinyl-D-valine + O2 Streptomyces jumonjinensis
-
 isopenicillin N + 2 H2O
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.21.3.1 Streptomyces jumonjinensis
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.21.3.1 N-[(5S)-5-amino-5-carboxypentanoyl]-L-cysteinyl-D-valine + O2
-
 Streptomyces jumonjinensis isopenicillin N + 2 H2O
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.21.3.1 IPNS
-
 Streptomyces jumonjinensis