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Literature summary extracted from

  • Dubus, A.; Sami, M.; Brown, T.J.N.; Schofield, C.J.; Baldwin, J.E.; Frere, J.M.
    Studies of isopenicillin N synthase enzymatic properties using a continuous spectrophotometric assay (2000), FEBS Lett., 485, 142-146.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.21.3.1 ascorbic acid
-
 Aspergillus nidulans

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.21.3.1 0.12
-
 N-[(5S)-5-amino-5-carboxypentanoyl]-L-cysteinyl-D-valine
-
 Aspergillus nidulans

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.21.3.1 Fe2+
-
 Aspergillus nidulans

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.21.3.1 N-[(5S)-5-amino-5-carboxypentanoyl]-L-cysteinyl-D-valine + O2 Aspergillus nidulans common step in the biosynthesis of penicillins, cephalosporins and cephamycins isopenicillin N + 2 H2O
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.21.3.1 Aspergillus nidulans
-
-
-

Oxidation Stability

EC Number Oxidation Stability Organism
1.21.3.1 enzyme is unstable to oxidizing oxygen species in the reaction solution Aspergillus nidulans

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.21.3.1 additional information
-
 development of spectrophotometric assay Aspergillus nidulans
1.21.3.1 0.00000664
-
 purified recombinant enzyme Aspergillus nidulans

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.21.3.1 N-[(5S)-5-amino-5-carboxypentanoyl]-L-cysteinyl-D-valine + O2
-
 Aspergillus nidulans isopenicillin N + 2 H2O
-
 ?
1.21.3.1 N-[(5S)-5-amino-5-carboxypentanoyl]-L-cysteinyl-D-valine + O2 common step in the biosynthesis of penicillins, cephalosporins and cephamycins Aspergillus nidulans isopenicillin N + 2 H2O
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.21.3.1 IPNS
-
 Aspergillus nidulans

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.21.3.1 30
-
 assay Aspergillus nidulans

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.21.3.1 4.1
-
 N-[(5S)-5-amino-5-carboxypentanoyl]-L-cysteinyl-D-valine
-
 Aspergillus nidulans

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.21.3.1 7
-
 assay at Aspergillus nidulans