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Literature summary extracted from

  • Loke, P.; Ng, C.P.; Sim, T.S.
    PCR cloning, heterologous expression, and characterization of isopenicillin N synthase from Streptomyces lipmanii NRRL 3584 (2000), Can. J. Microbiol., 46, 166-170.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.21.3.1 overexpression in Escherichia coli, DNA sequence analysis Streptomyces microflavus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.21.3.1 37800
-
 x * 37800, DNA sequence determination Streptomyces microflavus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.21.3.1 N-[(5S)-5-amino-5-carboxypentanoyl]-L-cysteinyl-D-valine + O2 Streptomyces microflavus common step in the biosynthesis of penicillins, cephalosporins and cephamycins isopenicillin N + 2 H2O
-
 ?
1.21.3.1 N-[(5S)-5-amino-5-carboxypentanoyl]-L-cysteinyl-D-valine + O2 Streptomyces microflavus NRRL 3584 common step in the biosynthesis of penicillins, cephalosporins and cephamycins isopenicillin N + 2 H2O
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.21.3.1 Streptomyces microflavus
-
-
-
1.21.3.1 Streptomyces microflavus NRRL 3584
-
-
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.21.3.1 0.000000093
-
 purified recombinant enzyme Streptomyces microflavus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.21.3.1 N-[(5S)-5-amino-5-carboxypentanoyl]-L-cysteinyl-D-valine + O2
-
 Streptomyces microflavus isopenicillin N + 2 H2O
-
 ?
1.21.3.1 N-[(5S)-5-amino-5-carboxypentanoyl]-L-cysteinyl-D-valine + O2 common step in the biosynthesis of penicillins, cephalosporins and cephamycins Streptomyces microflavus isopenicillin N + 2 H2O
-
 ?
1.21.3.1 N-[(5S)-5-amino-5-carboxypentanoyl]-L-cysteinyl-D-valine + O2
-
 Streptomyces microflavus NRRL 3584 isopenicillin N + 2 H2O
-
 ?
1.21.3.1 N-[(5S)-5-amino-5-carboxypentanoyl]-L-cysteinyl-D-valine + O2 common step in the biosynthesis of penicillins, cephalosporins and cephamycins Streptomyces microflavus NRRL 3584 isopenicillin N + 2 H2O
-
 ?

Subunits

EC Number Subunits Comment Organism
1.21.3.1 ? x * 37800, DNA sequence determination Streptomyces microflavus

Synonyms

EC Number Synonyms Comment Organism
1.21.3.1 IPNS
-
 Streptomyces microflavus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.21.3.1 26
-
 assay at Streptomyces microflavus