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Literature summary extracted from

  • Katano, N.; Yamamoto, H.; Iio, R.; Inoue, K.
    7-Deoxyloganin 7-hydroxylase in Lonicera japonica cell cultures (2001), Phytochemistry, 58, 53-58.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.14.14.85 Ancymidol 0.01 mM, 58.4% inhibition, 0.1 mM, 77.0% inhibition Lonicera japonica
1.14.14.85 CO 60% inhibition in the dark, partially reversible by light Lonicera japonica
1.14.14.85 cytochrome c 0.01 mM, 44.9% inhibition, 0.1 mM, 59.6% inhibition Lonicera japonica
1.14.14.85 ketoconazole 0.01 mM, 93% inhibition, 0.1 mM, complete inhibition Lonicera japonica
1.14.14.85 Metyrapone 0.01 mM, 73.6% inhibition, 0.1 mM, 92.4% inhibition Lonicera japonica
1.14.14.85 miconazole 0.01 mM, 26.2% inhibition, 0.1 mM, 78.5% inhibition Lonicera japonica

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.14.14.85 0.17
-
 7-deoxyloganin
-
 Lonicera japonica

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.14.14.85 microsome
-
 Lonicera japonica
-
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.14.14.85 7-deoxyloganin + [reduced NADPH-hemoprotein reductase] + O2 Lonicera japonica enzyme is involved in loganin biosynthesis loganin + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.14.14.85 Lonicera japonica
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.14.14.85 cell culture
-
 Lonicera japonica
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.14.14.85 0.000099
-
 activity in cell extracts, cofactor NADPH Lonicera japonica

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.14.85 7-deoxyloganic acid + [reduced NADPH-hemoprotein reductase] + O2 50% of activity with 7-deoxyloganin Lonicera japonica loganic acid + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?
1.14.14.85 7-deoxyloganin + [reduced NADPH-hemoprotein reductase] + O2 NADPH is essential for activity, 45% activity with NADH, substitution of O2 with N2 reduces activity by 50% Lonicera japonica loganin + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?
1.14.14.85 7-deoxyloganin + [reduced NADPH-hemoprotein reductase] + O2 enzyme is involved in loganin biosynthesis Lonicera japonica loganin + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.14.14.85 7.5
-
 50% of maximal activity at pH 7.1 and pH 9.4 Lonicera japonica