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Literature summary extracted from

  • Faber, B.W.; van Gorcom, R.F.; Duine, J.A.
    Purification and characterization of benzoate-para-hydroxylase, a cytochrome P450 (CYP53A1), from Aspergillus niger (2001), Arch. Biochem. Biophys., 394, 245-254.
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.14.14.92 0.083
-
 benzoate
-
 Aspergillus niger
1.14.14.92 0.086
-
 3-Fluorobenzoate
-
 Aspergillus niger
1.14.14.92 0.097
-
 2-Fluorobenzoate
-
 Aspergillus niger
1.14.14.92 0.127
-
 3-chlorobenzoate
-
 Aspergillus niger
1.14.14.92 0.189
-
 3-Methoxybenzoate
-
 Aspergillus niger
1.14.14.92 0.28
-
 2-Hydroxybenzoate
-
 Aspergillus niger
1.14.14.92 0.31
-
 2-Chlorobenzoate
-
 Aspergillus niger
1.14.14.92 0.673
-
 3-methylbenzoate
-
 Aspergillus niger
1.14.14.92 1.6
-
 2-Methylbenzoate
-
 Aspergillus niger

Organism

EC Number Organism UniProt Comment Textmining
1.14.14.92 Aspergillus niger
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.14.14.92 DEAE-Sepharose, benzoate-agarose Aspergillus niger

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.14.92 2-chlorobenzoate + [reduced NADPH-hemoprotein reductase] + O2
-
 Aspergillus niger 2-chloro-4-hydroxybenzoate + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?
1.14.14.92 2-fluorobenzoate + [reduced NADPH-hemoprotein reductase] + O2
-
 Aspergillus niger 2-fluoro-4-hydroxybenzoate + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?
1.14.14.92 2-hydroxybenzoate + [reduced NADPH-hemoprotein reductase] + O2
-
 Aspergillus niger 2,4-dihydroxybenzoate + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?
1.14.14.92 2-methylbenzoate + [reduced NADPH-hemoprotein reductase] + O2
-
 Aspergillus niger 2-methyl-4-hydroxybenzoate + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?
1.14.14.92 3-chlorobenzoate + [reduced NADPH-hemoprotein reductase] + O2
-
 Aspergillus niger 3-chloro-4-hydroxybenzoate + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?
1.14.14.92 3-fluorobenzoate + [reduced NADPH-hemoprotein reductase] + O2
-
 Aspergillus niger 3-fluoro-4-hydroxybenzoate + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?
1.14.14.92 3-hydroxybenzoate + [reduced NADPH-hemoprotein reductase] + O2
-
 Aspergillus niger 3,4-dihydroxybenzoate + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?
1.14.14.92 3-methoxybenzoate + [reduced NADPH-hemoprotein reductase] + O2
-
 Aspergillus niger 3-methoxy-4-hydroxybenzoate + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?
1.14.14.92 3-methylbenzoate + [reduced NADPH-hemoprotein reductase] + O2
-
 Aspergillus niger 3-methyl-4-hydroxybenzoate + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?
1.14.14.92 benzoate + [reduced NADPH-hemoprotein reductase] + O2
-
 Aspergillus niger 4-hydroxybenzoate + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?
1.14.14.92 nicotinate + [reduced NADPH-hemoprotein reductase] + O2
-
 Aspergillus niger ?
-
 ?
1.14.14.92 picolinate + [reduced NADPH-hemoprotein reductase] + O2
-
 Aspergillus niger ?
-
 ?

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.14.14.92 2.33
-
 2-Chlorobenzoate
-
 Aspergillus niger
1.14.14.92 3.67
-
 2-Methylbenzoate
-
 Aspergillus niger
1.14.14.92 3.83
-
 picolinate
-
 Aspergillus niger
1.14.14.92 4
-
 nicotinate
-
 Aspergillus niger
1.14.14.92 4
-
 3-Methoxybenzoate
-
 Aspergillus niger
1.14.14.92 4
-
 benzoate turnover rate in microsomes Aspergillus niger
1.14.14.92 4.17
-
 2-Hydroxybenzoate
-
 Aspergillus niger
1.14.14.92 4.33
-
 2-Fluorobenzoate
-
 Aspergillus niger
1.14.14.92 4.5
-
 benzoate
-
 Aspergillus niger
1.14.14.92 4.5
-
 3-hydroxybenzoate
-
 Aspergillus niger
1.14.14.92 4.83
-
 3-Fluorobenzoate
-
 Aspergillus niger
1.14.14.92 6.17
-
 3-chlorobenzoate
-
 Aspergillus niger
1.14.14.92 6.17
-
 3-methylbenzoate
-
 Aspergillus niger