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Literature summary extracted from

  • Shimomura, O.; Johnson, F.H.; Kohama, Y.
    Reactions involved in bioluminescence systems of limpet (Latia neritoides) and luminous bacteria (1972), Proc. Natl. Acad. Sci. USA, 69, 2086-2089.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.14.99.21 dithiothreitol strong increase of activity Latia neritoides
1.14.99.21 Purple protein requirement Latia neritoides

Organism

EC Number Organism UniProt Comment Textmining
1.14.99.21 Latia neritoides
-
 freshwater limpet
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.14.99.21
-
 Latia neritoides

Reaction

EC Number Reaction Comment Organism Reaction ID
1.14.99.21 Latia luciferin + reduced acceptor + 2 O2 = oxidized Latia luciferin + CO2 + formate + acceptor + H2O + hnu possibly two enzyme components are involved in the reaction, an oxygenase followed by a monooxygenase for the actual light emitting step Latia neritoides
a

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.99.21 (E)-2-methyl-4-(2,6,6-trimethyl-1-cyclohex-1-yl)-1-buten-1-ol-formate + NADH + O2 trivial name Latia luciferin Latia neritoides oxidized Latia luciferin + NAD+ + CO2 + H2O + formate + light
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.14.99.21 10
-
 assay at Latia neritoides

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.14.99.21 6.8
-
 assay at Latia neritoides

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.99.21 ascorbate activation, especially together with optimal NADH Latia neritoides
1.14.99.21 FMN flavoprotein, reductase component Latia neritoides
1.14.99.21 FMNH2 luciferase Latia neritoides
1.14.99.21 NADH reductase component Latia neritoides