BRENDA - Enzyme Database

Substrate binding and calmodulin binding to endothelial nitric oxide synthase coregulate its enzymic activity

Presta, A.; Liu, J.; Sessa, W.C.; Stuehr, D.J.; Nitric Oxide 1, 74-87 (1997)

Data extracted from this reference:

Activating Compound
EC Number
Activating Compound
Commentary
Organism
Structure
1.14.13.39
dithiothreitol
-
Bos taurus
Cloned(Commentary)
EC Number
Commentary
Organism
1.14.13.39
expression of endothelial isoform in human embryonic kidney cells
Bos taurus
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
1.14.13.39
EDTA
inhibits at concentrations above 0.01 mM
Bos taurus
1.14.13.39
Ngamma-nitro-L-arginine methyl ester
complete inhibition
Bos taurus
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.14.13.39
0.0021
-
L-arginine
-
Bos taurus
1.14.13.39
0.019
-
Ngamma-hydroxy-L-arginine
-
Bos taurus
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
1.14.13.39
Ca2+
required
Bos taurus
1.14.13.39
Iron
heme-iron
Bos taurus
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
1.14.13.39
Bos taurus
-
endothelial isoform
-
Purification (Commentary)
EC Number
Commentary
Organism
1.14.13.39
recombinant endothelial isoform from human embryonic kidney cells
Bos taurus
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
1.14.13.39
endothelium
-
Bos taurus
-
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
1.14.13.39
0.143
-
purified recombinant enzyme
Bos taurus
1.14.13.39
0.41
-
substrate Ngamma-hydroxy-L-arginine, purified enzyme
Bos taurus
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.14.13.39
2 L-arginine + 3 NADPH + 4 O2 + 3 H+
-
440240
Bos taurus
2 L-citrulline + 2 NO + 3 NADP+ + 4 H2O
-
440240
Bos taurus
?
1.14.13.39
ferricyanide + NADPH + O2
-
440240
Bos taurus
ferrocyanide + NADP+ + H2O
-
-
-
?
1.14.13.39
Ngamma-hydroxy-L-arginine + NADPH + O2
-
440240
Bos taurus
citrulline + NADP+ + NO
-
-
-
?
1.14.13.39
oxidized cytochrome c + NADPH + O2
-
440240
Bos taurus
reduced cytochrome c + NADP+ + H2O
-
-
-
?
Subunits
EC Number
Subunits
Commentary
Organism
1.14.13.39
dimer
2 * 130000-150000, SDS-PAGE
Bos taurus
Temperature Optimum [°C]
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
1.14.13.39
25
-
assay at
Bos taurus
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.14.13.39
7.4
-
assay at
Bos taurus
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
1.14.13.39
5,6,7,8-tetrahydro-L-biopterin
-
Bos taurus
1.14.13.39
Calmodulin
enzyme-bound is required, supplemented stimulates
Bos taurus
1.14.13.39
FAD
-
Bos taurus
1.14.13.39
FMN
-
Bos taurus
Activating Compound (protein specific)
EC Number
Activating Compound
Commentary
Organism
Structure
1.14.13.39
dithiothreitol
-
Bos taurus
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
1.14.13.39
expression of endothelial isoform in human embryonic kidney cells
Bos taurus
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
1.14.13.39
5,6,7,8-tetrahydro-L-biopterin
-
Bos taurus
1.14.13.39
Calmodulin
enzyme-bound is required, supplemented stimulates
Bos taurus
1.14.13.39
FAD
-
Bos taurus
1.14.13.39
FMN
-
Bos taurus
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
1.14.13.39
EDTA
inhibits at concentrations above 0.01 mM
Bos taurus
1.14.13.39
Ngamma-nitro-L-arginine methyl ester
complete inhibition
Bos taurus
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.14.13.39
0.0021
-
L-arginine
-
Bos taurus
1.14.13.39
0.019
-
Ngamma-hydroxy-L-arginine
-
Bos taurus
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
1.14.13.39
Ca2+
required
Bos taurus
1.14.13.39
Iron
heme-iron
Bos taurus
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
1.14.13.39
recombinant endothelial isoform from human embryonic kidney cells
Bos taurus
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
1.14.13.39
endothelium
-
Bos taurus
-
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
1.14.13.39
0.143
-
purified recombinant enzyme
Bos taurus
1.14.13.39
0.41
-
substrate Ngamma-hydroxy-L-arginine, purified enzyme
Bos taurus
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.14.13.39
2 L-arginine + 3 NADPH + 4 O2 + 3 H+
-
440240
Bos taurus
2 L-citrulline + 2 NO + 3 NADP+ + 4 H2O
-
440240
Bos taurus
?
1.14.13.39
ferricyanide + NADPH + O2
-
440240
Bos taurus
ferrocyanide + NADP+ + H2O
-
-
-
?
1.14.13.39
Ngamma-hydroxy-L-arginine + NADPH + O2
-
440240
Bos taurus
citrulline + NADP+ + NO
-
-
-
?
1.14.13.39
oxidized cytochrome c + NADPH + O2
-
440240
Bos taurus
reduced cytochrome c + NADP+ + H2O
-
-
-
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
1.14.13.39
dimer
2 * 130000-150000, SDS-PAGE
Bos taurus
Temperature Optimum [°C] (protein specific)
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
1.14.13.39
25
-
assay at
Bos taurus
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.14.13.39
7.4
-
assay at
Bos taurus