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Literature summary extracted from
- Substrate binding and calmodulin binding to endothelial nitric oxide synthase coregulate its enzymic activity (1997), Nitric Oxide, 1, 74-87.
Activating Compound
| EC Number | Activating Compound | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.14.13.39 | dithiothreitol | - |
Bos taurus |  |
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 1.14.13.39 | expression of endothelial isoform in human embryonic kidney cells | Bos taurus |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.14.13.39 | EDTA | inhibits at concentrations above 0.01 mM | Bos taurus | |
| 1.14.13.39 | Ngamma-nitro-L-arginine methyl ester | complete inhibition | Bos taurus | |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.14.13.39 | 0.0021 | - |
L-arginine | - |
Bos taurus | |
| 1.14.13.39 | 0.019 | - |
Ngamma-hydroxy-L-arginine | - |
Bos taurus | |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.14.13.39 | Ca2+ | required | Bos taurus | |
| 1.14.13.39 | Iron | heme-iron | Bos taurus | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.14.13.39 | Bos taurus | - |
endothelial isoform | - |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 1.14.13.39 | recombinant endothelial isoform from human embryonic kidney cells | Bos taurus |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 1.14.13.39 | endothelium | - |
Bos taurus | - |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 1.14.13.39 | 0.143 | - |
purified recombinant enzyme | Bos taurus |
| 1.14.13.39 | 0.41 | - |
substrate Ngamma-hydroxy-L-arginine, purified enzyme | Bos taurus |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.14.13.39 | 2 L-arginine + 3 NADPH + 4 O2 + 3 H+ | - |
Bos taurus | 2 L-citrulline + 2 NO + 3 NADP+ + 4 H2O | - |
? | |
| 1.14.13.39 | ferricyanide + NADPH + O2 | - |
Bos taurus | ferrocyanide + NADP+ + ? | - |
? | |
| 1.14.13.39 | Ngamma-hydroxy-L-arginine + NADPH + O2 | - |
Bos taurus | citrulline + NADP+ + NO | - |
? | |
| 1.14.13.39 | oxidized cytochrome c + NADPH + O2 | - |
Bos taurus | reduced cytochrome c + NADP+ + H2O | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 1.14.13.39 | dimer | 2 * 130000-150000, SDS-PAGE | Bos taurus |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 1.14.13.39 | e-NOS | endothelial isoform III | Bos taurus |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 1.14.13.39 | 25 | - |
assay at | Bos taurus |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 1.14.13.39 | 7.4 | - |
assay at | Bos taurus |
Cofactor
| EC Number | Cofactor | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.14.13.39 | 5,6,7,8-tetrahydro-L-biopterin | - |
Bos taurus | |
| 1.14.13.39 | Calmodulin | enzyme-bound is required, supplemented stimulates | Bos taurus | |
| 1.14.13.39 | FAD | - |
Bos taurus | |
| 1.14.13.39 | FMN | - |
Bos taurus | |
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Release 2023.1 (January 2023)
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