BRENDA - Enzyme Database

Characterization of C415 mutants of neuronal nitric oxide synthase

Richards, M.K.; Clague, M.J.; Marletta, M.A.; Biochemistry 35, 7772-7780 (1996)

Data extracted from this reference:

Activating Compound
EC Number
Activating Compound
Commentary
Organism
Structure
1.14.13.39
dithiothreitol
-
Mus musculus
Cloned(Commentary)
EC Number
Commentary
Organism
1.14.13.39
expression of neuronal isoform wild type and mutants C415H, C415A in Spodoptera frugiperda cells via baculovirus infection
Mus musculus
Engineering
EC Number
Amino acid exchange
Commentary
Organism
1.14.13.39
C415A
contains no heme, no bound tetrahydrobiopterin, unable to oxidize NADPH and to synthezise nitric oxide, unaltered ability to reduce cytochrome c
Mus musculus
1.14.13.39
C415H
contains nearly no heme, no bound tetrahydrobiopterin, unable to oxidize NADPH and to synthezise nitric oxide, unaltered ability to reduce cytochrome c
Mus musculus
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
1.14.13.39
Ca2+
-
Mus musculus
1.14.13.39
Iron
heme ligand is bound via C415
Mus musculus
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
1.14.13.39
Mus musculus
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
1.14.13.39
wild-type and mutants from insect cells
Mus musculus
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
1.14.13.39
macrophage
-
Mus musculus
-
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [Ámol/min/mg]
Specific Activity Maximum [Ámol/min/mg]
Commentary
Organism
1.14.13.39
additional information
-
wild-type and mutants
Mus musculus
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.14.13.39
2 L-arginine + 3 NADPH + 4 O2 + 3 H+
-
440234
Mus musculus
2 L-citrulline + 2 NO + 3 NADP+ + 4 H2O
-
440234
Mus musculus
?
1.14.13.39
oxidized cytochrome c + NADPH + O2
wild-type and mutants
440234
Mus musculus
reduced cytochrome c + NADP+ + H2O
-
-
-
?
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
1.14.13.39
5,6,7,8-tetrahydro-L-biopterin
only wild-type
Mus musculus
1.14.13.39
Calmodulin
15fold stimulation of cytochrome c reduction of wild-type and mutants C415A and C415H; Ca2+/calmodulin stimulates cytochrome c reductase activity
Mus musculus
1.14.13.39
FAD
wild-type and mutant C415H contain1 mol per mol of subunit
Mus musculus
1.14.13.39
FMN
wild-type and mutant C415H contain 0.8 and 0.9 mol per mol of subunit, respectively
Mus musculus
1.14.13.39
NADPH
-
Mus musculus
Activating Compound (protein specific)
EC Number
Activating Compound
Commentary
Organism
Structure
1.14.13.39
dithiothreitol
-
Mus musculus
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
1.14.13.39
expression of neuronal isoform wild type and mutants C415H, C415A in Spodoptera frugiperda cells via baculovirus infection
Mus musculus
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
1.14.13.39
5,6,7,8-tetrahydro-L-biopterin
only wild-type
Mus musculus
1.14.13.39
Calmodulin
15fold stimulation of cytochrome c reduction of wild-type and mutants C415A and C415H; Ca2+/calmodulin stimulates cytochrome c reductase activity
Mus musculus
1.14.13.39
FAD
wild-type and mutant C415H contain1 mol per mol of subunit
Mus musculus
1.14.13.39
FMN
wild-type and mutant C415H contain 0.8 and 0.9 mol per mol of subunit, respectively
Mus musculus
1.14.13.39
NADPH
-
Mus musculus
Engineering (protein specific)
EC Number
Amino acid exchange
Commentary
Organism
1.14.13.39
C415A
contains no heme, no bound tetrahydrobiopterin, unable to oxidize NADPH and to synthezise nitric oxide, unaltered ability to reduce cytochrome c
Mus musculus
1.14.13.39
C415H
contains nearly no heme, no bound tetrahydrobiopterin, unable to oxidize NADPH and to synthezise nitric oxide, unaltered ability to reduce cytochrome c
Mus musculus
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
1.14.13.39
Ca2+
-
Mus musculus
1.14.13.39
Iron
heme ligand is bound via C415
Mus musculus
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
1.14.13.39
wild-type and mutants from insect cells
Mus musculus
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
1.14.13.39
macrophage
-
Mus musculus
-
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [Ámol/min/mg]
Specific Activity Maximum [Ámol/min/mg]
Commentary
Organism
1.14.13.39
additional information
-
wild-type and mutants
Mus musculus
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.14.13.39
2 L-arginine + 3 NADPH + 4 O2 + 3 H+
-
440234
Mus musculus
2 L-citrulline + 2 NO + 3 NADP+ + 4 H2O
-
440234
Mus musculus
?
1.14.13.39
oxidized cytochrome c + NADPH + O2
wild-type and mutants
440234
Mus musculus
reduced cytochrome c + NADP+ + H2O
-
-
-
?