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Literature summary extracted from
- Reactions catalyzed by tetrahydrobiopterin-free nitric oxide synthase (1998), Biochemistry, 37, 15503-15512.
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 1.14.13.39 | expression in Escherichia coli, coexpression of calmodulin is necessary | Mus musculus |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.14.13.39 | CO | - |
Mus musculus |  |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.14.13.39 | 0.036 | - |
Ngamma-hydroxy-L-arginine | 25°C, with tetrahydrobiopterin | Mus musculus | |
| 1.14.13.39 | 0.129 | - |
Ngamma-hydroxy-L-arginine | 25°C, tetrahydrobiopterin-free | Mus musculus | |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|---|
| 1.14.13.39 | additional information | 3 distinct isoforms: 1. a membrane-associated, constitutive enzyme from the vascular endothelium, 2. a soluble, constitutive enzyme from neuronal cells, 3. an endotoxin- and cytokine-inducible enzyme exemplified by that from murine macrophages | Mus musculus | - |
- |
| 1.14.13.39 | additional information | activity of constitutive enzymes is regulated by binding of calmodulin and Ca2+, the inducible enzyme is regulated by binding of calmodulin, not by Ca2+ | Mus musculus | - |
- |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.14.13.39 | Iron | protoporphyrin IX heme | Mus musculus | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.14.13.39 | Mus musculus | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 1.14.13.39 | recombinant from Escherichia coli | Mus musculus |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 1.14.13.39 | macrophage | RAW 264.7 cells | Mus musculus | - |
| 1.14.13.39 | additional information | 3 distinct isoforms: 1. a membrane-associated, constitutive enzyme from tha vascular endothelium, 2. a soluble, constitutive enzyme from neuronal cells, 3. an endotoxin- and cytokine-inducible enzyme exemplified by that from murine macrophages | Mus musculus | - |
| 1.14.13.39 | additional information | activity of constitutive enzymes is regulated by binding of calmodulin and Ca2+, the inducible enzyme is regulated by binding of calmodulin, not by Ca2+ | Mus musculus | - |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 1.14.13.39 | 0.031 | - |
purified recombinant enzyme, NADPH/O2-supported reduction of Ngamma-hydroxy-L-arginine, tetrahydrobiopterin-free, 25°C | Mus musculus |
| 1.14.13.39 | 0.12 | - |
purified recombinant enzyme, H2O2-supported reduction of Ngamma-hydroxy-L-arginine, tetrahydrobiopterin-free, 25°C | Mus musculus |
| 1.14.13.39 | 0.17 | - |
purified recombinant enzyme, NADPH/O2-supported reduction of Ngamma-hydroxy-L-arginine, with tetrahydrobiopterin, 25°C | Mus musculus |
| 1.14.13.39 | 0.815 | - |
purified recombinant enzyme, H2O2-supported reduction of Ngamma-hydroxy-L-arginine, with tetrahydrobiopterin, 25°C | Mus musculus |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.14.13.39 | 2 L-arginine + 3 NADPH + 4 O2 + 3 H+ | tetrahydrobiopterin is absolutely required for partial reaction 1 | Mus musculus | 2 L-citrulline + 2 NO + 3 NADP+ + 4 H2O | - |
? | |
| 1.14.13.39 | Ngamma-hydroxy-L-arginine + H2O2 | tetrahydrobiopterin-free | Mus musculus | citrulline + Ndelta-cyanoornithine + NO2- + NO3- | NO2-/NO3- as aerobic decomposition products from NO- | ? | |
| 1.14.13.39 | Ngamma-hydroxy-L-arginine + NADPH + O2 | substrate is intermediate between reaction 1 and 2 to form citrulline and NO from L-arginine | Mus musculus | citrulline + NADP+ + NO | - |
? | |
| 1.14.13.39 | Ngamma-hydroxy-L-arginine + NADPH + O2 | reaction is possible without tetrahydrobiopterin, can also use H2O2 instead of NADPH and O2 | Mus musculus | citrulline + NADP+ + NO | - |
? | |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 1.14.13.39 | 25 | - |
assay at | Mus musculus |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 1.14.13.39 | 37 | - |
tetrahydrobiopterin-free enzyme is not stable, therefore reaction is performed at 25°C | Mus musculus |
Cofactor
| EC Number | Cofactor | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.14.13.39 | 5,6,7,8-tetrahydro-L-biopterin | required | Mus musculus | |
| 1.14.13.39 | 5,6,7,8-tetrahydro-L-biopterin | required for the first partial reaction, formation of NG-hydroxy-L-arginine | Mus musculus | |
| 1.14.13.39 | FAD | - |
Mus musculus | |
| 1.14.13.39 | FMN | - |
Mus musculus | |
| 1.14.13.39 | NADPH | - |
Mus musculus | |
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