Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Leber, A.; Hemmens, B.; Klosch, B.; Goessler, W.; Raber, G.; Mayer, B.; Schmidt, K.
    Characterization of recombinant human endothelial nitric-oxide synthase purified from the yeast Pichia pastoris (1999), J. Biol. Chem., 274, 37658-37664.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.14.13.39 endothelial enzyme expressed in Pichia pastoris using a highly inducible alcohol oxidase promoter PAOX1 Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.14.13.39 0.0039
-
 L-arginine recombinant from Pichia pastoris Homo sapiens

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.14.13.39 Iron 0.8 mol per mol of subunit Homo sapiens
1.14.13.39 Iron heme-iron Homo sapiens
1.14.13.39 Zinc 0.43 mol per mol of subunit Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
1.14.13.39 Homo sapiens
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.14.13.39 recombinant from Pichia pastoris Homo sapiens

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.14.13.39 endothelium
-
 Homo sapiens
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.14.13.39 0.19
-
 purified recombinant enzyme Homo sapiens
1.14.13.39 0.34 0.35 purified enzyme, recombinant, determined as NADPH-oxidase activity Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.13.39 2 L-arginine + 3 NADPH + 4 O2 + 3 H+
-
 Homo sapiens 2 L-citrulline + 2 NO + 3 NADP+ + 4 H2O
-
 ?
1.14.13.39 oxidized cytochrome c + NADPH + O2
-
 Homo sapiens reduced cytochrome c + NADP+ + H2O
-
 ?

Subunits

EC Number Subunits Comment Organism
1.14.13.39 dimer 2 * 131000-133000, recombinant, SDS-PAGE Homo sapiens
1.14.13.39 More gel filtration: native protein is a dimer, especially in presence of tetrahydrobiopterin and L-arginine, but dissociates at low temperature to monomers during gel electrophoresis Homo sapiens

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.13.39 5,6,7,8-tetrahydro-L-biopterin absolute requirement, recombinant from Pichia pastoris Homo sapiens
1.14.13.39 Calmodulin dependent on, endothelial enzyme Homo sapiens
1.14.13.39 FAD 0.56 mol per mol of recombinant enzyme Homo sapiens
1.14.13.39 FMN 0.79 mol per mol of recombinant enzyme Homo sapiens
1.14.13.39 NADPH
-
 Homo sapiens