BRENDA - Enzyme Database

Ca2+/calmodulin-dependent cytochrome c reductase activity of brain nitric oxide synthase

Klatt, P.; Heinzel, B.; John, M.; Kastner, M.; Böhme, E.; Mayer, B.; J. Biol. Chem. 267, 11374-11378 (1992)

Data extracted from this reference:

Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
1.14.13.39
nitroblue tetrazolium
-
Sus scrofa
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.14.13.39
0.008
0.0084
dichlorophenolindophenol
-
Sus scrofa
1.14.13.39
0.016
-
nitroblue tetrazolium
-
Sus scrofa
1.14.13.39
0.0323
0.035
L-arginine
cytochrome c reduction
Sus scrofa
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
1.14.13.39
Ca2+
the enzyme bears Ca2+/calmodulin dependent cytochrome P-450 reductase activity which catalyzes cytochrome c reduction
Sus scrofa
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.14.13.39
additional information
Sus scrofa
NO represents the endogenous activator of soluble guanylyl cyclase
?
-
-
-
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
1.14.13.39
Sus scrofa
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
1.14.13.39
-
Sus scrofa
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
1.14.13.39
brain
-
Sus scrofa
-
1.14.13.39
cerebellum
-
Sus scrofa
-
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
1.14.13.39
0.01
-
reductase activity
Sus scrofa
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.14.13.39
2 L-arginine + 3 NADPH + 4 O2 + 3 H+
-
440199
Sus scrofa
2 L-citrulline + 2 NO + 3 NADP+ + 4 H2O
the product is a guanylyl-cyclase-relaxing factor, that is identical with nitric oxide or a NO-releasing compound
440199
Sus scrofa
?
1.14.13.39
2,6-dichlorophenolindophenol + NADPH + O2
-
440199
Sus scrofa
? + NO + NADP+
-
-
-
-
1.14.13.39
additional information
the reductase domain has a broad substrate specificity, catalyzes a moderate Ca2+/calmodulin independent hydroxylation when the enzyme is reconstituted with purified P-450
440199
Sus scrofa
?
-
-
-
-
1.14.13.39
additional information
NO represents the endogenous activator of soluble guanylyl cyclase
440199
Sus scrofa
?
-
-
-
-
1.14.13.39
nitroblue tetrazolium + NADPH
-
440199
Sus scrofa
nitroblue tetrazolium-flavazone + NADP+
-
-
-
-
1.14.13.39
oxidized cytochrome c + NADPH + O2
reaction is enhanced by addition of calmodulin at 0.0002 mM
440199
Sus scrofa
reduced cytochrome c + NADP+ + H2O
-
-
-
?
Temperature Optimum [°C]
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
1.14.13.39
37
-
assay at
Sus scrofa
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
1.14.13.39
2,6-dichlorophenolindophenol
activation
Sus scrofa
1.14.13.39
5,6,7,8-tetrahydro-L-biopterin
-
Sus scrofa
1.14.13.39
Calmodulin
the enzyme bears a Ca2+/calmodulin dependent FAD and FMN containing reductase domain which transfers electrons from NADPH to a variety of acceptors
Sus scrofa
1.14.13.39
cytochrome c
activation
Sus scrofa
1.14.13.39
FAD
the enzyme bears Ca2+/calmodulin dependent FAD and FMN containing reductase domain which transfers electrons from NADPH to a variety of acceptors
Sus scrofa
1.14.13.39
FMN
the enzyme bears Ca2+/calmodulin dependent FAD and FMN containing reductase domain which transfers electrons from NADPH to a variety of acceptors
Sus scrofa
1.14.13.39
NADPH
-
Sus scrofa
1.14.13.39
nitroblue tetrazolium
activation
Sus scrofa
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
1.14.13.39
2,6-dichlorophenolindophenol
activation
Sus scrofa
1.14.13.39
5,6,7,8-tetrahydro-L-biopterin
-
Sus scrofa
1.14.13.39
Calmodulin
the enzyme bears a Ca2+/calmodulin dependent FAD and FMN containing reductase domain which transfers electrons from NADPH to a variety of acceptors
Sus scrofa
1.14.13.39
cytochrome c
activation
Sus scrofa
1.14.13.39
FAD
the enzyme bears Ca2+/calmodulin dependent FAD and FMN containing reductase domain which transfers electrons from NADPH to a variety of acceptors
Sus scrofa
1.14.13.39
FMN
the enzyme bears Ca2+/calmodulin dependent FAD and FMN containing reductase domain which transfers electrons from NADPH to a variety of acceptors
Sus scrofa
1.14.13.39
NADPH
-
Sus scrofa
1.14.13.39
nitroblue tetrazolium
activation
Sus scrofa
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
1.14.13.39
nitroblue tetrazolium
-
Sus scrofa
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.14.13.39
0.008
0.0084
dichlorophenolindophenol
-
Sus scrofa
1.14.13.39
0.016
-
nitroblue tetrazolium
-
Sus scrofa
1.14.13.39
0.0323
0.035
L-arginine
cytochrome c reduction
Sus scrofa
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
1.14.13.39
Ca2+
the enzyme bears Ca2+/calmodulin dependent cytochrome P-450 reductase activity which catalyzes cytochrome c reduction
Sus scrofa
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.14.13.39
additional information
Sus scrofa
NO represents the endogenous activator of soluble guanylyl cyclase
?
-
-
-
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
1.14.13.39
-
Sus scrofa
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
1.14.13.39
brain
-
Sus scrofa
-
1.14.13.39
cerebellum
-
Sus scrofa
-
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
1.14.13.39
0.01
-
reductase activity
Sus scrofa
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.14.13.39
2 L-arginine + 3 NADPH + 4 O2 + 3 H+
-
440199
Sus scrofa
2 L-citrulline + 2 NO + 3 NADP+ + 4 H2O
the product is a guanylyl-cyclase-relaxing factor, that is identical with nitric oxide or a NO-releasing compound
440199
Sus scrofa
?
1.14.13.39
2,6-dichlorophenolindophenol + NADPH + O2
-
440199
Sus scrofa
? + NO + NADP+
-
-
-
-
1.14.13.39
additional information
the reductase domain has a broad substrate specificity, catalyzes a moderate Ca2+/calmodulin independent hydroxylation when the enzyme is reconstituted with purified P-450
440199
Sus scrofa
?
-
-
-
-
1.14.13.39
additional information
NO represents the endogenous activator of soluble guanylyl cyclase
440199
Sus scrofa
?
-
-
-
-
1.14.13.39
nitroblue tetrazolium + NADPH
-
440199
Sus scrofa
nitroblue tetrazolium-flavazone + NADP+
-
-
-
-
1.14.13.39
oxidized cytochrome c + NADPH + O2
reaction is enhanced by addition of calmodulin at 0.0002 mM
440199
Sus scrofa
reduced cytochrome c + NADP+ + H2O
-
-
-
?
Temperature Optimum [°C] (protein specific)
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
1.14.13.39
37
-
assay at
Sus scrofa