EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
1.18.6.1 | H195G | alpha-His of MoFe protein, site directed mutagenesis, reduced MoFe protein activity, slightly decreased Fe protein activity, altered phenotype | Azotobacter vinelandii |
1.18.6.1 | H195L | alpha-His of MoFe protein, site directed mutagenesis, reduced MoFe protein activity, increased Fe protein activity, altered phenotype | Azotobacter vinelandii |
1.18.6.1 | H195N | alpha-His of MoFe protein, site directed mutagenesis, reduced MoFe protein activity, altered phenotype | Azotobacter vinelandii |
1.18.6.1 | H195Q | alpha-His of MoFe protein, site directed mutagenesis, decreased MoFe protein activity, altered phenotype | Azotobacter vinelandii |
1.18.6.1 | H195T | alpha-His of MoFe protein, site directed mutagenesis, reduced MoFe protein and Fe protein activity, altered phenotype | Azotobacter vinelandii |
1.18.6.1 | H195Y | alpha-His of MoFe protein, site directed mutagenesis, reduced MoFe protein and Fe protein activity, altered phenotype | Azotobacter vinelandii |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
1.18.6.1 | CO | inhibition of H+ reduction by about 50% | Azotobacter vinelandii | |
1.18.6.1 | N2 | inhibits the C2H2 reduction | Azotobacter vinelandii |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.18.6.1 | additional information | - |
additional information | - |
Azotobacter vinelandii |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
1.18.6.1 | additional information | structure and organization of metal clusters | Azotobacter vinelandii |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.18.6.1 | Azotobacter vinelandii | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.18.6.1 | wild-type and mutant H195Q | Azotobacter vinelandii |
EC Number | Reaction | Comment | Organism | Reaction ID |
---|---|---|---|---|
1.18.6.1 | 4 reduced ferredoxin + 8 H+ + N2 + 16 ATP + 16 H2O = 4 oxidized ferredoxin + H2 + 2 NH3 + 16 ADP + 16 phosphate | enzyme is composed of 2 metalloproteins: component I MoFe protein and component II Fe protein | Azotobacter vinelandii |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
1.18.6.1 | additional information | - |
wild-type and diverse alpha-His195 MoFe protein mutants | Azotobacter vinelandii |
1.18.6.1 | additional information | - |
assay in anaerobic atmosphere required | Azotobacter vinelandii |
1.18.6.1 | 0.066 | - |
crude extract, 100% Ar atmosphere, H2 production | Azotobacter vinelandii |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.18.6.1 | 2 reduced ferredoxin + 2 H+ + acetylene + 2 ATP + 2 H2O | anaerobic atmosphere | Azotobacter vinelandii | 2 oxidized ferredoxin + ethylene + 2 ADP + 2 phosphate | reduction cycle continues until complete reduction of the substrate to ethane | ? | |
1.18.6.1 | 8 reduced ferredoxin + 8 H+ + N2 + 16 ATP + 16 H2O | - |
Azotobacter vinelandii | 8 oxidized ferredoxin + H2 + 2 NH3 + 16 ADP + 16 phosphate | - |
? | |
1.18.6.1 | reduced ferredoxin + H+ + ATP | - |
Azotobacter vinelandii | oxidized ferredoxin + H2 + ADP + phosphate | - |
? |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.18.6.1 | additional information | structure of MoFe-cofactor | Azotobacter vinelandii |
EC Number | Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.18.6.1 | additional information | - |
additional information | - |
Azotobacter vinelandii |