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Literature summary extracted from

  • Blanchard, C.Z.; Hales, B.J.
    Isolation of two forms of the nitrogenase VFe protein from Azotobacter vinelandii (1996), Biochemistry, 35, 472-478.
    View publication on PubMed

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.18.6.1 Iron VFe protein form 1 is an incomplete form that contains only 1 cofactor and 1 [4Fe-4S] cluster with an additional [Fe4-S4]-like cluster Azotobacter vinelandii
1.18.6.1 Iron contains [4Fe4S] cluster Azotobacter vinelandii
1.18.6.1 additional information structure and organization of metal clusters Azotobacter vinelandii
1.18.6.1 Vanadium VFe protein form 1 is an incomplete form that contains only 1 cofactor and 1 [4Fe-4S] cluster with an additional [Fe4-S4]-like cluster Azotobacter vinelandii
1.18.6.1 Vanadium 2 forms of VFe protein: form 1 has V-toFe ratio of 1:19, form 2 of 1:15 Azotobacter vinelandii

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.18.6.1 8 reduced ferredoxin + 8 H+ + N2 + 16 ATP + 16 H2O Azotobacter vinelandii biological N2 fixation 8 oxidized ferredoxin + H2 + 2 NH3 + 16 ADP + 16 phosphate
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Organism

EC Number Organism UniProt Comment Textmining
1.18.6.1 Azotobacter vinelandii
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2 forms of VFe protein
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Purification (Commentary)

EC Number Purification (Comment) Organism
1.18.6.1 2 forms of VFe protein Azotobacter vinelandii

Reaction

EC Number Reaction Comment Organism Reaction ID
1.18.6.1 4 reduced ferredoxin + 8 H+ + N2 + 16 ATP + 16 H2O = 4 oxidized ferredoxin + H2 + 2 NH3 + 16 ADP + 16 phosphate structure of V-containing enzyme form Azotobacter vinelandii

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.18.6.1 2 reduced ferredoxin + 2 H+ + acetylene + 2 ATP + 2 H2O
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Azotobacter vinelandii 2 oxidized ferredoxin + ethylene + 2 ADP + 2 phosphate
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?
1.18.6.1 8 reduced ferredoxin + 8 H+ + N2 + 16 ATP + 16 H2O
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Azotobacter vinelandii 8 oxidized ferredoxin + H2 + 2 NH3 + 16 ADP + 16 phosphate
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?
1.18.6.1 8 reduced ferredoxin + 8 H+ + N2 + 16 ATP + 16 H2O biological N2 fixation Azotobacter vinelandii 8 oxidized ferredoxin + H2 + 2 NH3 + 16 ADP + 16 phosphate
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Subunits

EC Number Subunits Comment Organism
1.18.6.1 More VFe protein form 1 has alphabeta2 conformation, VFe protein form 2 has alpha2beta2 conformation Azotobacter vinelandii