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Literature summary extracted from

  • Hallenbeck, P.C.; Kostel, P.J.; Benemann, J.R.
    Purification and properties of nitrogenase from the cyanobacterium, Anabaena cylindrica (1979), Eur. J. Biochem., 98, 275-284.
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.18.6.1 additional information
-
 additional information
-
 Anabaena cylindrica

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.18.6.1 Iron
-
 Anabaena cylindrica
1.18.6.1 Molybdenum
-
 Anabaena cylindrica

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.18.6.1 220000
-
 MoFe protein, gel filtration Anabaena cylindrica

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.18.6.1 2 reduced ferredoxin + 2 H+ + acetylene + 2 ATP + 2 H2O Anabaena cylindrica
-
 2 oxidized ferredoxin + ethylene + 2 ADP + 2 phosphate
-
 ?
1.18.6.1 8 reduced ferredoxin + 8 H+ + N2 + 16 ATP + 16 H2O Anabaena cylindrica
-
 8 oxidized ferredoxin + H2 + 2 NH3 + 16 ADP + 16 phosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.18.6.1 Anabaena cylindrica
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.18.6.1
-
 Anabaena cylindrica

Reaction

EC Number Reaction Comment Organism Reaction ID
1.18.6.1 4 reduced ferredoxin + 8 H+ + N2 + 16 ATP + 16 H2O = 4 oxidized ferredoxin + H2 + 2 NH3 + 16 ADP + 16 phosphate enzyme is composed of 2 metalloproteins: component I MoFe protein and component II Fe protein Anabaena cylindrica
a

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.18.6.1 additional information
-
-
 Anabaena cylindrica

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.18.6.1 2 reduced ferredoxin + 2 H+ + acetylene + 2 ATP + 2 H2O
-
 Anabaena cylindrica 2 oxidized ferredoxin + ethylene + 2 ADP + 2 phosphate
-
 ?
1.18.6.1 8 reduced ferredoxin + 8 H+ + N2 + 16 ATP + 16 H2O
-
 Anabaena cylindrica 8 oxidized ferredoxin + H2 + 2 NH3 + 16 ADP + 16 phosphate
-
 ?
1.18.6.1 dithionite + H+ + N2 + ATP in vitro substrate Anabaena cylindrica ?
-
 ?

Subunits

EC Number Subunits Comment Organism
1.18.6.1 More
-
 Anabaena cylindrica