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Literature summary extracted from

  • Yates, M.G.; Planque, K.
    Nitrogenase from Azotobacter chroococcum. Purification and properties of the component proteins (1975), Eur. J. Biochem., 60, 467-476.
    View publication on PubMed

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.18.6.1 Iron 4 atoms of iron per molecule of Fe protein Azotobacter chroococcum
1.18.6.1 Molybdenum 23 mol Fe + 1.9 mol Mo per mol of MoFe protein Azotobacter chroococcum

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.18.6.1 additional information
-
-
 Azotobacter chroococcum
1.18.6.1 30800
-
 MoFe protein 4 * 60000 + Fe protein 2 * 30800, SDS-PAGE Azotobacter chroococcum
1.18.6.1 60000
-
 MoFe protein 4 * 60000 + Fe protein 2 * 30800, SDS-PAGE Azotobacter chroococcum
1.18.6.1 64000
-
 Fe protein, gel filtration Azotobacter chroococcum
1.18.6.1 227000
-
 MoFe protein, gel filtration Azotobacter chroococcum

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.18.6.1 8 reduced ferredoxin + 8 H+ + N2 + 16 ATP + 16 H2O Azotobacter chroococcum
-
 8 oxidized ferredoxin + H2 + 2 NH3 + 16 ADP + 16 phosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.18.6.1 Azotobacter chroococcum
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.18.6.1 large scale Azotobacter chroococcum

Reaction

EC Number Reaction Comment Organism Reaction ID
1.18.6.1 4 reduced ferredoxin + 8 H+ + N2 + 16 ATP + 16 H2O = 4 oxidized ferredoxin + H2 + 2 NH3 + 16 ADP + 16 phosphate enzyme is composed of 2 metalloproteins: component I MoFe protein and component II Fe protein Azotobacter chroococcum
a

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.18.6.1 8 reduced ferredoxin + 8 H+ + N2 + 16 ATP + 16 H2O
-
 Azotobacter chroococcum 8 oxidized ferredoxin + H2 + 2 NH3 + 16 ADP + 16 phosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
1.18.6.1 ? MoFe protein 4 * 60000 + Fe protein 2 * 30800, SDS-PAGE Azotobacter chroococcum

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
1.18.6.1 5 8 MoFe protein stable Azotobacter chroococcum
1.18.6.1 8.7
-
 50% loss of activity after overnight dialysis at pH 8.7 Azotobacter chroococcum