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Literature summary extracted from

  • Fuhrmann, M.; Hennecke, H.
    Coding properties of cloned nitrogenase structural genes from Rhizobium japonicum (1982), Mol. Gen. Genet., 187, 419-425.
No PubMed abstract available

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.18.6.1 expression in Escherichia coli Bradyrhizobium japonicum

Protein Variants

EC Number Protein Variants Comment Organism
1.18.6.1 additional information transposon insertion mutants of several plasmids Bradyrhizobium japonicum

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.18.6.1 8 reduced ferredoxin + 8 H+ + N2 + 16 ATP + 16 H2O Bradyrhizobium japonicum
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8 oxidized ferredoxin + H2 + 2 NH3 + 16 ADP + 16 phosphate
-
?

Organism

EC Number Organism UniProt Comment Textmining
1.18.6.1 Bradyrhizobium japonicum
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nitrogen fixation complex is encoded on nif gene cluster
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Reaction

EC Number Reaction Comment Organism Reaction ID
1.18.6.1 4 reduced ferredoxin + 8 H+ + N2 + 16 ATP + 16 H2O = 4 oxidized ferredoxin + H2 + 2 NH3 + 16 ADP + 16 phosphate enzyme is composed of 2 metalloproteins: component I MoFe protein and component II Fe protein Bradyrhizobium japonicum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.18.6.1 8 reduced ferredoxin + 8 H+ + N2 + 16 ATP + 16 H2O
-
Bradyrhizobium japonicum 8 oxidized ferredoxin + H2 + 2 NH3 + 16 ADP + 16 phosphate
-
?