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Literature summary extracted from

  • Shah, V.K.; Ugalde, R.A.; Imperial, J.; Brill, W.J.
    Molybdenum in nitrogenase (1984), Annu. Rev. Biochem., 53, 231-257.
    View publication on PubMed

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
1.18.6.1
-
 Azotobacter vinelandii

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.18.6.1 CO
-
 Azotobacter vinelandii
1.18.6.1 CO
-
 Clostridium pasteurianum
1.18.6.1 CO
-
 Klebsiella pneumoniae
1.18.6.1 CO
-
 Paenibacillus polymyxa
1.18.6.1 O2
-
 Azotobacter vinelandii
1.18.6.1 O2
-
 Clostridium pasteurianum
1.18.6.1 O2
-
 Klebsiella pneumoniae
1.18.6.1 O2
-
 Paenibacillus polymyxa

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.18.6.1 Iron
-
 Clostridium pasteurianum
1.18.6.1 Iron
-
 Paenibacillus polymyxa
1.18.6.1 Iron enzyme consists of 2 proteins: a molybdenum and iron-containing protein, MoFe protein, component I, dinitrogenase, and an iron containing protein, Fe protein, component II, dinitrogenase reductase, together they form the active nitrogenase complex Klebsiella pneumoniae
1.18.6.1 Iron enzyme consists of 2 proteins: a molybdenum and iron-containing protein, MoFe protein, component I, dinitrogenase, and an iron containing protein, Fe protein, component II, dinitrogenase reductase, together they form the active nitrogenase complex Azotobacter vinelandii
1.18.6.1 Iron 4 atoms of iron per molecule of Fe protein Klebsiella pneumoniae
1.18.6.1 Iron 4 atoms of iron per molecule of Fe protein Azotobacter vinelandii
1.18.6.1 Mg2+ Mg2+ required for MgATP complex Klebsiella pneumoniae
1.18.6.1 Mg2+ Mg2+ required for MgATP complex Clostridium pasteurianum
1.18.6.1 Mg2+ Mg2+ required for MgATP complex Azotobacter vinelandii
1.18.6.1 Mg2+ Mg2+ required for MgATP complex Paenibacillus polymyxa
1.18.6.1 Molybdenum review on molybdenum in nitrogenase Klebsiella pneumoniae
1.18.6.1 Molybdenum review on molybdenum in nitrogenase Clostridium pasteurianum
1.18.6.1 Molybdenum review on molybdenum in nitrogenase Azotobacter vinelandii
1.18.6.1 Molybdenum review on molybdenum in nitrogenase Paenibacillus polymyxa
1.18.6.1 Molybdenum 6 iron atoms to 1 molybdenum atom in MoFe protein Klebsiella pneumoniae
1.18.6.1 Molybdenum molybdenum metabolism, cofactor synthesis from nif genes , regulation and structure, overview Klebsiella pneumoniae
1.18.6.1 Molybdenum molybdenum metabolism, cofactor synthesis from nif genes , regulation and structure, overview Clostridium pasteurianum
1.18.6.1 Molybdenum molybdenum metabolism, cofactor synthesis from nif genes , regulation and structure, overview Azotobacter vinelandii
1.18.6.1 Molybdenum enzyme consists of 2 proteins: a molybdenum and iron-containing protein (MoFe protein, component I, dinitrogenase) and an iron containing protein (Fe protein, component II, dinitrogenase reductase), together they form the active nitrogenase complex Klebsiella pneumoniae
1.18.6.1 Molybdenum enzyme consists of 2 proteins: a molybdenum and iron-containing protein (MoFe protein, component I, dinitrogenase) and an iron containing protein (Fe protein, component II, dinitrogenase reductase), together they form the active nitrogenase complex Azotobacter vinelandii

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.18.6.1 55000 60000 component II Fe protein Klebsiella pneumoniae
1.18.6.1 55000 60000 component II Fe protein Clostridium pasteurianum
1.18.6.1 55000 60000 component II Fe protein Azotobacter vinelandii
1.18.6.1 220000 250000 component I MoFe protein Klebsiella pneumoniae
1.18.6.1 220000 250000 component I MoFe protein Clostridium pasteurianum
1.18.6.1 220000 250000 component I MoFe protein Azotobacter vinelandii

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.18.6.1 8 reduced ferredoxin + 8 H+ + N2 + 16 ATP + 16 H2O Klebsiella pneumoniae
-
 8 oxidized ferredoxin + H2 + 2 NH3 + 16 ADP + 16 phosphate
-
 ?
1.18.6.1 8 reduced ferredoxin + 8 H+ + N2 + 16 ATP + 16 H2O Clostridium pasteurianum
-
 8 oxidized ferredoxin + H2 + 2 NH3 + 16 ADP + 16 phosphate
-
 ?
1.18.6.1 8 reduced ferredoxin + 8 H+ + N2 + 16 ATP + 16 H2O Azotobacter vinelandii
-
 8 oxidized ferredoxin + H2 + 2 NH3 + 16 ADP + 16 phosphate
-
 ?
1.18.6.1 8 reduced ferredoxin + 8 H+ + N2 + 16 ATP + 16 H2O Paenibacillus polymyxa
-
 8 oxidized ferredoxin + H2 + 2 NH3 + 16 ADP + 16 phosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.18.6.1 Azotobacter vinelandii
-
-
-
1.18.6.1 Clostridium pasteurianum
-
-
-
1.18.6.1 Klebsiella pneumoniae
-
 nitrogen fixation complex is encoded on nif gene cluster
-
1.18.6.1 Paenibacillus polymyxa
-
-
-

Oxidation Stability

EC Number Oxidation Stability Organism
1.18.6.1 MoFe protein is extremely sensitive to O2 Klebsiella pneumoniae
1.18.6.1 MoFe protein is extremely sensitive to O2 Azotobacter vinelandii

Reaction

EC Number Reaction Comment Organism Reaction ID
1.18.6.1 4 reduced ferredoxin + 8 H+ + N2 + 16 ATP + 16 H2O = 4 oxidized ferredoxin + H2 + 2 NH3 + 16 ADP + 16 phosphate catalytic mechanism Klebsiella pneumoniae
a
1.18.6.1 4 reduced ferredoxin + 8 H+ + N2 + 16 ATP + 16 H2O = 4 oxidized ferredoxin + H2 + 2 NH3 + 16 ADP + 16 phosphate catalytic mechanism Clostridium pasteurianum
a
1.18.6.1 4 reduced ferredoxin + 8 H+ + N2 + 16 ATP + 16 H2O = 4 oxidized ferredoxin + H2 + 2 NH3 + 16 ADP + 16 phosphate catalytic mechanism Azotobacter vinelandii
a
1.18.6.1 4 reduced ferredoxin + 8 H+ + N2 + 16 ATP + 16 H2O = 4 oxidized ferredoxin + H2 + 2 NH3 + 16 ADP + 16 phosphate catalytic mechanism Paenibacillus polymyxa
a
1.18.6.1 4 reduced ferredoxin + 8 H+ + N2 + 16 ATP + 16 H2O = 4 oxidized ferredoxin + H2 + 2 NH3 + 16 ADP + 16 phosphate enzyme is composed of 2 metalloproteins: component I MoFe protein and component II Fe protein Klebsiella pneumoniae
a
1.18.6.1 4 reduced ferredoxin + 8 H+ + N2 + 16 ATP + 16 H2O = 4 oxidized ferredoxin + H2 + 2 NH3 + 16 ADP + 16 phosphate enzyme is composed of 2 metalloproteins: component I MoFe protein and component II Fe protein Clostridium pasteurianum
a
1.18.6.1 4 reduced ferredoxin + 8 H+ + N2 + 16 ATP + 16 H2O = 4 oxidized ferredoxin + H2 + 2 NH3 + 16 ADP + 16 phosphate enzyme is composed of 2 metalloproteins: component I MoFe protein and component II Fe protein Azotobacter vinelandii
a
1.18.6.1 4 reduced ferredoxin + 8 H+ + N2 + 16 ATP + 16 H2O = 4 oxidized ferredoxin + H2 + 2 NH3 + 16 ADP + 16 phosphate enzyme is composed of 2 metalloproteins: component I MoFe protein and component II Fe protein Paenibacillus polymyxa
a

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.18.6.1 8 reduced ferredoxin + 8 H+ + N2 + 16 ATP + 16 H2O
-
 Klebsiella pneumoniae 8 oxidized ferredoxin + H2 + 2 NH3 + 16 ADP + 16 phosphate
-
 ?
1.18.6.1 8 reduced ferredoxin + 8 H+ + N2 + 16 ATP + 16 H2O
-
 Clostridium pasteurianum 8 oxidized ferredoxin + H2 + 2 NH3 + 16 ADP + 16 phosphate
-
 ?
1.18.6.1 8 reduced ferredoxin + 8 H+ + N2 + 16 ATP + 16 H2O
-
 Azotobacter vinelandii 8 oxidized ferredoxin + H2 + 2 NH3 + 16 ADP + 16 phosphate
-
 ?
1.18.6.1 8 reduced ferredoxin + 8 H+ + N2 + 16 ATP + 16 H2O
-
 Paenibacillus polymyxa 8 oxidized ferredoxin + H2 + 2 NH3 + 16 ADP + 16 phosphate
-
 ?