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Literature summary extracted from

  • Hagenfeldt, Y.; Pedersen, J.I.; Björkhem, I.
    Properties of guinea-pig kidney 25-hydroxyvitamin D3 1 alpha-hydroxylase assayed by isotope dilution-mass spectrometry (1988), Biochem. J., 250, 521-526.
    View publication on PubMedView publication on EuropePMC

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.14.15.18 1,25-Dihydroxycholecalciferol calcitriol Cavia porcellus
1.14.15.18 antimycin A no inhibition of hydroxylation Cavia porcellus
1.14.15.18 CO
-
 Cavia porcellus
1.14.15.18 cyanide
-
 Cavia porcellus
1.14.15.18 p-Trifluoromethoxyphenylhydrazone
-
 Cavia porcellus
1.14.15.18 rotenone no inhibition of hydroxylation Cavia porcellus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.14.15.18 0.001
-
 25-hydroxycholecalciferol
-
 Cavia porcellus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.14.15.18 mitochondrion
-
 Cavia porcellus 5739
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.14.15.18 Mg2+ 10 mM used in standard incubation Cavia porcellus
1.14.15.18 additional information highest conversion with a hypo-osmolar buffer Cavia porcellus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.14.15.18 25-hydroxycholecalciferol + NADPH + O2 Cavia porcellus energy-dependent transhydrogenation is of importance calcitriol + NADP+ + H2O
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.14.15.18 Cavia porcellus
-
-
-

Reaction

EC Number Reaction Comment Organism Reaction ID
1.14.15.18 calcidiol + 2 reduced adrenodoxin + 2 H+ + O2 = calcitriol + 2 oxidized adrenodoxin + H2O 25-hydroxyvitamin D3 1alpha-hydroxylase is a cytochrome P-450-dependent mixed-function oxidase Cavia porcellus
a

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.14.15.18 kidney
-
 Cavia porcellus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.15.18 25-hydroxycholecalciferol + NADPH + O2 energy-dependent transhydrogenation is of importance Cavia porcellus calcitriol + NADP+ + H2O
-
 ?
1.14.15.18 calcidiol + NADPH + O2 highly specific for calcidiol, calcidiol is identical with 25-hydroxycholecalciferol Cavia porcellus calcitriol + NADP+ + H2O calcitriol is identical with 1,25-dihydroxycholecalciferol ?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.14.15.18 7.4 8.4
-
 Cavia porcellus

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.15.18 NADPH
-
 Cavia porcellus