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Literature summary extracted from

  • Tumbula, D.L.; Becker, H.D.; Chang, W.Z.; Söll, D.
    Domain-specific recruitment of amide amino acids for protein synthesis (2000), Nature, 407, 106-110.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
6.3.5.6 cloning of the gatCAB genes encoding the Asp/Glu-AdT and overexpression in Escherichia coli Methanothermobacter thermautotrophicus
6.3.5.6 presence of the gatCAB genes encoding Asp/Glu-AdT Bacillus subtilis
6.3.5.6 presence of the gatCAB genes encoding Asp/Glu-AdT Methanothermobacter thermautotrophicus
6.3.5.6 presence of the gatCAB genes encoding Asp/Glu-AdT Halobacterium salinarum
6.3.5.6 presence of the gatCAB genes encoding Asp/Glu-AdT Saccharolobus solfataricus
6.3.5.6 presence of the gatCAB genes encoding Asp/Glu-AdT Methanocaldococcus jannaschii
6.3.5.6 presence of the gatCAB genes encoding Asp/Glu-AdT Archaeoglobus fulgidus
6.3.5.6 presence of the gatCAB genes encoding Asp/Glu-AdT Deinococcus radiodurans
6.3.5.6 presence of the gatCAB genes encoding Asp/Glu-AdT Methanosarcina mazei
6.3.5.6 presence of the gatCAB genes encoding Asp/Glu-AdT Aeropyrum pernix
6.3.5.6 presence of the gatCAB genes encoding Asp/Glu-AdT Sulfurisphaera tokodaii

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
6.3.5.6 Asp-tRNAAsn + ? Methanothermobacter thermautotrophicus
-
Asn-tRNAAsn + ?
-
?
6.3.5.6 Asp-tRNAAsn + ? Deinococcus radiodurans tRNA-dependent transamidation pathway of Asn-tRNA formation, only biosynthetic route to asparagine is via Asn-tRNA Asn-tRNAAsn + ?
-
?
6.3.5.6 Asp-tRNAAsn + ? Chlamydia trachomatis it is likely that the dual specificity amidotransferase serves in Asn-tRNA and Gln-tRNA formation in vivo Asn-tRNAAsn + ?
-
?
6.3.5.6 Asp-tRNAAsn + ? Helicobacter pylori it is likely that the dual specificity amidotransferase serves in Asn-tRNA and Gln-tRNA formation in vivo Asn-tRNAAsn + ?
-
?
6.3.5.6 Asp-tRNAAsn + ? Deinococcus radiodurans in vivo only Asn-tRNAAsn formation occurs Asn-tRNAAsn + ?
-
?
6.3.5.6 Asp-tRNAAsn + ? Methanothermobacter thermautotrophicus DELTAH
-
Asn-tRNAAsn + ?
-
?
6.3.5.6 Glu-tRNAGln + ? Chlamydia trachomatis it is likely that the dual specificity amidotransferase serves in Asn-tRNA and Gln-tRNA formation in vivo Gln-tRNAGln + ?
-
?
6.3.5.6 Glu-tRNAGln + ? Helicobacter pylori it is likely that the dual specificity amidotransferase serves in Asn-tRNA and Gln-tRNA formation in vivo Gln-tRNAGln + ?
-
?

Organism

EC Number Organism UniProt Comment Textmining
6.3.5.6 Aeropyrum pernix
-
-
-
6.3.5.6 Archaeoglobus fulgidus
-
-
-
6.3.5.6 Bacillus subtilis
-
-
-
6.3.5.6 Chlamydia trachomatis
-
-
-
6.3.5.6 Deinococcus radiodurans
-
-
-
6.3.5.6 Halobacterium salinarum
-
-
-
6.3.5.6 Helicobacter pylori
-
-
-
6.3.5.6 Methanocaldococcus jannaschii
-
-
-
6.3.5.6 Methanosarcina mazei
-
-
-
6.3.5.6 Methanothermobacter thermautotrophicus
-
-
-
6.3.5.6 Methanothermobacter thermautotrophicus
-
Methanobacterium thermoautotrophicum
-
6.3.5.6 Methanothermobacter thermautotrophicus DELTAH
-
-
-
6.3.5.6 Saccharolobus solfataricus
-
-
-
6.3.5.6 Sulfurisphaera tokodaii
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
6.3.5.6 purification of recombinant enzyme, expressed in Escherichia coli Methanothermobacter thermautotrophicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.3.5.6 Asp-tRNAAsn + ?
-
Methanothermobacter thermautotrophicus Asn-tRNAAsn + ?
-
?
6.3.5.6 Asp-tRNAAsn + ? tRNA-dependent transamidation pathway of Asn-tRNA formation, only biosynthetic route to asparagine is via Asn-tRNA Deinococcus radiodurans Asn-tRNAAsn + ?
-
?
6.3.5.6 Asp-tRNAAsn + ? it is likely that the dual specificity amidotransferase serves in Asn-tRNA and Gln-tRNA formation in vivo Chlamydia trachomatis Asn-tRNAAsn + ?
-
?
6.3.5.6 Asp-tRNAAsn + ? it is likely that the dual specificity amidotransferase serves in Asn-tRNA and Gln-tRNA formation in vivo Helicobacter pylori Asn-tRNAAsn + ?
-
?
6.3.5.6 Asp-tRNAAsn + ? in vivo only Asn-tRNAAsn formation occurs Deinococcus radiodurans Asn-tRNAAsn + ?
-
?
6.3.5.6 Asp-tRNAAsn + ?
-
Methanothermobacter thermautotrophicus DELTAH Asn-tRNAAsn + ?
-
?
6.3.5.6 ATP + Asp-tRNAAsn + glutamine
-
Bacillus subtilis ADP + phosphate + Asn-tRNAAsn + glutamate
-
?
6.3.5.6 ATP + Asp-tRNAAsn + glutamine
-
Halobacterium salinarum ADP + phosphate + Asn-tRNAAsn + glutamate
-
?
6.3.5.6 ATP + Asp-tRNAAsn + glutamine
-
Saccharolobus solfataricus ADP + phosphate + Asn-tRNAAsn + glutamate
-
?
6.3.5.6 ATP + Asp-tRNAAsn + glutamine
-
Methanocaldococcus jannaschii ADP + phosphate + Asn-tRNAAsn + glutamate
-
?
6.3.5.6 ATP + Asp-tRNAAsn + glutamine
-
Archaeoglobus fulgidus ADP + phosphate + Asn-tRNAAsn + glutamate
-
?
6.3.5.6 ATP + Asp-tRNAAsn + glutamine
-
Methanosarcina mazei ADP + phosphate + Asn-tRNAAsn + glutamate
-
?
6.3.5.6 ATP + Asp-tRNAAsn + glutamine
-
Aeropyrum pernix ADP + phosphate + Asn-tRNAAsn + glutamate
-
?
6.3.5.6 ATP + Asp-tRNAAsn + glutamine
-
Sulfurisphaera tokodaii ADP + phosphate + Asn-tRNAAsn + glutamate
-
?
6.3.5.6 ATP + Asp-tRNAAsn + glutamine dual-specific Asp/Glu-AdT is able to amidate both mischarged tRNAGln and tRNAAsn, but functions as Asp-AdT Deinococcus radiodurans ADP + phosphate + Asn-tRNAAsn + glutamate
-
?
6.3.5.6 ATP + Asp-tRNAAsn + glutamine dual tRNA specificity: formation of Asn-tRNA and Gln-tRNA Chlamydia trachomatis ADP + phosphate + Asn-tRNAAsn + glutamate
-
?
6.3.5.6 ATP + Asp-tRNAAsn + glutamine dual tRNA specificity: formation of Asn-tRNA and Gln-tRNA Methanothermobacter thermautotrophicus ADP + phosphate + Asn-tRNAAsn + glutamate
-
?
6.3.5.6 ATP + Asp-tRNAAsn + glutamine dual tRNA specificity: formation of Asn-tRNA and Gln-tRNA Helicobacter pylori ADP + phosphate + Asn-tRNAAsn + glutamate
-
?
6.3.5.6 ATP + Asp-tRNAAsn + glutamine dual tRNA specificity: formation of Asn-tRNA and Gln-tRNA Deinococcus radiodurans ADP + phosphate + Asn-tRNAAsn + glutamate
-
?
6.3.5.6 ATP + Asp-tRNAAsn + glutamine dual tRNA specificity: formation of Asn-tRNA and Gln-tRNA Methanothermobacter thermautotrophicus DELTAH ADP + phosphate + Asn-tRNAAsn + glutamate
-
?
6.3.5.6 ATP + Glu-tRNAGln + glutamine
-
Deinococcus radiodurans ADP + Gln-tRNAGln + glutamate + phosphate
-
?
6.3.5.6 ATP + Glu-tRNAGln + glutamine dual tRNA specificity: formation of Asn-tRNA and Gln-tRNA Chlamydia trachomatis ADP + Gln-tRNAGln + glutamate + phosphate
-
?
6.3.5.6 ATP + Glu-tRNAGln + glutamine dual tRNA specificity: formation of Asn-tRNA and Gln-tRNA Methanothermobacter thermautotrophicus ADP + Gln-tRNAGln + glutamate + phosphate
-
?
6.3.5.6 ATP + Glu-tRNAGln + glutamine dual tRNA specificity: formation of Asn-tRNA and Gln-tRNA Helicobacter pylori ADP + Gln-tRNAGln + glutamate + phosphate
-
?
6.3.5.6 ATP + Glu-tRNAGln + glutamine Asp/Glu-AdT carries out Gln-tRNA formation, since Bacillus contains a non-discriminating GluRS, but lacks a mischarging AspRS Bacillus subtilis ADP + Gln-tRNAGln + glutamate + phosphate
-
?
6.3.5.6 ATP + Glu-tRNAGln + glutamine dual tRNA specificity: formation of Asn-tRNA and Gln-tRNA Methanothermobacter thermautotrophicus DELTAH ADP + Gln-tRNAGln + glutamate + phosphate
-
?
6.3.5.6 Glu-tRNAGln + ? it is likely that the dual specificity amidotransferase serves in Asn-tRNA and Gln-tRNA formation in vivo Chlamydia trachomatis Gln-tRNAGln + ?
-
?
6.3.5.6 Glu-tRNAGln + ? it is likely that the dual specificity amidotransferase serves in Asn-tRNA and Gln-tRNA formation in vivo Helicobacter pylori Gln-tRNAGln + ?
-
?
6.3.5.6 additional information
-
Methanocaldococcus jannaschii ?
-
?
6.3.5.6 additional information
-
Archaeoglobus fulgidus ?
-
?
6.3.5.6 additional information
-
Aeropyrum pernix ?
-
?
6.3.5.6 additional information no substrates: correctly charged Asp-tRNAAsp and Glu-tRNAGlu Chlamydia trachomatis ?
-
?
6.3.5.6 additional information no substrates: correctly charged Asp-tRNAAsp and Glu-tRNAGlu Methanothermobacter thermautotrophicus ?
-
?
6.3.5.6 additional information no substrates: correctly charged Asp-tRNAAsp and Glu-tRNAGlu Deinococcus radiodurans ?
-
?
6.3.5.6 additional information no substrates: correctly charged Asp-tRNAAsp and Glu-tRNAGlu Methanothermobacter thermautotrophicus DELTAH ?
-
?

Subunits

EC Number Subunits Comment Organism
6.3.5.6 heterotrimer
-
Chlamydia trachomatis
6.3.5.6 heterotrimer
-
Methanocaldococcus jannaschii
6.3.5.6 heterotrimer
-
Archaeoglobus fulgidus
6.3.5.6 heterotrimer
-
Aeropyrum pernix
6.3.5.6 heterotrimer GatCAB enzyme Bacillus subtilis
6.3.5.6 heterotrimer GatCAB enzyme Methanothermobacter thermautotrophicus
6.3.5.6 heterotrimer GatCAB enzyme Deinococcus radiodurans

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
6.3.5.6 37
-
assay at Chlamydia trachomatis
6.3.5.6 37
-
assay at Methanothermobacter thermautotrophicus