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Literature summary extracted from

  • Min, B.; Pelaschier, J.T.; Graham, D.E.; Tumbula-Hansen, D.; Söll, D.
    Transfer RNA-dependent amino acid biosynthesis: An essential route to asparagine formation (2002), Proc. Natl. Acad. Sci. USA, 99, 2678-2683.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
6.3.5.6 gatCAB genes encode Asp-AdT Deinococcus radiodurans

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
6.3.5.6 Asp-tRNAAsn + ? Thermus thermophilus tRNA-dependent transamidation pathway of Asn-tRNA formation, only biosynthetic route to asparagine is via Asn-tRNA Asn-tRNAAsn + ?
-
 ?
6.3.5.6 Asp-tRNAAsn + ? Deinococcus radiodurans tRNA-dependent transamidation pathway of Asn-tRNA formation, only biosynthetic route to asparagine is via Asn-tRNA Asn-tRNAAsn + ?
-
 ?
6.3.5.6 Asp-tRNAAsn + ? Thermus thermophilus enzyme of the two-step pathway for the synthesis of Asn-tRNA that requires misacylated Asp-tRNAAsn as intermediate, which is generated by a Asp-tRNA synthetase Asn-tRNAAsn + ?
-
 ?
6.3.5.6 Asp-tRNAAsn + ? Deinococcus radiodurans enzyme of the two-step pathway for the synthesis of Asn-tRNA that requires misacylated Asp-tRNAAsn as intermediate, which is generated by a Asp-tRNA synthetase Asn-tRNAAsn + ?
-
 ?
6.3.5.6 Asp-tRNAAsn + ? Deinococcus radiodurans R1 / ATCC 13939 / DSM 20539 tRNA-dependent transamidation pathway of Asn-tRNA formation, only biosynthetic route to asparagine is via Asn-tRNA Asn-tRNAAsn + ?
-
 ?
6.3.5.6 Asp-tRNAAsn + ? Deinococcus radiodurans R1 / ATCC 13939 / DSM 20539 enzyme of the two-step pathway for the synthesis of Asn-tRNA that requires misacylated Asp-tRNAAsn as intermediate, which is generated by a Asp-tRNA synthetase Asn-tRNAAsn + ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
6.3.5.6 Deinococcus radiodurans
-
-
-
6.3.5.6 Deinococcus radiodurans R1 / ATCC 13939 / DSM 20539
-
-
-
6.3.5.6 Thermus thermophilus
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.3.5.6 Asp-tRNAAsn + ? tRNA-dependent transamidation pathway of Asn-tRNA formation, only biosynthetic route to asparagine is via Asn-tRNA Thermus thermophilus Asn-tRNAAsn + ?
-
 ?
6.3.5.6 Asp-tRNAAsn + ? tRNA-dependent transamidation pathway of Asn-tRNA formation, only biosynthetic route to asparagine is via Asn-tRNA Deinococcus radiodurans Asn-tRNAAsn + ?
-
 ?
6.3.5.6 Asp-tRNAAsn + ? enzyme of the two-step pathway for the synthesis of Asn-tRNA that requires misacylated Asp-tRNAAsn as intermediate, which is generated by a Asp-tRNA synthetase Thermus thermophilus Asn-tRNAAsn + ?
-
 ?
6.3.5.6 Asp-tRNAAsn + ? enzyme of the two-step pathway for the synthesis of Asn-tRNA that requires misacylated Asp-tRNAAsn as intermediate, which is generated by a Asp-tRNA synthetase Deinococcus radiodurans Asn-tRNAAsn + ?
-
 ?
6.3.5.6 Asp-tRNAAsn + ? tRNA-dependent transamidation pathway of Asn-tRNA formation, only biosynthetic route to asparagine is via Asn-tRNA Deinococcus radiodurans R1 / ATCC 13939 / DSM 20539 Asn-tRNAAsn + ?
-
 ?
6.3.5.6 Asp-tRNAAsn + ? enzyme of the two-step pathway for the synthesis of Asn-tRNA that requires misacylated Asp-tRNAAsn as intermediate, which is generated by a Asp-tRNA synthetase Deinococcus radiodurans R1 / ATCC 13939 / DSM 20539 Asn-tRNAAsn + ?
-
 ?
6.3.5.6 ATP + Asp-tRNAAsn + glutamine
-
 Thermus thermophilus ADP + phosphate + Asn-tRNAAsn + glutamate
-
 ?
6.3.5.6 ATP + Asp-tRNAAsn + glutamine
-
 Deinococcus radiodurans ADP + phosphate + Asn-tRNAAsn + glutamate
-
 ?
6.3.5.6 ATP + Asp-tRNAAsn + glutamine
-
 Deinococcus radiodurans R1 / ATCC 13939 / DSM 20539 ADP + phosphate + Asn-tRNAAsn + glutamate
-
 ?

Subunits

EC Number Subunits Comment Organism
6.3.5.6 heterotrimer
-
 Thermus thermophilus
6.3.5.6 heterotrimer GatCAB enzyme Deinococcus radiodurans