EC Number | Cloned (Comment) | Organism |
---|---|---|
6.1.1.12 | expression as His-tagged protein in Escherichia coli | Methanothermobacter thermautotrophicus |
6.1.1.12 | expression as His-tagged protein in Escherichia coli | Thermococcus kodakarensis |
6.1.1.12 | expression as His-tagged protein in Escherichia coli | Ferroplasma acidarmanus |
6.1.1.12 | expression in Escherichia coli | Thermococcus kodakarensis |
6.1.1.12 | expression in Escherichia coli | Ferroplasma acidarmanus |
6.1.1.23 | expression in Escherichia coli | Methanothermobacter thermautotrophicus |
6.3.5.6 | cloning of the gatCAB operon encoding the Asp-tRNAAsn amidotransferase and expression in Escherichia coli BL21-Codon Plus(DE3)-RIL | Methanothermobacter thermautotrophicus |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
6.1.1.12 | ATP + L-aspartate + tRNAAsn | Methanothermobacter thermautotrophicus | the archaeal AspRS enzyme is nondiscriminating, which means that it forms Asp-tRNAAsp and Asp-tRNAAsn, which is the intermediate in AsntRNAAsn generation by ASp-tRNAAsn amidotransferase, in contrary bacterial enzymes are discriminating ones | AMP + diphosphate + L-aspartyl-tRNAAsn | - |
? | |
6.1.1.12 | ATP + L-aspartate + tRNAAsp | Methanothermobacter thermautotrophicus | - |
AMP + diphosphate + L-aspartyl-tRNAAsp | - |
? | |
6.1.1.12 | ATP + L-aspartate + tRNAAsp | Thermococcus kodakarensis | - |
AMP + diphosphate + L-aspartyl-tRNAAsp | - |
? | |
6.1.1.12 | ATP + L-aspartate + tRNAAsp | Ferroplasma acidarmanus | - |
AMP + diphosphate + L-aspartyl-tRNAAsp | - |
? | |
6.1.1.12 | additional information | Thermococcus kodakarensis | no acylation with L-aspartate of tRNAAsn, enzyme is discriminating, because it forms Asn-tRNAAsn by direct aminoacylation, not via the intermediate of Asp-tRNAAsn, and it contains no Asp-tRNAAsn amidotransferase | ? | - |
? | |
6.1.1.12 | additional information | Ferroplasma acidarmanus | no acylation with L-aspartate of tRNAAsn, enzyme is discriminating, because it forms Asn-tRNAAsn by direct aminoacylation, not via the intermediate of Asp-tRNAAsn, and it contains no Asp-tRNAAsn amidotransferase | ? | - |
? | |
6.1.1.23 | ATP + L-aspartate + tRNAAsn | Methanothermobacter thermautotrophicus | - |
AMP + diphosphate + L-aspartyl-tRNAAsn | - |
? | |
6.1.1.23 | ATP + L-aspartate + tRNAAsn | Methanothermobacter thermautotrophicus DSM 1053 | - |
AMP + diphosphate + L-aspartyl-tRNAAsn | - |
? | |
6.1.1.23 | ATP + L-aspartate + tRNAAsp | Methanothermobacter thermautotrophicus | - |
AMP + diphosphate + L-aspartyl-tRNAAsp | - |
? | |
6.1.1.23 | ATP + L-aspartate + tRNAAsp | Methanothermobacter thermautotrophicus DSM 1053 | - |
AMP + diphosphate + L-aspartyl-tRNAAsp | - |
? | |
6.3.5.6 | Asp-tRNAAsn + ? | Methanothermobacter thermautotrophicus | the mischarging Asp-tRNA synthetase correlates with the absence in the genome of Asn-tRNA synthetase and the presence of Asp-tRNAAsn amidotransferase, employed by the transamidation pathway | Asn-tRNAAsn + ? | - |
? | |
6.3.5.6 | Asp-tRNAAsn + ? | Methanothermobacter thermautotrophicus | enzyme of the two-step pathway for the synthesis of Asn-tRNA that requires misacylated Asp-tRNAAsn as intermediate, which is generated by a Asp-tRNA synthetase | Asn-tRNAAsn + ? | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
6.1.1.12 | Ferroplasma acidarmanus | - |
- |
- |
6.1.1.12 | Methanothermobacter thermautotrophicus | - |
- |
- |
6.1.1.12 | Thermococcus kodakarensis | Q52428 | - |
- |
6.1.1.23 | Methanothermobacter thermautotrophicus | O26328 | - |
- |
6.1.1.23 | Methanothermobacter thermautotrophicus DSM 1053 | O26328 | - |
- |
6.3.5.6 | Methanothermobacter thermautotrophicus | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
6.1.1.12 | - |
Thermococcus kodakarensis |
6.1.1.12 | - |
Ferroplasma acidarmanus |
6.1.1.12 | recombinant His-tagged enzyme from Escherichia coli | Methanothermobacter thermautotrophicus |
6.1.1.12 | recombinant His-tagged enzyme from Escherichia coli | Thermococcus kodakarensis |
6.1.1.12 | recombinant His-tagged enzyme from Escherichia coli | Ferroplasma acidarmanus |
6.1.1.23 | - |
Methanothermobacter thermautotrophicus |
6.3.5.6 | purification of recombinant enzyme, expressed in Escherichia coli | Methanothermobacter thermautotrophicus |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
6.1.1.12 | ATP + L-aspartate + tRNAAsn | - |
Methanothermobacter thermautotrophicus | AMP + diphosphate + L-aspartyl-tRNAAsn | - |
? | |
6.1.1.12 | ATP + L-aspartate + tRNAAsn | the archaeal AspRS enzyme is nondiscriminating, which means that it forms Asp-tRNAAsp and Asp-tRNAAsn, which is the intermediate in AsntRNAAsn generation by ASp-tRNAAsn amidotransferase, in contrary bacterial enzymes are discriminating ones | Methanothermobacter thermautotrophicus | AMP + diphosphate + L-aspartyl-tRNAAsn | - |
? | |
6.1.1.12 | ATP + L-aspartate + tRNAAsp | - |
Methanothermobacter thermautotrophicus | AMP + diphosphate + L-aspartyl-tRNAAsp | - |
? | |
6.1.1.12 | ATP + L-aspartate + tRNAAsp | - |
Thermococcus kodakarensis | AMP + diphosphate + L-aspartyl-tRNAAsp | - |
? | |
6.1.1.12 | ATP + L-aspartate + tRNAAsp | - |
Ferroplasma acidarmanus | AMP + diphosphate + L-aspartyl-tRNAAsp | - |
? | |
6.1.1.12 | ATP + L-aspartate + tRNAAsp | no activity with tRNAAsn | Thermococcus kodakarensis | AMP + diphosphate + L-aspartyl-tRNAAsp | - |
? | |
6.1.1.12 | ATP + L-aspartate + tRNAAsp | no activity with tRNAAsn | Ferroplasma acidarmanus | AMP + diphosphate + L-aspartyl-tRNAAsp | - |
? | |
6.1.1.12 | additional information | no acylation with L-aspartate of tRNAAsn | Thermococcus kodakarensis | ? | - |
? | |
6.1.1.12 | additional information | no acylation with L-aspartate of tRNAAsn | Ferroplasma acidarmanus | ? | - |
? | |
6.1.1.12 | additional information | no acylation with L-aspartate of tRNAAsn, enzyme is discriminating, because it forms Asn-tRNAAsn by direct aminoacylation, not via the intermediate of Asp-tRNAAsn, and it contains no Asp-tRNAAsn amidotransferase | Thermococcus kodakarensis | ? | - |
? | |
6.1.1.12 | additional information | no acylation with L-aspartate of tRNAAsn, enzyme is discriminating, because it forms Asn-tRNAAsn by direct aminoacylation, not via the intermediate of Asp-tRNAAsn, and it contains no Asp-tRNAAsn amidotransferase | Ferroplasma acidarmanus | ? | - |
? | |
6.1.1.23 | ATP + L-aspartate + tRNAAsn | - |
Methanothermobacter thermautotrophicus | AMP + diphosphate + L-aspartyl-tRNAAsn | - |
? | |
6.1.1.23 | ATP + L-aspartate + tRNAAsn | - |
Methanothermobacter thermautotrophicus DSM 1053 | AMP + diphosphate + L-aspartyl-tRNAAsn | - |
? | |
6.1.1.23 | ATP + L-aspartate + tRNAAsp | - |
Methanothermobacter thermautotrophicus | AMP + diphosphate + L-aspartyl-tRNAAsp | - |
? | |
6.1.1.23 | ATP + L-aspartate + tRNAAsp | - |
Methanothermobacter thermautotrophicus DSM 1053 | AMP + diphosphate + L-aspartyl-tRNAAsp | - |
? | |
6.3.5.6 | Asp-tRNAAsn + ? | the mischarging Asp-tRNA synthetase correlates with the absence in the genome of Asn-tRNA synthetase and the presence of Asp-tRNAAsn amidotransferase, employed by the transamidation pathway | Methanothermobacter thermautotrophicus | Asn-tRNAAsn + ? | - |
? | |
6.3.5.6 | Asp-tRNAAsn + ? | enzyme of the two-step pathway for the synthesis of Asn-tRNA that requires misacylated Asp-tRNAAsn as intermediate, which is generated by a Asp-tRNA synthetase | Methanothermobacter thermautotrophicus | Asn-tRNAAsn + ? | - |
? | |
6.3.5.6 | ATP + Asp-tRNAAsn + glutamine | - |
Methanothermobacter thermautotrophicus | ADP + phosphate + Asn-tRNAAsn + glutamate | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
6.3.5.6 | heterotrimer | GatCAB enzyme | Methanothermobacter thermautotrophicus |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
6.1.1.12 | Aspartic acid translase | - |
Methanothermobacter thermautotrophicus |
6.1.1.12 | Aspartic acid translase | - |
Thermococcus kodakarensis |
6.1.1.12 | Aspartic acid translase | - |
Ferroplasma acidarmanus |
6.1.1.12 | Aspartyl ribonucleate synthetase | - |
Methanothermobacter thermautotrophicus |
6.1.1.12 | Aspartyl ribonucleate synthetase | - |
Thermococcus kodakarensis |
6.1.1.12 | Aspartyl ribonucleate synthetase | - |
Ferroplasma acidarmanus |
6.1.1.12 | aspartyl ribonuleic synthetase | - |
Methanothermobacter thermautotrophicus |
6.1.1.12 | aspartyl ribonuleic synthetase | - |
Thermococcus kodakarensis |
6.1.1.12 | aspartyl ribonuleic synthetase | - |
Ferroplasma acidarmanus |
6.1.1.12 | Aspartyl-transfer ribonucleic acid synthetase | - |
Methanothermobacter thermautotrophicus |
6.1.1.12 | Aspartyl-transfer ribonucleic acid synthetase | - |
Thermococcus kodakarensis |
6.1.1.12 | Aspartyl-transfer ribonucleic acid synthetase | - |
Ferroplasma acidarmanus |
6.1.1.12 | Aspartyl-transfer RNA synthetase | - |
Methanothermobacter thermautotrophicus |
6.1.1.12 | Aspartyl-transfer RNA synthetase | - |
Thermococcus kodakarensis |
6.1.1.12 | Aspartyl-transfer RNA synthetase | - |
Ferroplasma acidarmanus |
6.1.1.12 | Aspartyl-tRNA synthetase | - |
Methanothermobacter thermautotrophicus |
6.1.1.12 | Aspartyl-tRNA synthetase | - |
Thermococcus kodakarensis |
6.1.1.12 | Aspartyl-tRNA synthetase | - |
Ferroplasma acidarmanus |
6.1.1.12 | AspRS | - |
Methanothermobacter thermautotrophicus |
6.1.1.12 | discriminating aspartyl-tRNA synthetase | - |
Thermococcus kodakarensis |
6.1.1.12 | discriminating aspartyl-tRNA synthetase | - |
Ferroplasma acidarmanus |
6.1.1.23 | nondiscriminating aspartyl-tRNA synthetase | - |
Methanothermobacter thermautotrophicus |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
6.1.1.12 | 37 | - |
assay at | Ferroplasma acidarmanus |
6.1.1.12 | 60 | - |
- |
Thermococcus kodakarensis |
6.3.5.6 | 37 | - |
assay at, as a compromise between optimal enzyme activity and the stability of the reaction product | Methanothermobacter thermautotrophicus |
EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|---|
6.1.1.12 | 37 | 60 | - |
Methanothermobacter thermautotrophicus |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
6.1.1.12 | 6 | - |
- |
Thermococcus kodakarensis |
6.1.1.12 | 6 | - |
- |
Ferroplasma acidarmanus |
6.1.1.12 | 7 | - |
assay at | Methanothermobacter thermautotrophicus |