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Literature summary extracted from

  • Zhang, J.; Demain, A.L.
    In vitro stabilization of ACV synthetase activity from Streptomyces clavuligerus (1992), Appl. Biochem. Biotechnol., 37, 97-110.
No PubMed abstract available

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
6.3.2.26 Mg2+
-
 Streptomyces clavuligerus

Organic Solvent Stability

EC Number Organic Solvent Comment Organism
6.3.2.26 dithiothreitol stability of crude enzyme is increased by dithiothreitol Streptomyces clavuligerus

Organism

EC Number Organism UniProt Comment Textmining
6.3.2.26 Streptomyces clavuligerus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
6.3.2.26
-
 Streptomyces clavuligerus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.3.2.26 L-2-aminoadipate + L-cysteine + L-valine + ATP
-
 Streptomyces clavuligerus N-(5-amino-5-carboxypentanoyl)-L-cysteinyl-D-valine + AMP + diphosphate
-
 ?

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
6.3.2.26 4
-
-
 Streptomyces clavuligerus
6.3.2.26 25
-
 half of activity after 8 h Streptomyces clavuligerus
6.3.2.26 32
-
 half of activity after 10 min Streptomyces clavuligerus
6.3.2.26 34
-
 the mixture of ATP 5 mM, Mg2+ 5 mM, valine 5 mM prevents the thermal inactivation, 92% activity after 10 min Streptomyces clavuligerus
6.3.2.26 34
-
 the mixture of ATP 5 mM, DDT 3 mM, Mg2+ 5 mM, aminoadipate 5 mM, cysteine 1 mM and valine 5 mM prevents the thermal inactivation, 91% activity after 10 min Streptomyces clavuligerus

Cofactor

EC Number Cofactor Comment Organism Structure
6.3.2.26 ATP
-
 Streptomyces clavuligerus