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Literature summary extracted from

  • Peters, R.J.; Flory, J.E.; Jetter, R.; Ravn, M.M.; Lee, H.J.; Coates, R.M.; Croteau, R.B.
    Abietadiene synthase from grand fir (Abies grandis): characterization and mechanism of action of the "pseudomature" recombinant enzyme (2000), Biochemistry, 39, 15592-15602.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.2.3.18 expression in Escherichia coli Abies grandis
4.2.3.132 truncated abietadiene synthase is expressed in Escherichia coli BL21(DE3) cells Abies grandis
5.5.1.12 expression in Escherichia coli Abies grandis

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.2.3.18 geranylgeranyl diphosphate substrate inhibition Abies grandis
5.5.1.12 geranylgeranyl diphosphate substrate inhibition Abies grandis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.2.3.18 0.00035
-
 copalyl diphosphate
-
 Abies grandis
4.2.3.132 0.00035
-
 (+)-copalyl diphosphate in 50 mM HEPES, pH 7.2, 100 mM KCl, 7.5 mM MgCl2, 0.02 mM MnCl2, at 30°C Abies grandis
5.5.1.12 0.003
-
 geranylgeranyl diphosphate
-
 Abies grandis

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
4.2.3.18 soluble
-
 Abies grandis
-
-
5.5.1.12 soluble
-
 Abies grandis
-
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.2.3.18 80000
-
 gel filtration Abies grandis
5.5.1.12 80000
-
-
 Abies grandis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.2.3.18 (+)-copalyl-diphosphate Abies grandis
-
 (-)-abietadiene + diphosphate
-
 ?
5.5.1.12 geranylgeranyl diphosphate Abies grandis
-
 (+)-copalyl diphosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
4.2.3.18 Abies grandis
-
-
-
4.2.3.132 Abies grandis
-
 grand fir
-
5.5.1.12 Abies grandis
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.2.3.18
-
 Abies grandis
4.2.3.132 type II ceramic hydroxyapatite column chromatography and POROS HQ/M column chromatography Abies grandis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.3.18 (+)-copalyl-diphosphate
-
 Abies grandis (-)-abietadiene + diphosphate
-
 ?
4.2.3.132 (+)-copalyl diphosphate the enzyme converts geranylgeranyl diphosphate and the intermediate (+)-copalyl diphosphate to a nearly equal mixture of abietadiene (31%), levopimaradiene (34%), and neoabietadiene (28%), as well as to three minor products pimara-8(14),15-diene (3%), palustradiene (2%), and sandaracopimaradiene (2%) Abies grandis ?
-
 ?
5.5.1.12 farnesyl diphosphate
-
 Abies grandis (E)-beta-farnesene
-
 ?
5.5.1.12 geranylgeranyl diphosphate
-
 Abies grandis (+)-copalyl diphosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
4.2.3.18 monomer 1 * 80000, SDS-PAGE Abies grandis
5.5.1.12 monomer
-
 Abies grandis

Synonyms

EC Number Synonyms Comment Organism
4.2.3.132 abietadiene synthase cf. EC 4.2.3.18 Abies grandis
4.2.3.132 NAS
-
 Abies grandis

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
4.2.3.18 0.75
-
 copalyl diphosphate
-
 Abies grandis
4.2.3.132 0.75
-
 (+)-copalyl diphosphate in 50 mM HEPES, pH 7.2, 100 mM KCl, 7.5 mM MgCl2, 0.02 mM MnCl2, at 30°C Abies grandis
5.5.1.12 0.02
-
 farnesyl diphosphate
-
 Abies grandis
5.5.1.12 0.75
-
 geranylgeranyl diphosphate
-
 Abies grandis

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
4.2.3.132 2000
-
 (+)-copalyl diphosphate in 50 mM HEPES, pH 7.2, 100 mM KCl, 7.5 mM MgCl2, 0.02 mM MnCl2, at 30°C Abies grandis