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Literature summary extracted from

  • Caldwell, C.R.; Turano, F.J.; McMahon, M.B.
    Identification of two cytosolic ascorbate peroxidase cDNAs from soybean leaves and characterization of their products by functional expression in E. coli (1998), Planta, 204, 120-126.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.11.1.11 expression in Escherichia coli of two soybean ascorbate peroxidase cDNAs Glycine max

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.11.1.11 C2H2 recombinant enzyme 1: 94% inhibition at 0.1 ml per l, recombinant enzyme 2: 2% inhibition at 0.1 ml per l Glycine max
1.11.1.11 hydroxylamine recombinant enzyme 1: 74% inhibition at 1 mM and 100% inhibition at 10 mM, recombinant enzyme 2: 86% inhibition at 1 mM and 100% inhibition at 10 mM Glycine max
1.11.1.11 KCN recombinant enzyme 1: 69% inhibition at 0.1 mM and 100% inhibition at 0.5 mM, recombinant enzyme 2: 81% inhibition at 0.1 mM and 100% inhibition at 0.5 mM Glycine max
1.11.1.11 p-chloromercuriphenyl sulfonic acid 100% inhibition at 0.05 mM, recombinant enzyme 1 and 2 Glycine max

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.11.1.11 0.011
-
H2O2 recombinant enzyme 1 Glycine max
1.11.1.11 0.024
-
H2O2 recombinant enzyme 2 Glycine max
1.11.1.11 0.333
-
ascorbate recombinant enzyme 1 Glycine max
1.11.1.11 0.467
-
ascorbate recombinant enzyme 2 Glycine max

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.11.1.11 cytosol presence of two major non-plastid isozymes Glycine max 5829
-
1.11.1.11 additional information the doublet in cytosolic enzyme activity observed in the native-PAGE of soybean leaf and root nodule extracts could represent either the products of two cytosolic soybean enzyme genes or a single gene product with some post-translational modification, the cultivar Roanoke has a single isozyme Glycine max
-
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.11.1.11 27000
-
recombinant enzyme 1 and 2, calculated from sequence of cDNA Glycine max
1.11.1.11 28000
-
x * 28000, SDS-PAGE, recombinant enzyme 2 Glycine max
1.11.1.11 30000
-
x * 30000, SDS-PAGE, recombinant enzyme 1 Glycine max
1.11.1.11 46000
-
recombinant enzyme 2, column chromatography in the absence of denaturant Glycine max
1.11.1.11 48000
-
recombinant enzyme 1, column chromatography in the absence of denaturant Glycine max

Organism

EC Number Organism UniProt Comment Textmining
1.11.1.11 Glycine max
-
soybean
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.11.1.11 of recombinant enzyme 1: using column chromatography on DEAE Bio-Gel A agarose and column chromatography on Sephacryl S-300, of recombinant enzyme 2: using column chromatography on DEAE Bio-Gel A agarose, ammonium sulfate fractionation, dialysis, chromatofocusing on a polybuffer 94 exchanger and column chromatography on Sephacryl S-300 Glycine max

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.11.1.11 hypocotyl presence of two major non-plastid isozymes Glycine max
-
1.11.1.11 leaf presence of two major non-plastid isozymes Glycine max
-
1.11.1.11 root nodules Glycine max
-
1.11.1.11 root presence of two major non-plastid isozymes Glycine max
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.11.1.11 9.4
-
recombinant enzyme 1, after DEAE-column chromatography Glycine max
1.11.1.11 63.6
-
recombinant enzyme 2, after chromatofocusing Glycine max

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.11.1.11 guaiacol + H2O2 recombinant enzyme 1: 6% activity relative to L-ascorbate, recombinant enzyme 2: 11% relative activity to L-ascorbate Glycine max ?
-
?
1.11.1.11 L-ascorbate + H2O2
-
Glycine max dehydroascorbate + H2O
-
?
1.11.1.11 pyrogallol + H2O2 recombinant enzyme 1: 355% activity relative to L-ascorbate, recombinant enzyme 2: 304% activity relative to L-ascorbate Glycine max 3-hydroxybenzo-1,2-quinone + H2O
-
?

Subunits

EC Number Subunits Comment Organism
1.11.1.11 ? x * 30000, SDS-PAGE, recombinant enzyme 1 Glycine max
1.11.1.11 ? x * 28000, SDS-PAGE, recombinant enzyme 2 Glycine max

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.11.1.11 6.6
-
recombinant enzyme 2 Glycine max
1.11.1.11 7
-
assay at Glycine max
1.11.1.11 7
-
recombinant enzyme 1 Glycine max