EC Number | General Stability | Organism |
---|---|---|
1.11.1.11 | dialysis of crude enzyme preparations for 24 h against a buffer depleted of ascorbate or sorbitol results in 90% and 20% loss in enzyme activity, respectively. In air saturated solutions, salting-out results in a significant loss in enzyme activity, therefore exchange to N2 gas is performed prior to salting-out of the root enzyme | Raphanus sativus |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
1.11.1.11 | 2-mercaptoethanol | not inhibitory at 0.5 mM, 31% inhibition at 5 mM | Raphanus sativus | |
1.11.1.11 | 5,5'-dithiobis(2-nitrobenzoic acid) | 40% inhibition at 0.1 mM | Raphanus sativus | |
1.11.1.11 | cysteine | 50% inhibition at 5 mM | Raphanus sativus | |
1.11.1.11 | dithioerythritol | 67% inhibition at 0.05 mM | Raphanus sativus | |
1.11.1.11 | dithiothreitol | 54% inhibition at 0.05 mM | Raphanus sativus | |
1.11.1.11 | EDTA | not inhibitory | Raphanus sativus | |
1.11.1.11 | iodoacetate | not inhibitory | Raphanus sativus | |
1.11.1.11 | KCN | 95% inhibition at 0.1 mM | Raphanus sativus | |
1.11.1.11 | NaN3 | 17% inhibition at 1 mM, 87% inhibition at 10 mM | Raphanus sativus | |
1.11.1.11 | p-chloromercuribenzoate | 87% inhibition at 0.005 mM, inactivation is partially reversible, 2-mercaptoethanol protects | Raphanus sativus | |
1.11.1.11 | reduced glutathione | 33% inhibition at 5 mM | Raphanus sativus |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.11.1.11 | 0.13 | - |
H2O2 | - |
Raphanus sativus | |
1.11.1.11 | 0.77 | - |
ascorbate | - |
Raphanus sativus |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
1.11.1.11 | cytosol | - |
Raphanus sativus | 5829 | - |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
1.11.1.11 | Fe | - |
Raphanus sativus |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
1.11.1.11 | 28000 | - |
native PAGE | Raphanus sativus |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.11.1.11 | Raphanus sativus | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.11.1.11 | using ammonium sulfate precipitation, dialysis and column chromatography on butyl-Toyopearl, DEAE-cellulofine and Sephadex G-75 | Raphanus sativus |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
1.11.1.11 | root | - |
Raphanus sativus | - |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
1.11.1.11 | 561.1 | - |
purified enzyme | Raphanus sativus |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.11.1.11 | cytochrome c + H2O2 | no activity | Raphanus sativus | ? + H2O | - |
? | |
1.11.1.11 | guaiacol + H2O2 | no activity | Raphanus sativus | ? | - |
? | |
1.11.1.11 | L-ascorbate + H2O2 | highly specific for L-ascorbate | Raphanus sativus | dehydroascorbate + H2O | - |
? | |
1.11.1.11 | additional information | no activity with: cytochrome c, reduced glutathione, NADH, NADPH, 6-palmityl-ascorbate, ascorbate-2-sulfate, guaiacol, 3,3'-diaminobenzidine, pyrocatechol or D-iso-ascorbate | Raphanus sativus | ? | - |
? | |
1.11.1.11 | o-dianisidine + H2O2 | the oxidation rate is only 8.6% of that with L-ascorbate | Raphanus sativus | ? | - |
? | |
1.11.1.11 | pyrogallol + H2O2 | the oxidation rate is only 5.5% of that with L-ascorbate | Raphanus sativus | 3-hydroxybenzo-1,2-quinone + H2O | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
1.11.1.11 | monomer | 1 * 28000, SDS-PAGE | Raphanus sativus |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
1.11.1.11 | 6 | - |
- |
Raphanus sativus |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.11.1.11 | heme | - |
Raphanus sativus |