EC Number | Activating Compound | Comment | Organism | Structure |
---|---|---|---|---|
1.11.1.13 | malonate | stabilizes Mn3+ as chelator | Phanerodontia chrysosporium | |
1.11.1.13 | oxalate | potent chelator of Mn3+ | Phanerodontia chrysosporium |
EC Number | Crystallization (Comment) | Organism |
---|---|---|
1.11.1.13 | crystal structure | Phanerodontia chrysosporium |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
1.11.1.13 | Cd2+ | competitive inhibitor to Mn2+, uncompetitive to H2O2, reversibly inhibits oxidation of Mn2+ and Mn3+-mediated oxidation of 2,6-dimethoxyphenol, but not oxidation of phenols in absence of Mn2+, Cd2+ inhibits reduction of compound I and II by Mn2+ at pH 4.5 and binds at the Mn2+-binding site, kinetics of inhibition | Phanerodontia chrysosporium | |
1.11.1.13 | Co2+ | competitive inhibitor to Mn2+ | Phanerodontia chrysosporium | |
1.11.1.13 | Eu3+ | competitive inhibitor to Mn2+ | Phanerodontia chrysosporium | |
1.11.1.13 | additional information | not inhibited by Ca2+ and Mg2+ | Phanerodontia chrysosporium | |
1.11.1.13 | Sm3+ | competitive inhibitor to Mn2+ | Phanerodontia chrysosporium |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
1.11.1.13 | extracellular | - |
Phanerodontia chrysosporium | - |
- |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.11.1.13 | Mn2+ + H+ + H2O2 | Phanerodontia chrysosporium | Mn2+ is a component of woody plant tissues | Mn3+ + H2O | - |
? | |
1.11.1.13 | Mn2+ + H+ + H2O2 | Phanerodontia chrysosporium | important component of lignin degradation system | Mn3+ + H2O | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.11.1.13 | Phanerodontia chrysosporium | - |
- |
- |
1.11.1.13 | Phanerodontia chrysosporium | - |
white rot basidomycete | - |
1.11.1.13 | Phanerodontia chrysosporium | - |
MnP1 | - |
1.11.1.13 | Phanerodontia chrysosporium OGC101 | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.11.1.13 | isoenzyme 1 | Phanerodontia chrysosporium |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.11.1.13 | Mn2+ + H+ + H2O2 | unique binding and oxidation site for Mn2+, single Mn atom is hexacoordinate, with two water ligands and four carboxylate ligands from heme propionate 6 and amino acids Glu-35, Glu-39 and Asp-179 | Phanerodontia chrysosporium | Mn3+ + H2O | Mn3+ oxidizes phenolic lignin model compounds | ? | |
1.11.1.13 | Mn2+ + H+ + H2O2 | unique binding and oxidation site for Mn2+, single Mn atom is hexacoordinate, with two water ligands and four carboxylate ligands from heme propionate 6 and amino acids Glu-35, Glu-39 and Asp-179 | Phanerodontia chrysosporium | Mn3+ + H2O | Mn3+ oxidizes lignin | ? | |
1.11.1.13 | Mn2+ + H+ + H2O2 | unique binding and oxidation site for Mn2+, single Mn atom is hexacoordinate, with two water ligands and four carboxylate ligands from heme propionate 6 and amino acids Glu-35, Glu-39 and Asp-179 | Phanerodontia chrysosporium | Mn3+ + H2O | Mn3+ oxidizes a variety of phenols | ? | |
1.11.1.13 | Mn2+ + H+ + H2O2 | Mn2+ is a component of woody plant tissues | Phanerodontia chrysosporium | Mn3+ + H2O | - |
? | |
1.11.1.13 | Mn2+ + H+ + H2O2 | important component of lignin degradation system | Phanerodontia chrysosporium | Mn3+ + H2O | - |
? | |
1.11.1.13 | Mn2+ + H+ + H2O2 | unique binding and oxidation site for Mn2+, single Mn atom is hexacoordinate, with two water ligands and four carboxylate ligands from heme propionate 6 and amino acids Glu-35, Glu-39 and Asp-179 | Phanerodontia chrysosporium OGC101 | Mn3+ + H2O | Mn3+ oxidizes phenolic lignin model compounds | ? | |
1.11.1.13 | Mn2+ + H+ + H2O2 | unique binding and oxidation site for Mn2+, single Mn atom is hexacoordinate, with two water ligands and four carboxylate ligands from heme propionate 6 and amino acids Glu-35, Glu-39 and Asp-179 | Phanerodontia chrysosporium OGC101 | Mn3+ + H2O | Mn3+ oxidizes lignin | ? | |
1.11.1.13 | Mn2+ + H+ + H2O2 | unique binding and oxidation site for Mn2+, single Mn atom is hexacoordinate, with two water ligands and four carboxylate ligands from heme propionate 6 and amino acids Glu-35, Glu-39 and Asp-179 | Phanerodontia chrysosporium OGC101 | Mn3+ + H2O | Mn3+ oxidizes a variety of phenols | ? | |
1.11.1.13 | additional information | catalytic cycle with oxidized intermediates MnP compound I and II | Phanerodontia chrysosporium | ? | - |
? | |
1.11.1.13 | additional information | Mn2+-independent oxidation of small phenolic compounds, such as guaiacol and dimethoxyphenol, rates are greatly reduced compared with the Mn-mediated reaction | Phanerodontia chrysosporium | ? | - |
? | |
1.11.1.13 | additional information | catalytic cycle with oxidized intermediates MnP compound I and II | Phanerodontia chrysosporium OGC101 | ? | - |
? | |
1.11.1.13 | additional information | Mn2+-independent oxidation of small phenolic compounds, such as guaiacol and dimethoxyphenol, rates are greatly reduced compared with the Mn-mediated reaction | Phanerodontia chrysosporium OGC101 | ? | - |
? |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.11.1.13 | 55 | - |
pH 4.5, 1.4 min, 50% loss of activity, both Mn2+ and Cd2+ protect MnP from thermal denaturation more efficiently than Ca2+ at pH 4.5, extending the half-life more than 2fold at 1 mM, combination of 0.5 mM Mn2+ and 0.5 mM Cd2+ extends the half-life more than 10fold | Phanerodontia chrysosporium |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.11.1.13 | additional information | - |
additional information | - |
Phanerodontia chrysosporium |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
1.11.1.13 | 4.5 | - |
- |
Phanerodontia chrysosporium |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.11.1.13 | heme | heme protein | Phanerodontia chrysosporium |
EC Number | Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.11.1.13 | additional information | - |
additional information | transient-state inhibition constants | Phanerodontia chrysosporium | |
1.11.1.13 | 0.004 | - |
Cd2+ | oxidation of 2,6-dimethoxyphenol | Phanerodontia chrysosporium | |
1.11.1.13 | 0.01 | - |
Cd2+ | oxidation of Mn2+ | Phanerodontia chrysosporium | |
1.11.1.13 | 1 | - |
Co2+ | - |
Phanerodontia chrysosporium |