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Literature summary extracted from

  • Youngs, H.L.; Sundaramoorthy, M; Gold, M.H.
    Effects of cadmium on manganese peroxidase. Competitive inhibition of MnII oxidation and thermal stabilization of the enzyme (2000), Eur. J. Biochem., 267, 1761-1769.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.11.1.13 malonate stabilizes Mn3+ as chelator Phanerodontia chrysosporium
1.11.1.13 oxalate potent chelator of Mn3+ Phanerodontia chrysosporium

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
1.11.1.13 crystal structure Phanerodontia chrysosporium

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.11.1.13 Cd2+ competitive inhibitor to Mn2+, uncompetitive to H2O2, reversibly inhibits oxidation of Mn2+ and Mn3+-mediated oxidation of 2,6-dimethoxyphenol, but not oxidation of phenols in absence of Mn2+, Cd2+ inhibits reduction of compound I and II by Mn2+ at pH 4.5 and binds at the Mn2+-binding site, kinetics of inhibition Phanerodontia chrysosporium
1.11.1.13 Co2+ competitive inhibitor to Mn2+ Phanerodontia chrysosporium
1.11.1.13 Eu3+ competitive inhibitor to Mn2+ Phanerodontia chrysosporium
1.11.1.13 additional information not inhibited by Ca2+ and Mg2+ Phanerodontia chrysosporium
1.11.1.13 Sm3+ competitive inhibitor to Mn2+ Phanerodontia chrysosporium

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.11.1.13 extracellular
-
Phanerodontia chrysosporium
-
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.11.1.13 Mn2+ + H+ + H2O2 Phanerodontia chrysosporium Mn2+ is a component of woody plant tissues Mn3+ + H2O
-
?
1.11.1.13 Mn2+ + H+ + H2O2 Phanerodontia chrysosporium important component of lignin degradation system Mn3+ + H2O
-
?

Organism

EC Number Organism UniProt Comment Textmining
1.11.1.13 Phanerodontia chrysosporium
-
-
-
1.11.1.13 Phanerodontia chrysosporium
-
white rot basidomycete
-
1.11.1.13 Phanerodontia chrysosporium
-
MnP1
-
1.11.1.13 Phanerodontia chrysosporium OGC101
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.11.1.13 isoenzyme 1 Phanerodontia chrysosporium

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.11.1.13 Mn2+ + H+ + H2O2 unique binding and oxidation site for Mn2+, single Mn atom is hexacoordinate, with two water ligands and four carboxylate ligands from heme propionate 6 and amino acids Glu-35, Glu-39 and Asp-179 Phanerodontia chrysosporium Mn3+ + H2O Mn3+ oxidizes phenolic lignin model compounds ?
1.11.1.13 Mn2+ + H+ + H2O2 unique binding and oxidation site for Mn2+, single Mn atom is hexacoordinate, with two water ligands and four carboxylate ligands from heme propionate 6 and amino acids Glu-35, Glu-39 and Asp-179 Phanerodontia chrysosporium Mn3+ + H2O Mn3+ oxidizes lignin ?
1.11.1.13 Mn2+ + H+ + H2O2 unique binding and oxidation site for Mn2+, single Mn atom is hexacoordinate, with two water ligands and four carboxylate ligands from heme propionate 6 and amino acids Glu-35, Glu-39 and Asp-179 Phanerodontia chrysosporium Mn3+ + H2O Mn3+ oxidizes a variety of phenols ?
1.11.1.13 Mn2+ + H+ + H2O2 Mn2+ is a component of woody plant tissues Phanerodontia chrysosporium Mn3+ + H2O
-
?
1.11.1.13 Mn2+ + H+ + H2O2 important component of lignin degradation system Phanerodontia chrysosporium Mn3+ + H2O
-
?
1.11.1.13 Mn2+ + H+ + H2O2 unique binding and oxidation site for Mn2+, single Mn atom is hexacoordinate, with two water ligands and four carboxylate ligands from heme propionate 6 and amino acids Glu-35, Glu-39 and Asp-179 Phanerodontia chrysosporium OGC101 Mn3+ + H2O Mn3+ oxidizes phenolic lignin model compounds ?
1.11.1.13 Mn2+ + H+ + H2O2 unique binding and oxidation site for Mn2+, single Mn atom is hexacoordinate, with two water ligands and four carboxylate ligands from heme propionate 6 and amino acids Glu-35, Glu-39 and Asp-179 Phanerodontia chrysosporium OGC101 Mn3+ + H2O Mn3+ oxidizes lignin ?
1.11.1.13 Mn2+ + H+ + H2O2 unique binding and oxidation site for Mn2+, single Mn atom is hexacoordinate, with two water ligands and four carboxylate ligands from heme propionate 6 and amino acids Glu-35, Glu-39 and Asp-179 Phanerodontia chrysosporium OGC101 Mn3+ + H2O Mn3+ oxidizes a variety of phenols ?
1.11.1.13 additional information catalytic cycle with oxidized intermediates MnP compound I and II Phanerodontia chrysosporium ?
-
?
1.11.1.13 additional information Mn2+-independent oxidation of small phenolic compounds, such as guaiacol and dimethoxyphenol, rates are greatly reduced compared with the Mn-mediated reaction Phanerodontia chrysosporium ?
-
?
1.11.1.13 additional information catalytic cycle with oxidized intermediates MnP compound I and II Phanerodontia chrysosporium OGC101 ?
-
?
1.11.1.13 additional information Mn2+-independent oxidation of small phenolic compounds, such as guaiacol and dimethoxyphenol, rates are greatly reduced compared with the Mn-mediated reaction Phanerodontia chrysosporium OGC101 ?
-
?

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.11.1.13 55
-
pH 4.5, 1.4 min, 50% loss of activity, both Mn2+ and Cd2+ protect MnP from thermal denaturation more efficiently than Ca2+ at pH 4.5, extending the half-life more than 2fold at 1 mM, combination of 0.5 mM Mn2+ and 0.5 mM Cd2+ extends the half-life more than 10fold Phanerodontia chrysosporium

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.11.1.13 additional information
-
additional information
-
Phanerodontia chrysosporium

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.11.1.13 4.5
-
-
Phanerodontia chrysosporium

Cofactor

EC Number Cofactor Comment Organism Structure
1.11.1.13 heme heme protein Phanerodontia chrysosporium

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1.11.1.13 additional information
-
additional information transient-state inhibition constants Phanerodontia chrysosporium
1.11.1.13 0.004
-
Cd2+ oxidation of 2,6-dimethoxyphenol Phanerodontia chrysosporium
1.11.1.13 0.01
-
Cd2+ oxidation of Mn2+ Phanerodontia chrysosporium
1.11.1.13 1
-
Co2+
-
Phanerodontia chrysosporium