BRENDA - Enzyme Database

Effects of cadmium on manganese peroxidase. Competitive inhibition of MnII oxidation and thermal stabilization of the enzyme

Youngs, H.L.; Sundaramoorthy, M; Gold, M.H.; Eur. J. Biochem. 267, 1761-1769 (2000)

Data extracted from this reference:

Activating Compound
EC Number
Activating Compound
Commentary
Organism
Structure
1.11.1.13
malonate
stabilizes Mn3+ as chelator
Phanerochaete chrysosporium
1.11.1.13
oxalate
potent chelator of Mn3+
Phanerochaete chrysosporium
Crystallization (Commentary)
EC Number
Crystallization
Organism
1.11.1.13
crystal structure
Phanerochaete chrysosporium
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
1.11.1.13
Cd2+
competitive inhibitor to Mn2+, uncompetitive to H2O2, reversibly inhibits oxidation of Mn2+ and Mn3+-mediated oxidation of 2,6-dimethoxyphenol, but not oxidation of phenols in absence of Mn2+, Cd2+ inhibits reduction of compound I and II by Mn2+ at pH 4.5 and binds at the Mn2+-binding site, kinetics of inhibition
Phanerochaete chrysosporium
1.11.1.13
Co2+
competitive inhibitor to Mn2+
Phanerochaete chrysosporium
1.11.1.13
Eu3+
competitive inhibitor to Mn2+
Phanerochaete chrysosporium
1.11.1.13
additional information
not inhibited by Ca2+ and Mg2+
Phanerochaete chrysosporium
1.11.1.13
Sm3+
competitive inhibitor to Mn2+
Phanerochaete chrysosporium
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
1.11.1.13
extracellular
-
Phanerochaete chrysosporium
-
-
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.11.1.13
Mn2+ + H+ + H2O2
Phanerochaete chrysosporium
Mn2+ is a component of woody plant tissues
Mn3+ + H2O
-
Phanerochaete chrysosporium
?
1.11.1.13
Mn2+ + H+ + H2O2
Phanerochaete chrysosporium
important component of lignin degradation system
Mn3+ + H2O
-
Phanerochaete chrysosporium
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
1.11.1.13
Phanerochaete chrysosporium
-
; MnP1; white rot basidomycete
-
1.11.1.13
Phanerochaete chrysosporium OGC101
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
1.11.1.13
isoenzyme 1
Phanerochaete chrysosporium
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.11.1.13
Mn2+ + H+ + H2O2
unique binding and oxidation site for Mn2+, single Mn atom is hexacoordinate, with two water ligands and four carboxylate ligands from heme propionate 6 and amino acids Glu-35, Glu-39 and Asp-179
439854
Phanerochaete chrysosporium
Mn3+ + H2O
Mn3+ oxidizes phenolic lignin model compounds
439854
Phanerochaete chrysosporium
-
1.11.1.13
Mn2+ + H+ + H2O2
unique binding and oxidation site for Mn2+, single Mn atom is hexacoordinate, with two water ligands and four carboxylate ligands from heme propionate 6 and amino acids Glu-35, Glu-39 and Asp-179
439854
Phanerochaete chrysosporium
Mn3+ + H2O
Mn3+ oxidizes lignin
439854
Phanerochaete chrysosporium
-
1.11.1.13
Mn2+ + H+ + H2O2
unique binding and oxidation site for Mn2+, single Mn atom is hexacoordinate, with two water ligands and four carboxylate ligands from heme propionate 6 and amino acids Glu-35, Glu-39 and Asp-179
439854
Phanerochaete chrysosporium
Mn3+ + H2O
Mn3+ oxidizes a variety of phenols
439854
Phanerochaete chrysosporium
-
1.11.1.13
Mn2+ + H+ + H2O2
Mn2+ is a component of woody plant tissues
439854
Phanerochaete chrysosporium
Mn3+ + H2O
-
439854
Phanerochaete chrysosporium
?
1.11.1.13
Mn2+ + H+ + H2O2
important component of lignin degradation system
439854
Phanerochaete chrysosporium
Mn3+ + H2O
-
439854
Phanerochaete chrysosporium
?
1.11.1.13
Mn2+ + H+ + H2O2
unique binding and oxidation site for Mn2+, single Mn atom is hexacoordinate, with two water ligands and four carboxylate ligands from heme propionate 6 and amino acids Glu-35, Glu-39 and Asp-179
439854
Phanerochaete chrysosporium OGC101
Mn3+ + H2O
Mn3+ oxidizes phenolic lignin model compounds
439854
Phanerochaete chrysosporium OGC101
-
1.11.1.13
Mn2+ + H+ + H2O2
unique binding and oxidation site for Mn2+, single Mn atom is hexacoordinate, with two water ligands and four carboxylate ligands from heme propionate 6 and amino acids Glu-35, Glu-39 and Asp-179
439854
Phanerochaete chrysosporium OGC101
Mn3+ + H2O
Mn3+ oxidizes lignin
439854
Phanerochaete chrysosporium OGC101
-
1.11.1.13
Mn2+ + H+ + H2O2
unique binding and oxidation site for Mn2+, single Mn atom is hexacoordinate, with two water ligands and four carboxylate ligands from heme propionate 6 and amino acids Glu-35, Glu-39 and Asp-179
439854
Phanerochaete chrysosporium OGC101
Mn3+ + H2O
Mn3+ oxidizes a variety of phenols
439854
Phanerochaete chrysosporium OGC101
-
1.11.1.13
additional information
catalytic cycle with oxidized intermediates MnP compound I and II
439854
Phanerochaete chrysosporium
?
-
-
-
-
1.11.1.13
additional information
Mn2+-independent oxidation of small phenolic compounds, such as guaiacol and dimethoxyphenol, rates are greatly reduced compared with the Mn-mediated reaction
439854
Phanerochaete chrysosporium
?
-
-
-
-
1.11.1.13
additional information
catalytic cycle with oxidized intermediates MnP compound I and II
439854
Phanerochaete chrysosporium OGC101
?
-
-
-
-
1.11.1.13
additional information
Mn2+-independent oxidation of small phenolic compounds, such as guaiacol and dimethoxyphenol, rates are greatly reduced compared with the Mn-mediated reaction
439854
Phanerochaete chrysosporium OGC101
?
-
-
-
-
Temperature Stability [°C]
EC Number
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
1.11.1.13
55
-
pH 4.5, 1.4 min, 50% loss of activity, both Mn2+ and Cd2+ protect MnP from thermal denaturation more efficiently than Ca2+ at pH 4.5, extending the half-life more than 2fold at 1 mM, combination of 0.5 mM Mn2+ and 0.5 mM Cd2+ extends the half-life more than 10fold
Phanerochaete chrysosporium
Turnover Number [1/s]
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
1.11.1.13
additional information
-
additional information
-
Phanerochaete chrysosporium
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.11.1.13
4.5
-
-
Phanerochaete chrysosporium
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
1.11.1.13
heme
heme protein
Phanerochaete chrysosporium
Ki Value [mM]
EC Number
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
1.11.1.13
additional information
-
additional information
transient-state inhibition constants
Phanerochaete chrysosporium
1.11.1.13
0.004
-
Cd2+
oxidation of 2,6-dimethoxyphenol
Phanerochaete chrysosporium
1.11.1.13
0.01
-
Cd2+
oxidation of Mn2+
Phanerochaete chrysosporium
1.11.1.13
1
-
Co2+
-
Phanerochaete chrysosporium
Activating Compound (protein specific)
EC Number
Activating Compound
Commentary
Organism
Structure
1.11.1.13
malonate
stabilizes Mn3+ as chelator
Phanerochaete chrysosporium
1.11.1.13
oxalate
potent chelator of Mn3+
Phanerochaete chrysosporium
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
1.11.1.13
heme
heme protein
Phanerochaete chrysosporium
Crystallization (Commentary) (protein specific)
EC Number
Crystallization
Organism
1.11.1.13
crystal structure
Phanerochaete chrysosporium
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
1.11.1.13
Cd2+
competitive inhibitor to Mn2+, uncompetitive to H2O2, reversibly inhibits oxidation of Mn2+ and Mn3+-mediated oxidation of 2,6-dimethoxyphenol, but not oxidation of phenols in absence of Mn2+, Cd2+ inhibits reduction of compound I and II by Mn2+ at pH 4.5 and binds at the Mn2+-binding site, kinetics of inhibition
Phanerochaete chrysosporium
1.11.1.13
Co2+
competitive inhibitor to Mn2+
Phanerochaete chrysosporium
1.11.1.13
Eu3+
competitive inhibitor to Mn2+
Phanerochaete chrysosporium
1.11.1.13
additional information
not inhibited by Ca2+ and Mg2+
Phanerochaete chrysosporium
1.11.1.13
Sm3+
competitive inhibitor to Mn2+
Phanerochaete chrysosporium
Ki Value [mM] (protein specific)
EC Number
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
1.11.1.13
additional information
-
additional information
transient-state inhibition constants
Phanerochaete chrysosporium
1.11.1.13
0.004
-
Cd2+
oxidation of 2,6-dimethoxyphenol
Phanerochaete chrysosporium
1.11.1.13
0.01
-
Cd2+
oxidation of Mn2+
Phanerochaete chrysosporium
1.11.1.13
1
-
Co2+
-
Phanerochaete chrysosporium
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
1.11.1.13
extracellular
-
Phanerochaete chrysosporium
-
-
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.11.1.13
Mn2+ + H+ + H2O2
Phanerochaete chrysosporium
Mn2+ is a component of woody plant tissues
Mn3+ + H2O
-
Phanerochaete chrysosporium
?
1.11.1.13
Mn2+ + H+ + H2O2
Phanerochaete chrysosporium
important component of lignin degradation system
Mn3+ + H2O
-
Phanerochaete chrysosporium
?
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
1.11.1.13
isoenzyme 1
Phanerochaete chrysosporium
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.11.1.13
Mn2+ + H+ + H2O2
unique binding and oxidation site for Mn2+, single Mn atom is hexacoordinate, with two water ligands and four carboxylate ligands from heme propionate 6 and amino acids Glu-35, Glu-39 and Asp-179
439854
Phanerochaete chrysosporium
Mn3+ + H2O
Mn3+ oxidizes phenolic lignin model compounds
439854
Phanerochaete chrysosporium
-
1.11.1.13
Mn2+ + H+ + H2O2
unique binding and oxidation site for Mn2+, single Mn atom is hexacoordinate, with two water ligands and four carboxylate ligands from heme propionate 6 and amino acids Glu-35, Glu-39 and Asp-179
439854
Phanerochaete chrysosporium
Mn3+ + H2O
Mn3+ oxidizes lignin
439854
Phanerochaete chrysosporium
-
1.11.1.13
Mn2+ + H+ + H2O2
unique binding and oxidation site for Mn2+, single Mn atom is hexacoordinate, with two water ligands and four carboxylate ligands from heme propionate 6 and amino acids Glu-35, Glu-39 and Asp-179
439854
Phanerochaete chrysosporium
Mn3+ + H2O
Mn3+ oxidizes a variety of phenols
439854
Phanerochaete chrysosporium
-
1.11.1.13
Mn2+ + H+ + H2O2
Mn2+ is a component of woody plant tissues
439854
Phanerochaete chrysosporium
Mn3+ + H2O
-
439854
Phanerochaete chrysosporium
?
1.11.1.13
Mn2+ + H+ + H2O2
important component of lignin degradation system
439854
Phanerochaete chrysosporium
Mn3+ + H2O
-
439854
Phanerochaete chrysosporium
?
1.11.1.13
Mn2+ + H+ + H2O2
unique binding and oxidation site for Mn2+, single Mn atom is hexacoordinate, with two water ligands and four carboxylate ligands from heme propionate 6 and amino acids Glu-35, Glu-39 and Asp-179
439854
Phanerochaete chrysosporium OGC101
Mn3+ + H2O
Mn3+ oxidizes phenolic lignin model compounds
439854
Phanerochaete chrysosporium OGC101
-
1.11.1.13
Mn2+ + H+ + H2O2
unique binding and oxidation site for Mn2+, single Mn atom is hexacoordinate, with two water ligands and four carboxylate ligands from heme propionate 6 and amino acids Glu-35, Glu-39 and Asp-179
439854
Phanerochaete chrysosporium OGC101
Mn3+ + H2O
Mn3+ oxidizes lignin
439854
Phanerochaete chrysosporium OGC101
-
1.11.1.13
Mn2+ + H+ + H2O2
unique binding and oxidation site for Mn2+, single Mn atom is hexacoordinate, with two water ligands and four carboxylate ligands from heme propionate 6 and amino acids Glu-35, Glu-39 and Asp-179
439854
Phanerochaete chrysosporium OGC101
Mn3+ + H2O
Mn3+ oxidizes a variety of phenols
439854
Phanerochaete chrysosporium OGC101
-
1.11.1.13
additional information
catalytic cycle with oxidized intermediates MnP compound I and II
439854
Phanerochaete chrysosporium
?
-
-
-
-
1.11.1.13
additional information
Mn2+-independent oxidation of small phenolic compounds, such as guaiacol and dimethoxyphenol, rates are greatly reduced compared with the Mn-mediated reaction
439854
Phanerochaete chrysosporium
?
-
-
-
-
1.11.1.13
additional information
catalytic cycle with oxidized intermediates MnP compound I and II
439854
Phanerochaete chrysosporium OGC101
?
-
-
-
-
1.11.1.13
additional information
Mn2+-independent oxidation of small phenolic compounds, such as guaiacol and dimethoxyphenol, rates are greatly reduced compared with the Mn-mediated reaction
439854
Phanerochaete chrysosporium OGC101
?
-
-
-
-
Temperature Stability [°C] (protein specific)
EC Number
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
1.11.1.13
55
-
pH 4.5, 1.4 min, 50% loss of activity, both Mn2+ and Cd2+ protect MnP from thermal denaturation more efficiently than Ca2+ at pH 4.5, extending the half-life more than 2fold at 1 mM, combination of 0.5 mM Mn2+ and 0.5 mM Cd2+ extends the half-life more than 10fold
Phanerochaete chrysosporium
Turnover Number [1/s] (protein specific)
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
1.11.1.13
additional information
-
additional information
-
Phanerochaete chrysosporium
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.11.1.13
4.5
-
-
Phanerochaete chrysosporium