EC Number | Application | Comment | Organism |
---|---|---|---|
1.11.1.13 | environmental protection | mediated system of degradation is potentially valuable for degradation of synthetic polymers and of environmental pollutants | Phanerodontia chrysosporium |
1.11.1.13 | paper production | mediated system of degradation is potentially valuable for pulp and paper industries | Phanerodontia chrysosporium |
EC Number | Cloned (Comment) | Organism |
---|---|---|
1.11.1.13 | expression of A48C/A63C double mutant mnp gene in Escherichia coli XL-1 Blue | Phanerodontia chrysosporium |
EC Number | Crystallization (Comment) | Organism |
---|---|---|
1.11.1.13 | crystal structure | Phanerodontia chrysosporium |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
1.11.1.13 | A48C/A63C | A48C and A63C double mutant with an engineered disulfide bond near the distal calcium binding site to restrict the movement of helix B upon loss of calcium and to stabilize against this loss, thermal and pH-stability is improved compared with that of native and recombinant MnP, thermally treated enzyme contains one calcium and retains a percentage of its activity | Phanerodontia chrysosporium |
EC Number | General Stability | Organism |
---|---|---|
1.11.1.13 | calcium stabilizes | Phanerodontia chrysosporium |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
1.11.1.13 | Ca2+ | MnP calcium binding site, calcium content: 4 mol per mol of native MnP, 2 mol per mol of recombinant and A48C/A63C double mutant MnP, calcium decreases the rate of thermal inactivation | Phanerodontia chrysosporium |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.11.1.13 | Phanerodontia chrysosporium | - |
- |
- |
EC Number | Posttranslational Modification | Comment | Organism |
---|---|---|---|
1.11.1.13 | glycoprotein | glycosylation stabilizes MnP | Phanerodontia chrysosporium |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.11.1.13 | isoenzyme H4 | Phanerodontia chrysosporium |
1.11.1.13 | purification of recombinant A48C/A63C double mutant MnP, expressed in Escherichia coli | Phanerodontia chrysosporium |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.11.1.13 | Mn2+ + H+ + H2O2 | - |
Phanerodontia chrysosporium | Mn3+ + H2O | - |
? | |
1.11.1.13 | additional information | structural properties | Phanerodontia chrysosporium | ? | - |
? |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.11.1.13 | additional information | - |
susceptible to thermal inactivation due to the loss of calcium, calcium content after thermal treatment at 37°C for 15 min: 1.5 mol per mol of native MnP, 1 mol per mol of recombinant MnP and 1.3 mol per mol of A48C/A63C double mutant MnP, biphasic inactivation kinetics, recombinant MnP is more sensitive than native MnP as a result of the lack of glycosylation, calcium decreases the rate of thermal inactivation and reactivates MnP up to 50% of its original activity, while EGTA increases the rate of inactivation | Phanerodontia chrysosporium |
1.11.1.13 | 37 | - |
recombinant MnP: 5 min, 50% loss of activity, A48C/A63C double mutant MnP: relatively stable | Phanerodontia chrysosporium |
1.11.1.13 | 52 | - |
recombinant MnP: 20 s, 50% loss of activity, A48C/A63C double mutant MnP: 2 min, 50% loss of activity | Phanerodontia chrysosporium |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.11.1.13 | 24 | - |
Mn2+ | A48C/A63C double mutant MnP after thermal treatment at 37°C for 15 min | Phanerodontia chrysosporium | |
1.11.1.13 | 218 | - |
Mn2+ | native MnP | Phanerodontia chrysosporium | |
1.11.1.13 | 270 | 273 | Mn2+ | recombinant MnP and A48C/A63C double mutant MnP | Phanerodontia chrysosporium |
EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|---|
1.11.1.13 | additional information | - |
recombinant MnP is more sensitive than native MnP as a result of lack of glycosylation | Phanerodontia chrysosporium |
1.11.1.13 | 4.5 | - |
recombinant MnP, native MnP and A48C/A63C double mutant MnP are stable | Phanerodontia chrysosporium |
1.11.1.13 | 6 | - |
native and A48C/A63C double mutant MnP: 1 h, less than 40% loss of activity | Phanerodontia chrysosporium |
1.11.1.13 | 7 | - |
native MnP: 1 h, 80% loss of activity, A48C/A63C double mutant MnP: 1 h, 60% loss of activity | Phanerodontia chrysosporium |
1.11.1.13 | 8 | - |
recombinant MnP: inactivation within 1 min, native MnP: inactivation within 15 min, A48C/A63C double mutant MnP: 1 h, 80% loss of activity | Phanerodontia chrysosporium |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.11.1.13 | heme | enzyme contains a pentacoordinated, essentially high-spin ferric heme | Phanerodontia chrysosporium | |
1.11.1.13 | heme | heme protein | Phanerodontia chrysosporium |