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Literature summary extracted from

  • Wariishi, H.; Valli, K.; Renganathan, V.; Gold, M.H.
    Thiol-mediated oxidation of nonphenolic lignin model compounds by manganese peroxidase of Phanerochaete chrysosporium (1989), J. Biol. Chem., 264, 14185-14191.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.11.1.13 diphosphate less activation than by lactate or malonate Phanerodontia chrysosporium
1.11.1.13 H2O2 H2O2-dependent Phanerodontia chrysosporium
1.11.1.13 Lactate activates, stabilizes Mn3+ in aqueous solution with a relatively high redox potential Phanerodontia chrysosporium
1.11.1.13 malonate stabilizes Mn3+ at a relatively high redox potential and facilitate oxidation of organic substrates Phanerodontia chrysosporium
1.11.1.13 oxalate activates by chelating and stabilizing Mn3+ Phanerodontia chrysosporium
1.11.1.13 oxalate less activation than by lactate or malonate Phanerodontia chrysosporium

Application

EC Number Application Comment Organism
1.11.1.13 environmental protection thiol-mediated degradation of dimeric model compounds and of polymeric lignin by MnP has potential applications in the degradation of industrial lignins Phanerodontia chrysosporium

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.11.1.13 cDNA sequence encoding a MnP isoenzyme is determined Phanerodontia chrysosporium

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.11.1.13 glutathione
-
 Phanerodontia chrysosporium

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.11.1.13 extracellular
-
 Phanerodontia chrysosporium
-
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.11.1.13 46000
-
-
 Phanerodontia chrysosporium

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.11.1.13 Mn2+ + H+ + H2O2 Phanerodontia chrysosporium important component of lignin degradation system Mn3+ + H2O Mn3+ is produced under lignolytic conditions ?
1.11.1.13 Mn2+ + H+ + H2O2 Phanerodontia chrysosporium OGC101 important component of lignin degradation system Mn3+ + H2O Mn3+ is produced under lignolytic conditions ?

Organism

EC Number Organism UniProt Comment Textmining
1.11.1.13 Phanerodontia chrysosporium
-
-
-
1.11.1.13 Phanerodontia chrysosporium
-
 white rot basidomycete
-
1.11.1.13 Phanerodontia chrysosporium OGC101
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
1.11.1.13 glycoprotein
-
 Phanerodontia chrysosporium

Purification (Commentary)

EC Number Purification (Comment) Organism
1.11.1.13 isoenzyme 1 Phanerodontia chrysosporium

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.11.1.13 Mn2+ + H+ + H2O2
-
 Phanerodontia chrysosporium Mn3+ + H2O Mn3+ oxidizes phenolic lignin model compounds ?
1.11.1.13 Mn2+ + H+ + H2O2
-
 Phanerodontia chrysosporium Mn3+ + H2O in presence of Mn2+, H2O2 and glutathione MnP oxidizes by Mn3+ nonphenolic beta-aryl ether lignin model compounds, veratryl alcohol, anisyl alcohol, benzyl alcohol and thiols to thiyl radicals which abstracts a hydrogen from the substrate forming a benzylic radical, mechanism, glutathione can be replaced by dithiothreitol, dithioerythritol or cysteine ?
1.11.1.13 Mn2+ + H+ + H2O2
-
 Phanerodontia chrysosporium Mn3+ + H2O Mn3+ acts as obligatory redox coupler, oxidizing various phenols, dyes and amines ?
1.11.1.13 Mn2+ + H+ + H2O2 important component of lignin degradation system Phanerodontia chrysosporium Mn3+ + H2O Mn3+ is produced under lignolytic conditions ?
1.11.1.13 Mn2+ + H+ + H2O2
-
 Phanerodontia chrysosporium OGC101 Mn3+ + H2O Mn3+ oxidizes phenolic lignin model compounds ?
1.11.1.13 Mn2+ + H+ + H2O2
-
 Phanerodontia chrysosporium OGC101 Mn3+ + H2O in presence of Mn2+, H2O2 and glutathione MnP oxidizes by Mn3+ nonphenolic beta-aryl ether lignin model compounds, veratryl alcohol, anisyl alcohol, benzyl alcohol and thiols to thiyl radicals which abstracts a hydrogen from the substrate forming a benzylic radical, mechanism, glutathione can be replaced by dithiothreitol, dithioerythritol or cysteine ?
1.11.1.13 Mn2+ + H+ + H2O2
-
 Phanerodontia chrysosporium OGC101 Mn3+ + H2O Mn3+ acts as obligatory redox coupler, oxidizing various phenols, dyes and amines ?
1.11.1.13 Mn2+ + H+ + H2O2 important component of lignin degradation system Phanerodontia chrysosporium OGC101 Mn3+ + H2O Mn3+ is produced under lignolytic conditions ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.11.1.13 37
-
 assay at Phanerodontia chrysosporium

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.11.1.13 additional information
-
 pI: 4.9 Phanerodontia chrysosporium

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.11.1.13 3.1 6.1 veratryl alcohol oxidation, activity increases with increasing pH Phanerodontia chrysosporium

Cofactor

EC Number Cofactor Comment Organism Structure
1.11.1.13 heme one iron protoporphyrin IX prosthetic group per enzyme molecule Phanerodontia chrysosporium
1.11.1.13 heme heme protein Phanerodontia chrysosporium