BRENDA - Enzyme Database

Thiol-mediated oxidation of nonphenolic lignin model compounds by manganese peroxidase of Phanerochaete chrysosporium

Wariishi, H.; Valli, K.; Renganathan, V.; Gold, M.H.; J. Biol. Chem. 264, 14185-14191 (1989)

Data extracted from this reference:

Activating Compound
EC Number
Activating Compound
Commentary
Organism
Structure
1.11.1.13
diphosphate
less activation than by lactate or malonate
Phanerochaete chrysosporium
1.11.1.13
H2O2
H2O2-dependent
Phanerochaete chrysosporium
1.11.1.13
Lactate
activates, stabilizes Mn3+ in aqueous solution with a relatively high redox potential
Phanerochaete chrysosporium
1.11.1.13
malonate
stabilizes Mn3+ at a relatively high redox potential and facilitate oxidation of organic substrates
Phanerochaete chrysosporium
1.11.1.13
oxalate
activates by chelating and stabilizing Mn3+; less activation than by lactate or malonate
Phanerochaete chrysosporium
Application
EC Number
Application
Commentary
Organism
1.11.1.13
environmental protection
thiol-mediated degradation of dimeric model compounds and of polymeric lignin by MnP has potential applications in the degradation of industrial lignins
Phanerochaete chrysosporium
Cloned(Commentary)
EC Number
Commentary
Organism
1.11.1.13
cDNA sequence encoding a MnP isoenzyme is determined
Phanerochaete chrysosporium
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
1.11.1.13
glutathione
-
Phanerochaete chrysosporium
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
1.11.1.13
extracellular
-
Phanerochaete chrysosporium
-
-
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.11.1.13
46000
-
-
Phanerochaete chrysosporium
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.11.1.13
Mn2+ + H+ + H2O2
Phanerochaete chrysosporium
important component of lignin degradation system
Mn3+ + H2O
Mn3+ is produced under lignolytic conditions
Phanerochaete chrysosporium
?
1.11.1.13
Mn2+ + H+ + H2O2
Phanerochaete chrysosporium OGC101
important component of lignin degradation system
Mn3+ + H2O
Mn3+ is produced under lignolytic conditions
Phanerochaete chrysosporium OGC101
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
1.11.1.13
Phanerochaete chrysosporium
-
; white rot basidomycete
-
1.11.1.13
Phanerochaete chrysosporium OGC101
-
-
-
Posttranslational Modification
EC Number
Posttranslational Modification
Commentary
Organism
1.11.1.13
glycoprotein
-
Phanerochaete chrysosporium
Purification (Commentary)
EC Number
Commentary
Organism
1.11.1.13
isoenzyme 1
Phanerochaete chrysosporium
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.11.1.13
Mn2+ + H+ + H2O2
-
439831
Phanerochaete chrysosporium
Mn3+ + H2O
Mn3+ oxidizes phenolic lignin model compounds
439831
Phanerochaete chrysosporium
-
1.11.1.13
Mn2+ + H+ + H2O2
-
439831
Phanerochaete chrysosporium
Mn3+ + H2O
in presence of Mn2+, H2O2 and glutathione MnP oxidizes by Mn3+ nonphenolic beta-aryl ether lignin model compounds, veratryl alcohol, anisyl alcohol, benzyl alcohol and thiols to thiyl radicals which abstracts a hydrogen from the substrate forming a benzylic radical, mechanism, glutathione can be replaced by dithiothreitol, dithioerythritol or cysteine
439831
Phanerochaete chrysosporium
-
1.11.1.13
Mn2+ + H+ + H2O2
-
439831
Phanerochaete chrysosporium
Mn3+ + H2O
Mn3+ acts as obligatory redox coupler, oxidizing various phenols, dyes and amines
439831
Phanerochaete chrysosporium
-
1.11.1.13
Mn2+ + H+ + H2O2
important component of lignin degradation system
439831
Phanerochaete chrysosporium
Mn3+ + H2O
Mn3+ is produced under lignolytic conditions
439831
Phanerochaete chrysosporium
?
1.11.1.13
Mn2+ + H+ + H2O2
-
439831
Phanerochaete chrysosporium OGC101
Mn3+ + H2O
Mn3+ oxidizes phenolic lignin model compounds
439831
Phanerochaete chrysosporium OGC101
-
1.11.1.13
Mn2+ + H+ + H2O2
-
439831
Phanerochaete chrysosporium OGC101
Mn3+ + H2O
in presence of Mn2+, H2O2 and glutathione MnP oxidizes by Mn3+ nonphenolic beta-aryl ether lignin model compounds, veratryl alcohol, anisyl alcohol, benzyl alcohol and thiols to thiyl radicals which abstracts a hydrogen from the substrate forming a benzylic radical, mechanism, glutathione can be replaced by dithiothreitol, dithioerythritol or cysteine
439831
Phanerochaete chrysosporium OGC101
-
1.11.1.13
Mn2+ + H+ + H2O2
-
439831
Phanerochaete chrysosporium OGC101
Mn3+ + H2O
Mn3+ acts as obligatory redox coupler, oxidizing various phenols, dyes and amines
439831
Phanerochaete chrysosporium OGC101
-
1.11.1.13
Mn2+ + H+ + H2O2
important component of lignin degradation system
439831
Phanerochaete chrysosporium OGC101
Mn3+ + H2O
Mn3+ is produced under lignolytic conditions
439831
Phanerochaete chrysosporium OGC101
?
Temperature Optimum [°C]
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
1.11.1.13
37
-
assay at
Phanerochaete chrysosporium
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.11.1.13
additional information
-
pI: 4.9
Phanerochaete chrysosporium
pH Range
EC Number
pH Minimum
pH Maximum
Commentary
Organism
1.11.1.13
3.1
6.1
veratryl alcohol oxidation, activity increases with increasing pH
Phanerochaete chrysosporium
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
1.11.1.13
heme
heme protein; one iron protoporphyrin IX prosthetic group per enzyme molecule
Phanerochaete chrysosporium
Activating Compound (protein specific)
EC Number
Activating Compound
Commentary
Organism
Structure
1.11.1.13
diphosphate
less activation than by lactate or malonate
Phanerochaete chrysosporium
1.11.1.13
H2O2
H2O2-dependent
Phanerochaete chrysosporium
1.11.1.13
Lactate
activates, stabilizes Mn3+ in aqueous solution with a relatively high redox potential
Phanerochaete chrysosporium
1.11.1.13
malonate
stabilizes Mn3+ at a relatively high redox potential and facilitate oxidation of organic substrates
Phanerochaete chrysosporium
1.11.1.13
oxalate
activates by chelating and stabilizing Mn3+; less activation than by lactate or malonate
Phanerochaete chrysosporium
Application (protein specific)
EC Number
Application
Commentary
Organism
1.11.1.13
environmental protection
thiol-mediated degradation of dimeric model compounds and of polymeric lignin by MnP has potential applications in the degradation of industrial lignins
Phanerochaete chrysosporium
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
1.11.1.13
cDNA sequence encoding a MnP isoenzyme is determined
Phanerochaete chrysosporium
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
1.11.1.13
heme
heme protein; one iron protoporphyrin IX prosthetic group per enzyme molecule
Phanerochaete chrysosporium
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
1.11.1.13
glutathione
-
Phanerochaete chrysosporium
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
1.11.1.13
extracellular
-
Phanerochaete chrysosporium
-
-
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.11.1.13
46000
-
-
Phanerochaete chrysosporium
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.11.1.13
Mn2+ + H+ + H2O2
Phanerochaete chrysosporium
important component of lignin degradation system
Mn3+ + H2O
Mn3+ is produced under lignolytic conditions
Phanerochaete chrysosporium
?
1.11.1.13
Mn2+ + H+ + H2O2
Phanerochaete chrysosporium OGC101
important component of lignin degradation system
Mn3+ + H2O
Mn3+ is produced under lignolytic conditions
Phanerochaete chrysosporium OGC101
?
Posttranslational Modification (protein specific)
EC Number
Posttranslational Modification
Commentary
Organism
1.11.1.13
glycoprotein
-
Phanerochaete chrysosporium
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
1.11.1.13
isoenzyme 1
Phanerochaete chrysosporium
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.11.1.13
Mn2+ + H+ + H2O2
-
439831
Phanerochaete chrysosporium
Mn3+ + H2O
Mn3+ oxidizes phenolic lignin model compounds
439831
Phanerochaete chrysosporium
-
1.11.1.13
Mn2+ + H+ + H2O2
-
439831
Phanerochaete chrysosporium
Mn3+ + H2O
in presence of Mn2+, H2O2 and glutathione MnP oxidizes by Mn3+ nonphenolic beta-aryl ether lignin model compounds, veratryl alcohol, anisyl alcohol, benzyl alcohol and thiols to thiyl radicals which abstracts a hydrogen from the substrate forming a benzylic radical, mechanism, glutathione can be replaced by dithiothreitol, dithioerythritol or cysteine
439831
Phanerochaete chrysosporium
-
1.11.1.13
Mn2+ + H+ + H2O2
-
439831
Phanerochaete chrysosporium
Mn3+ + H2O
Mn3+ acts as obligatory redox coupler, oxidizing various phenols, dyes and amines
439831
Phanerochaete chrysosporium
-
1.11.1.13
Mn2+ + H+ + H2O2
important component of lignin degradation system
439831
Phanerochaete chrysosporium
Mn3+ + H2O
Mn3+ is produced under lignolytic conditions
439831
Phanerochaete chrysosporium
?
1.11.1.13
Mn2+ + H+ + H2O2
-
439831
Phanerochaete chrysosporium OGC101
Mn3+ + H2O
Mn3+ oxidizes phenolic lignin model compounds
439831
Phanerochaete chrysosporium OGC101
-
1.11.1.13
Mn2+ + H+ + H2O2
-
439831
Phanerochaete chrysosporium OGC101
Mn3+ + H2O
in presence of Mn2+, H2O2 and glutathione MnP oxidizes by Mn3+ nonphenolic beta-aryl ether lignin model compounds, veratryl alcohol, anisyl alcohol, benzyl alcohol and thiols to thiyl radicals which abstracts a hydrogen from the substrate forming a benzylic radical, mechanism, glutathione can be replaced by dithiothreitol, dithioerythritol or cysteine
439831
Phanerochaete chrysosporium OGC101
-
1.11.1.13
Mn2+ + H+ + H2O2
-
439831
Phanerochaete chrysosporium OGC101
Mn3+ + H2O
Mn3+ acts as obligatory redox coupler, oxidizing various phenols, dyes and amines
439831
Phanerochaete chrysosporium OGC101
-
1.11.1.13
Mn2+ + H+ + H2O2
important component of lignin degradation system
439831
Phanerochaete chrysosporium OGC101
Mn3+ + H2O
Mn3+ is produced under lignolytic conditions
439831
Phanerochaete chrysosporium OGC101
?
Temperature Optimum [°C] (protein specific)
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
1.11.1.13
37
-
assay at
Phanerochaete chrysosporium
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.11.1.13
additional information
-
pI: 4.9
Phanerochaete chrysosporium
pH Range (protein specific)
EC Number
pH Minimum
pH Maximum
Commentary
Organism
1.11.1.13
3.1
6.1
veratryl alcohol oxidation, activity increases with increasing pH
Phanerochaete chrysosporium