BRENDA - Enzyme Database

Characteristics and N-terminal amino acid sequence of a manganese peroxidase purified from Lentinula edodes cultures grown on a commercial wood substrate

Forrester, I.T.; Grabski, A.C.; Mishra, C.; Kelley, B.D.; Strickland, W.N.; Leatham, G.F.; Burgess, R.R.; Appl. Microbiol. Biotechnol. 33, 359-365 (1990)

Data extracted from this reference:

Activating Compound
EC Number
Activating Compound
Commentary
Organism
Structure
1.11.1.13
diphosphate
stimulates by chelating and stabilizing Mn3+
Lentinula edodes
1.11.1.13
dithiothreitol
greatly enhances the oxidation of verartryl alcohol, lignin-model compounds and lignin
Lentinula edodes
1.11.1.13
glutathione
greatly enhances the oxidation of verartryl alcohol, lignin-model compounds and lignin
Lentinula edodes
1.11.1.13
H2O2
H2O2-dependent
Lentinula edodes
1.11.1.13
L-malate
activates by chelating and stabilizing Mn3+
Lentinula edodes
1.11.1.13
L-Tartrate
stimulates by chelating and stabilizing Mn3+
Lentinula edodes
1.11.1.13
malonate
stimulates by chelating and stabilizing Mn3+
Lentinula edodes
1.11.1.13
methylmalonate
stimulates by chelating and stabilizing Mn3+
Lentinula edodes
1.11.1.13
additional information
no Mn3+ chelators and activators: acetate, propionate, citrate, D-malate, ethylene glycol; succinate is no Mn3+ chelator and activator
Lentinula edodes
1.11.1.13
oxalate
activates by chelating and stabilizing Mn3+
Lentinula edodes
1.11.1.13
oxaloacetate
stimulates by chelating and stabilizing Mn3+
Lentinula edodes
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
1.11.1.13
additional information
not inhibited by 0.1 mM Cu2+, Co2+ or salicylic acid; not inhibited by salicylic acid
Lentinula edodes
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
1.11.1.13
extracellular
-
Lentinula edodes
-
-
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.11.1.13
44600
-
x * 44600, SDS-PAGE
Lentinula edodes
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
1.11.1.13
Lentinula edodes
-
syn. Lentinus edodes, heterodikaryon strain 861; white rot basidomycete, produces edible shiitake mushroom
-
Purification (Commentary)
EC Number
Commentary
Organism
1.11.1.13
MnP1: 19fold purification
Lentinula edodes
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [mol/min/mg]
Specific Activity Maximum [mol/min/mg]
Commentary
Organism
1.11.1.13
660
-
o-toluidine as substrate for Mn3+-oxidation
Lentinula edodes
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.11.1.13
Mn2+ + H+ + H2O2
-
439830
Lentinula edodes
Mn3+ + H2O
chelating organic acids facilitate the dissociation of Mn3+ from enzyme
439830
Lentinula edodes
?
1.11.1.13
Mn2+ + H+ + H2O2
-
439830
Lentinula edodes
Mn3+ + H2O
Mn3+ oxidizes phenolic lignin model compounds
439830
Lentinula edodes
?
1.11.1.13
Mn2+ + H+ + H2O2
-
439830
Lentinula edodes
Mn3+ + H2O
Mn3+ oxidizes vanillylacetone
439830
Lentinula edodes
?
1.11.1.13
Mn2+ + H+ + H2O2
-
439830
Lentinula edodes
Mn3+ + H2O
chelation of Mn3+ by organic acids stabilizes Mn3+ at a high redox potential
439830
Lentinula edodes
?
1.11.1.13
additional information
enzyme is able to oxidatively depolymerize both dimeric lignin-model compounds and milled spruce-wood lignin
439830
Lentinula edodes
?
-
-
-
-
1.11.1.13
additional information
enzyme oxidizes 2,2-azino-di-3-ethylbenzothiazoline-6-sulfonate
439830
Lentinula edodes
?
-
-
-
-
1.11.1.13
additional information
enzyme oxidizes veratryl alcohol and o-tolidine
439830
Lentinula edodes
?
-
-
-
-
1.11.1.13
additional information
in presence of H2O2 and Mn2+ the enzyme oxidizes lignin and lignin-model compounds
439830
Lentinula edodes
?
-
-
-
-
Subunits
EC Number
Subunits
Commentary
Organism
1.11.1.13
?
x * 44600, SDS-PAGE
Lentinula edodes
Temperature Optimum [C]
EC Number
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
1.11.1.13
40
-
assay at
Lentinula edodes
Temperature Range [C]
EC Number
Temperature Minimum [C]
Temperature Maximum [C]
Commentary
Organism
1.11.1.13
20
65
progressive enhancement of activity from 20C to 65C
Lentinula edodes
Temperature Stability [C]
EC Number
Temperature Stability Minimum [C]
Temperature Stability Maximum [C]
Commentary
Organism
1.11.1.13
100
-
complete inactivation after boiling for 5 min
Lentinula edodes
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.11.1.13
additional information
-
pI: 3.2
Lentinula edodes
1.11.1.13
3.5
5.5
-
Lentinula edodes
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
1.11.1.13
heme
heme-iron protein; one iron protoporphyrin IX prosthetic group per enzyme molecule
Lentinula edodes
Activating Compound (protein specific)
EC Number
Activating Compound
Commentary
Organism
Structure
1.11.1.13
diphosphate
stimulates by chelating and stabilizing Mn3+
Lentinula edodes
1.11.1.13
dithiothreitol
greatly enhances the oxidation of verartryl alcohol, lignin-model compounds and lignin
Lentinula edodes
1.11.1.13
glutathione
greatly enhances the oxidation of verartryl alcohol, lignin-model compounds and lignin
Lentinula edodes
1.11.1.13
H2O2
H2O2-dependent
Lentinula edodes
1.11.1.13
L-malate
activates by chelating and stabilizing Mn3+
Lentinula edodes
1.11.1.13
L-Tartrate
stimulates by chelating and stabilizing Mn3+
Lentinula edodes
1.11.1.13
malonate
stimulates by chelating and stabilizing Mn3+
Lentinula edodes
1.11.1.13
methylmalonate
stimulates by chelating and stabilizing Mn3+
Lentinula edodes
1.11.1.13
additional information
no Mn3+ chelators and activators: acetate, propionate, citrate, D-malate, ethylene glycol; succinate is no Mn3+ chelator and activator
Lentinula edodes
1.11.1.13
oxalate
activates by chelating and stabilizing Mn3+
Lentinula edodes
1.11.1.13
oxaloacetate
stimulates by chelating and stabilizing Mn3+
Lentinula edodes
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
1.11.1.13
heme
heme-iron protein; one iron protoporphyrin IX prosthetic group per enzyme molecule
Lentinula edodes
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
1.11.1.13
additional information
not inhibited by 0.1 mM Cu2+, Co2+ or salicylic acid; not inhibited by salicylic acid
Lentinula edodes
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
1.11.1.13
extracellular
-
Lentinula edodes
-
-
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.11.1.13
44600
-
x * 44600, SDS-PAGE
Lentinula edodes
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
1.11.1.13
MnP1: 19fold purification
Lentinula edodes
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [mol/min/mg]
Specific Activity Maximum [mol/min/mg]
Commentary
Organism
1.11.1.13
660
-
o-toluidine as substrate for Mn3+-oxidation
Lentinula edodes
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.11.1.13
Mn2+ + H+ + H2O2
-
439830
Lentinula edodes
Mn3+ + H2O
chelating organic acids facilitate the dissociation of Mn3+ from enzyme
439830
Lentinula edodes
?
1.11.1.13
Mn2+ + H+ + H2O2
-
439830
Lentinula edodes
Mn3+ + H2O
Mn3+ oxidizes phenolic lignin model compounds
439830
Lentinula edodes
?
1.11.1.13
Mn2+ + H+ + H2O2
-
439830
Lentinula edodes
Mn3+ + H2O
Mn3+ oxidizes vanillylacetone
439830
Lentinula edodes
?
1.11.1.13
Mn2+ + H+ + H2O2
-
439830
Lentinula edodes
Mn3+ + H2O
chelation of Mn3+ by organic acids stabilizes Mn3+ at a high redox potential
439830
Lentinula edodes
?
1.11.1.13
additional information
enzyme is able to oxidatively depolymerize both dimeric lignin-model compounds and milled spruce-wood lignin
439830
Lentinula edodes
?
-
-
-
-
1.11.1.13
additional information
enzyme oxidizes 2,2-azino-di-3-ethylbenzothiazoline-6-sulfonate
439830
Lentinula edodes
?
-
-
-
-
1.11.1.13
additional information
enzyme oxidizes veratryl alcohol and o-tolidine
439830
Lentinula edodes
?
-
-
-
-
1.11.1.13
additional information
in presence of H2O2 and Mn2+ the enzyme oxidizes lignin and lignin-model compounds
439830
Lentinula edodes
?
-
-
-
-
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
1.11.1.13
?
x * 44600, SDS-PAGE
Lentinula edodes
Temperature Optimum [C] (protein specific)
EC Number
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
1.11.1.13
40
-
assay at
Lentinula edodes
Temperature Range [C] (protein specific)
EC Number
Temperature Minimum [C]
Temperature Maximum [C]
Commentary
Organism
1.11.1.13
20
65
progressive enhancement of activity from 20C to 65C
Lentinula edodes
Temperature Stability [C] (protein specific)
EC Number
Temperature Stability Minimum [C]
Temperature Stability Maximum [C]
Commentary
Organism
1.11.1.13
100
-
complete inactivation after boiling for 5 min
Lentinula edodes
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.11.1.13
additional information
-
pI: 3.2
Lentinula edodes
1.11.1.13
3.5
5.5
-
Lentinula edodes