BRENDA - Enzyme Database

Manganese(II) oxidation by manganese peroxidase from the basidiomycete Phanerochaete chrysosporium. Kinetic mechanism and role of chelators

Wariishi, H.; Valli, K.; Gold, M.H.; J. Biol. Chem. 267, 23688-23695 (1992)

Data extracted from this reference:

Activating Compound
EC Number
Activating Compound
Commentary
Organism
Structure
1.11.1.13
1,10-phenanthroline
slightly activates, chelates Mn3+
Phanerochaete chrysosporium
1.11.1.13
alpha-hydroxy acid
-
Phanerochaete chrysosporium
1.11.1.13
cellobionate
activates, chelates Mn3+
Phanerochaete chrysosporium
1.11.1.13
citrate
activates, stabilizes Mn3+ in aqueous solution with a relatively high redox potential
Phanerochaete chrysosporium
1.11.1.13
H2O2
H2O2-dependent
Phanerochaete chrysosporium
1.11.1.13
L-malate
activates by chelating and stabilizing Mn3+
Phanerochaete chrysosporium
1.11.1.13
L-Tartrate
activates, stabilizes Mn3+ in aqueous solution with a relatively high redox potential
Phanerochaete chrysosporium
1.11.1.13
Lactate
activates, stabilizes Mn3+ in aqueous solution with a relatively high redox potential
Phanerochaete chrysosporium
1.11.1.13
malonate
activates, stabilizes Mn3+ in aqueous solution with a relatively high redox potential, most effective physiological chelator excreted by the fungus
Phanerochaete chrysosporium
1.11.1.13
additional information
dramatic stimulation by chelating organic acids as C2- and C3-dicarboxylic or alpha-hydroxyl acids facilitate the dissociation of Mn(III) from manganese-enzyme complex, greater activation with weakly binding chelators with a low binding constant, e.g. lactate or tartrate; succinate is no Mn3+ chelator and activator
Phanerochaete chrysosporium
1.11.1.13
nitrilotriacetate
slightly activates, chelates Mn3+
Phanerochaete chrysosporium
1.11.1.13
oxalate
activates by chelating and stabilizing Mn3+
Phanerochaete chrysosporium
1.11.1.13
Polyglutamate
slightly activates, stabilizes Mn3+ in aqueous solution with a relatively high redox potential
Phanerochaete chrysosporium
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
1.11.1.13
1,10-phenanthroline
inhibits competitive Mn(III)-malonate formation
Phanerochaete chrysosporium
1.11.1.13
cellobionate
inhibits competitive Mn(III)-malonate formation
Phanerochaete chrysosporium
1.11.1.13
additional information
not inhibited by succinate
Phanerochaete chrysosporium
1.11.1.13
nitrilotriacetate
inhibits competitive Mn(III)-malonate formation
Phanerochaete chrysosporium
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
1.11.1.13
extracellular
-
Phanerochaete chrysosporium
-
-
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.11.1.13
46000
-
-
Phanerochaete chrysosporium
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.11.1.13
Mn2+ + H+ + H2O2
Phanerochaete chrysosporium
important component of lignin degradation system
Mn3+ + H2O
Mn3+ is stabilized by chelating agents, malonate is the most effective physiological chelator excreted by the fungus
Phanerochaete chrysosporium
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
1.11.1.13
Phanerochaete chrysosporium
-
; MnP exists as several closely related isoenzymes; white rot basidomycete
-
1.11.1.13
Phanerochaete chrysosporium OGC101
-
-
-
Posttranslational Modification
EC Number
Posttranslational Modification
Commentary
Organism
1.11.1.13
glycoprotein
-
Phanerochaete chrysosporium
Purification (Commentary)
EC Number
Commentary
Organism
1.11.1.13
isoenzyme 1
Phanerochaete chrysosporium
Reaction
EC Number
Reaction
Commentary
Organism
1.11.1.13
2 Mn(II) + 2 H+ + H2O2 = 2 Mn(III) + 2 H2O
ping-pong mechanism
Phanerochaete chrysosporium
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.11.1.13
Mn2+ + H+ + H2O2
each catalytic cycle step is irreversible
439821
Phanerochaete chrysosporium
Mn3+ + H2O
alpha-hydroxy acids, e.g. lactate, facilitate the dissociation of Mn3+ from enzyme
439821
Phanerochaete chrysosporium
ir
1.11.1.13
Mn2+ + H+ + H2O2
each catalytic cycle step is irreversible
439821
Phanerochaete chrysosporium
Mn3+ + H2O
Mn3+ oxidizes phenolic lignin model compounds
439821
Phanerochaete chrysosporium
ir
1.11.1.13
Mn2+ + H+ + H2O2
each catalytic cycle step is irreversible
439821
Phanerochaete chrysosporium
Mn3+ + H2O
Mn3+ oxidizes vanillyl alcohol
439821
Phanerochaete chrysosporium
ir
1.11.1.13
Mn2+ + H+ + H2O2
each catalytic cycle step is irreversible
439821
Phanerochaete chrysosporium
Mn3+ + H2O
Mn3+ oxidizes lignin
439821
Phanerochaete chrysosporium
ir
1.11.1.13
Mn2+ + H+ + H2O2
each catalytic cycle step is irreversible
439821
Phanerochaete chrysosporium
Mn3+ + H2O
Mn3+-organic acid complexes oxidize terminal phenolic substrates in a second-order reaction
439821
Phanerochaete chrysosporium
ir
1.11.1.13
Mn2+ + H+ + H2O2
each catalytic cycle step is irreversible
439821
Phanerochaete chrysosporium
Mn3+ + H2O
Mn3+ oxidizes thiols
439821
Phanerochaete chrysosporium
ir
1.11.1.13
Mn2+ + H+ + H2O2
each catalytic cycle step is irreversible
439821
Phanerochaete chrysosporium
Mn3+ + H2O
Mn3+ acts as obligatory redox coupler, oxidizing various phenols, dyes and amines
439821
Phanerochaete chrysosporium
ir
1.11.1.13
Mn2+ + H+ + H2O2
each catalytic cycle step is irreversible
439821
Phanerochaete chrysosporium
Mn3+ + H2O
the diffusible product is Mn3+
439821
Phanerochaete chrysosporium
ir
1.11.1.13
Mn2+ + H+ + H2O2
each catalytic cycle step is irreversible
439821
Phanerochaete chrysosporium
Mn3+ + H2O
Mn3+ oxidizes amines
439821
Phanerochaete chrysosporium
ir
1.11.1.13
Mn2+ + H+ + H2O2
each catalytic cycle step is irreversible
439821
Phanerochaete chrysosporium
Mn3+ + H2O
chelation of Mn3+ by organic acids stabilizes Mn3+ at a high redox potential
439821
Phanerochaete chrysosporium
ir
1.11.1.13
Mn2+ + H+ + H2O2
free divalent Mn is the substrate, not Mn2+-complexes
439821
Phanerochaete chrysosporium
Mn3+ + H2O
alpha-hydroxy acids, e.g. lactate, facilitate the dissociation of Mn3+ from enzyme
439821
Phanerochaete chrysosporium
ir
1.11.1.13
Mn2+ + H+ + H2O2
free divalent Mn is the substrate, not Mn2+-complexes
439821
Phanerochaete chrysosporium
Mn3+ + H2O
Mn3+ oxidizes phenolic lignin model compounds
439821
Phanerochaete chrysosporium
ir
1.11.1.13
Mn2+ + H+ + H2O2
free divalent Mn is the substrate, not Mn2+-complexes
439821
Phanerochaete chrysosporium
Mn3+ + H2O
Mn3+ oxidizes vanillyl alcohol
439821
Phanerochaete chrysosporium
ir
1.11.1.13
Mn2+ + H+ + H2O2
free divalent Mn is the substrate, not Mn2+-complexes
439821
Phanerochaete chrysosporium
Mn3+ + H2O
Mn3+ oxidizes lignin
439821
Phanerochaete chrysosporium
ir
1.11.1.13
Mn2+ + H+ + H2O2
free divalent Mn is the substrate, not Mn2+-complexes
439821
Phanerochaete chrysosporium
Mn3+ + H2O
Mn3+-organic acid complexes oxidize terminal phenolic substrates in a second-order reaction
439821
Phanerochaete chrysosporium
ir
1.11.1.13
Mn2+ + H+ + H2O2
free divalent Mn is the substrate, not Mn2+-complexes
439821
Phanerochaete chrysosporium
Mn3+ + H2O
Mn3+ oxidizes thiols
439821
Phanerochaete chrysosporium
ir
1.11.1.13
Mn2+ + H+ + H2O2
free divalent Mn is the substrate, not Mn2+-complexes
439821
Phanerochaete chrysosporium
Mn3+ + H2O
Mn3+ acts as obligatory redox coupler, oxidizing various phenols, dyes and amines
439821
Phanerochaete chrysosporium
ir
1.11.1.13
Mn2+ + H+ + H2O2
free divalent Mn is the substrate, not Mn2+-complexes
439821
Phanerochaete chrysosporium
Mn3+ + H2O
the diffusible product is Mn3+
439821
Phanerochaete chrysosporium
ir
1.11.1.13
Mn2+ + H+ + H2O2
free divalent Mn is the substrate, not Mn2+-complexes
439821
Phanerochaete chrysosporium
Mn3+ + H2O
Mn3+ oxidizes amines
439821
Phanerochaete chrysosporium
ir
1.11.1.13
Mn2+ + H+ + H2O2
free divalent Mn is the substrate, not Mn2+-complexes
439821
Phanerochaete chrysosporium
Mn3+ + H2O
chelation of Mn3+ by organic acids stabilizes Mn3+ at a high redox potential
439821
Phanerochaete chrysosporium
ir
1.11.1.13
Mn2+ + H+ + H2O2
important component of lignin degradation system
439821
Phanerochaete chrysosporium
Mn3+ + H2O
Mn3+ is stabilized by chelating agents, malonate is the most effective physiological chelator excreted by the fungus
439821
Phanerochaete chrysosporium
?
1.11.1.13
Mn2+ + H+ + H2O2
each catalytic cycle step is irreversible
439821
Phanerochaete chrysosporium OGC101
Mn3+ + H2O
alpha-hydroxy acids, e.g. lactate, facilitate the dissociation of Mn3+ from enzyme
439821
Phanerochaete chrysosporium OGC101
ir
1.11.1.13
Mn2+ + H+ + H2O2
each catalytic cycle step is irreversible
439821
Phanerochaete chrysosporium OGC101
Mn3+ + H2O
Mn3+ oxidizes phenolic lignin model compounds
439821
Phanerochaete chrysosporium OGC101
ir
1.11.1.13
additional information
catalytic cycle with oxidized intermediates MnP compound I and II
439821
Phanerochaete chrysosporium
?
-
-
-
-
1.11.1.13
additional information
large substrates have no ready access to the catalytic center
439821
Phanerochaete chrysosporium
?
-
-
-
-
1.11.1.13
additional information
enzyme oxidizes 2,6-dimethoxyphenol
439821
Phanerochaete chrysosporium
?
-
-
-
-
1.11.1.13
additional information
catalytic cycle with oxidized intermediates MnP compound I and II
439821
Phanerochaete chrysosporium OGC101
?
-
-
-
-
1.11.1.13
additional information
large substrates have no ready access to the catalytic center
439821
Phanerochaete chrysosporium OGC101
?
-
-
-
-
1.11.1.13
additional information
enzyme oxidizes 2,6-dimethoxyphenol
439821
Phanerochaete chrysosporium OGC101
?
-
-
-
-
Temperature Optimum [C]
EC Number
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
1.11.1.13
25
-
assay at
Phanerochaete chrysosporium
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
1.11.1.13
heme
enzyme has a single manganese binding site near the heme; one iron protoporphyrin IX prosthetic group per enzyme molecule
Phanerochaete chrysosporium
Activating Compound (protein specific)
EC Number
Activating Compound
Commentary
Organism
Structure
1.11.1.13
1,10-phenanthroline
slightly activates, chelates Mn3+
Phanerochaete chrysosporium
1.11.1.13
alpha-hydroxy acid
-
Phanerochaete chrysosporium
1.11.1.13
cellobionate
activates, chelates Mn3+
Phanerochaete chrysosporium
1.11.1.13
citrate
activates, stabilizes Mn3+ in aqueous solution with a relatively high redox potential
Phanerochaete chrysosporium
1.11.1.13
H2O2
H2O2-dependent
Phanerochaete chrysosporium
1.11.1.13
L-malate
activates by chelating and stabilizing Mn3+
Phanerochaete chrysosporium
1.11.1.13
L-Tartrate
activates, stabilizes Mn3+ in aqueous solution with a relatively high redox potential
Phanerochaete chrysosporium
1.11.1.13
Lactate
activates, stabilizes Mn3+ in aqueous solution with a relatively high redox potential
Phanerochaete chrysosporium
1.11.1.13
malonate
activates, stabilizes Mn3+ in aqueous solution with a relatively high redox potential, most effective physiological chelator excreted by the fungus
Phanerochaete chrysosporium
1.11.1.13
additional information
dramatic stimulation by chelating organic acids as C2- and C3-dicarboxylic or alpha-hydroxyl acids facilitate the dissociation of Mn(III) from manganese-enzyme complex, greater activation with weakly binding chelators with a low binding constant, e.g. lactate or tartrate; succinate is no Mn3+ chelator and activator
Phanerochaete chrysosporium
1.11.1.13
nitrilotriacetate
slightly activates, chelates Mn3+
Phanerochaete chrysosporium
1.11.1.13
oxalate
activates by chelating and stabilizing Mn3+
Phanerochaete chrysosporium
1.11.1.13
Polyglutamate
slightly activates, stabilizes Mn3+ in aqueous solution with a relatively high redox potential
Phanerochaete chrysosporium
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
1.11.1.13
heme
enzyme has a single manganese binding site near the heme; one iron protoporphyrin IX prosthetic group per enzyme molecule
Phanerochaete chrysosporium
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
1.11.1.13
1,10-phenanthroline
inhibits competitive Mn(III)-malonate formation
Phanerochaete chrysosporium
1.11.1.13
cellobionate
inhibits competitive Mn(III)-malonate formation
Phanerochaete chrysosporium
1.11.1.13
additional information
not inhibited by succinate
Phanerochaete chrysosporium
1.11.1.13
nitrilotriacetate
inhibits competitive Mn(III)-malonate formation
Phanerochaete chrysosporium
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
1.11.1.13
extracellular
-
Phanerochaete chrysosporium
-
-
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.11.1.13
46000
-
-
Phanerochaete chrysosporium
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.11.1.13
Mn2+ + H+ + H2O2
Phanerochaete chrysosporium
important component of lignin degradation system
Mn3+ + H2O
Mn3+ is stabilized by chelating agents, malonate is the most effective physiological chelator excreted by the fungus
Phanerochaete chrysosporium
?
Posttranslational Modification (protein specific)
EC Number
Posttranslational Modification
Commentary
Organism
1.11.1.13
glycoprotein
-
Phanerochaete chrysosporium
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
1.11.1.13
isoenzyme 1
Phanerochaete chrysosporium
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.11.1.13
Mn2+ + H+ + H2O2
each catalytic cycle step is irreversible
439821
Phanerochaete chrysosporium
Mn3+ + H2O
alpha-hydroxy acids, e.g. lactate, facilitate the dissociation of Mn3+ from enzyme
439821
Phanerochaete chrysosporium
ir
1.11.1.13
Mn2+ + H+ + H2O2
each catalytic cycle step is irreversible
439821
Phanerochaete chrysosporium
Mn3+ + H2O
Mn3+ oxidizes phenolic lignin model compounds
439821
Phanerochaete chrysosporium
ir
1.11.1.13
Mn2+ + H+ + H2O2
each catalytic cycle step is irreversible
439821
Phanerochaete chrysosporium
Mn3+ + H2O
Mn3+ oxidizes vanillyl alcohol
439821
Phanerochaete chrysosporium
ir
1.11.1.13
Mn2+ + H+ + H2O2
each catalytic cycle step is irreversible
439821
Phanerochaete chrysosporium
Mn3+ + H2O
Mn3+ oxidizes lignin
439821
Phanerochaete chrysosporium
ir
1.11.1.13
Mn2+ + H+ + H2O2
each catalytic cycle step is irreversible
439821
Phanerochaete chrysosporium
Mn3+ + H2O
Mn3+-organic acid complexes oxidize terminal phenolic substrates in a second-order reaction
439821
Phanerochaete chrysosporium
ir
1.11.1.13
Mn2+ + H+ + H2O2
each catalytic cycle step is irreversible
439821
Phanerochaete chrysosporium
Mn3+ + H2O
Mn3+ oxidizes thiols
439821
Phanerochaete chrysosporium
ir
1.11.1.13
Mn2+ + H+ + H2O2
each catalytic cycle step is irreversible
439821
Phanerochaete chrysosporium
Mn3+ + H2O
Mn3+ acts as obligatory redox coupler, oxidizing various phenols, dyes and amines
439821
Phanerochaete chrysosporium
ir
1.11.1.13
Mn2+ + H+ + H2O2
each catalytic cycle step is irreversible
439821
Phanerochaete chrysosporium
Mn3+ + H2O
the diffusible product is Mn3+
439821
Phanerochaete chrysosporium
ir
1.11.1.13
Mn2+ + H+ + H2O2
each catalytic cycle step is irreversible
439821
Phanerochaete chrysosporium
Mn3+ + H2O
Mn3+ oxidizes amines
439821
Phanerochaete chrysosporium
ir
1.11.1.13
Mn2+ + H+ + H2O2
each catalytic cycle step is irreversible
439821
Phanerochaete chrysosporium
Mn3+ + H2O
chelation of Mn3+ by organic acids stabilizes Mn3+ at a high redox potential
439821
Phanerochaete chrysosporium
ir
1.11.1.13
Mn2+ + H+ + H2O2
free divalent Mn is the substrate, not Mn2+-complexes
439821
Phanerochaete chrysosporium
Mn3+ + H2O
alpha-hydroxy acids, e.g. lactate, facilitate the dissociation of Mn3+ from enzyme
439821
Phanerochaete chrysosporium
ir
1.11.1.13
Mn2+ + H+ + H2O2
free divalent Mn is the substrate, not Mn2+-complexes
439821
Phanerochaete chrysosporium
Mn3+ + H2O
Mn3+ oxidizes phenolic lignin model compounds
439821
Phanerochaete chrysosporium
ir
1.11.1.13
Mn2+ + H+ + H2O2
free divalent Mn is the substrate, not Mn2+-complexes
439821
Phanerochaete chrysosporium
Mn3+ + H2O
Mn3+ oxidizes vanillyl alcohol
439821
Phanerochaete chrysosporium
ir
1.11.1.13
Mn2+ + H+ + H2O2
free divalent Mn is the substrate, not Mn2+-complexes
439821
Phanerochaete chrysosporium
Mn3+ + H2O
Mn3+ oxidizes lignin
439821
Phanerochaete chrysosporium
ir
1.11.1.13
Mn2+ + H+ + H2O2
free divalent Mn is the substrate, not Mn2+-complexes
439821
Phanerochaete chrysosporium
Mn3+ + H2O
Mn3+-organic acid complexes oxidize terminal phenolic substrates in a second-order reaction
439821
Phanerochaete chrysosporium
ir
1.11.1.13
Mn2+ + H+ + H2O2
free divalent Mn is the substrate, not Mn2+-complexes
439821
Phanerochaete chrysosporium
Mn3+ + H2O
Mn3+ oxidizes thiols
439821
Phanerochaete chrysosporium
ir
1.11.1.13
Mn2+ + H+ + H2O2
free divalent Mn is the substrate, not Mn2+-complexes
439821
Phanerochaete chrysosporium
Mn3+ + H2O
Mn3+ acts as obligatory redox coupler, oxidizing various phenols, dyes and amines
439821
Phanerochaete chrysosporium
ir
1.11.1.13
Mn2+ + H+ + H2O2
free divalent Mn is the substrate, not Mn2+-complexes
439821
Phanerochaete chrysosporium
Mn3+ + H2O
the diffusible product is Mn3+
439821
Phanerochaete chrysosporium
ir
1.11.1.13
Mn2+ + H+ + H2O2
free divalent Mn is the substrate, not Mn2+-complexes
439821
Phanerochaete chrysosporium
Mn3+ + H2O
Mn3+ oxidizes amines
439821
Phanerochaete chrysosporium
ir
1.11.1.13
Mn2+ + H+ + H2O2
free divalent Mn is the substrate, not Mn2+-complexes
439821
Phanerochaete chrysosporium
Mn3+ + H2O
chelation of Mn3+ by organic acids stabilizes Mn3+ at a high redox potential
439821
Phanerochaete chrysosporium
ir
1.11.1.13
Mn2+ + H+ + H2O2
important component of lignin degradation system
439821
Phanerochaete chrysosporium
Mn3+ + H2O
Mn3+ is stabilized by chelating agents, malonate is the most effective physiological chelator excreted by the fungus
439821
Phanerochaete chrysosporium
?
1.11.1.13
Mn2+ + H+ + H2O2
each catalytic cycle step is irreversible
439821
Phanerochaete chrysosporium OGC101
Mn3+ + H2O
alpha-hydroxy acids, e.g. lactate, facilitate the dissociation of Mn3+ from enzyme
439821
Phanerochaete chrysosporium OGC101
ir
1.11.1.13
Mn2+ + H+ + H2O2
each catalytic cycle step is irreversible
439821
Phanerochaete chrysosporium OGC101
Mn3+ + H2O
Mn3+ oxidizes phenolic lignin model compounds
439821
Phanerochaete chrysosporium OGC101
ir
1.11.1.13
additional information
catalytic cycle with oxidized intermediates MnP compound I and II
439821
Phanerochaete chrysosporium
?
-
-
-
-
1.11.1.13
additional information
large substrates have no ready access to the catalytic center
439821
Phanerochaete chrysosporium
?
-
-
-
-
1.11.1.13
additional information
enzyme oxidizes 2,6-dimethoxyphenol
439821
Phanerochaete chrysosporium
?
-
-
-
-
1.11.1.13
additional information
catalytic cycle with oxidized intermediates MnP compound I and II
439821
Phanerochaete chrysosporium OGC101
?
-
-
-
-
1.11.1.13
additional information
large substrates have no ready access to the catalytic center
439821
Phanerochaete chrysosporium OGC101
?
-
-
-
-
1.11.1.13
additional information
enzyme oxidizes 2,6-dimethoxyphenol
439821
Phanerochaete chrysosporium OGC101
?
-
-
-
-
Temperature Optimum [C] (protein specific)
EC Number
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
1.11.1.13
25
-
assay at
Phanerochaete chrysosporium