Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Hochman, A.; Shemesh, A.
    Purification and characterization of a catalase-peroxidase from the photosynthetic bacterium Rhodopseudomonas capsulata (1987), J. Biol. Chem., 262, 6871-6876.
    View publication on PubMed

General Stability

EC Number General Stability Organism
1.11.1.21 both the catalatic and the peroxidatic activities are similarly inactivated by exposure to photooxidative conditions. The enzyme is reduced by sodium dithionite Rhodobacter capsulatus

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.11.1.6 H2O2
-
 Rhodobacter capsulatus
1.11.1.6 hydroxylamine 50% inhibition for catalase at 0.002 mM, for peroxidase at 0.078 mM Rhodobacter capsulatus
1.11.1.6 KCN 50% inhibition of both catalase and peroxidase activities at 0.02 mM Rhodobacter capsulatus
1.11.1.6 NaN3 50% inhibition for catalase at 0.15 mM, for peroxidase at 0.73 mM Rhodobacter capsulatus
1.11.1.21 azide
-
 Rhodobacter capsulatus
1.11.1.21 cyanide bo th enzymic activities of catalase-peroxidase are equally inhibited by cyanide Rhodobacter capsulatus
1.11.1.21 H2O2 both the catalatic and the peroxidatic activities are similarly inactivated by treatment with 1 mM H2O2 Rhodobacter capsulatus
1.11.1.21 hydroxylamine
-
 Rhodobacter capsulatus
1.11.1.21 additional information p-chloromercuribenzoate at concentrations up to 100 mM does not inhibit the enzyme Rhodobacter capsulatus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.11.1.6 59000
-
 4 * 59000, SDS-PAGE Rhodobacter capsulatus
1.11.1.6 236000
-
 gel filtration Rhodobacter capsulatus
1.11.1.21 236000
-
 gel filtration Rhodobacter capsulatus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.11.1.6 H2O2 Rhodobacter capsulatus bifunctional catalase-peroxidase O2 + H2O
-
 ?

Organic Solvent Stability

EC Number Organic Solvent Comment Organism
1.11.1.21 chloroform both the catalatic and the peroxidatic activities are similarly inactivated by exposure to chloroform Rhodobacter capsulatus
1.11.1.21 Ethanol both the catalatic and the peroxidatic activities are similarly inactivated by exposure to ethanol Rhodobacter capsulatus

Organism

EC Number Organism UniProt Comment Textmining
1.11.1.6 Rhodobacter capsulatus
-
-
-
1.11.1.21 Rhodobacter capsulatus
-
-
-

Oxidation Stability

EC Number Oxidation Stability Organism
1.11.1.6 sensitive to photooxidation Rhodobacter capsulatus

Purification (Commentary)

EC Number Purification (Comment) Organism
1.11.1.6 to homogeneity, chromatography techniques Rhodobacter capsulatus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.11.1.6 cell culture
-
 Rhodobacter capsulatus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.11.1.6 3,3'-diaminobenzidine + H2O2
-
 Rhodobacter capsulatus ? + H2O
-
 ?
1.11.1.6 H2O2 bifunctional catalase-peroxidase Rhodobacter capsulatus O2 + H2O
-
 ?
1.11.1.6 o-dianisidine + H2O2
-
 Rhodobacter capsulatus ? + H2O
-
 ?
1.11.1.6 pyrogallol + H2O2
-
 Rhodobacter capsulatus ? + H2O
-
 ?
1.11.1.21 diaminobenzidine + H2O2
-
 Rhodobacter capsulatus oxidized diaminobenzidine + H2O
-
 ?
1.11.1.21 H2O2
-
 Rhodobacter capsulatus O2 + H2O
-
 ?
1.11.1.21 additional information no reaction is detected with guaiacol or with ascorbate Rhodobacter capsulatus ?
-
 ?
1.11.1.21 o-dianisidine + H2O2
-
 Rhodobacter capsulatus oxidized o-dianisidine + H2O
-
 ?
1.11.1.21 pyrogallol + H2O2
-
 Rhodobacter capsulatus ? + H2O
-
 ?

Subunits

EC Number Subunits Comment Organism
1.11.1.6 tetramer 4 * 59000, SDS-PAGE Rhodobacter capsulatus
1.11.1.21 homotetramer
-
 Rhodobacter capsulatus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.11.1.6 50
-
 loss of both catalase-peroxidase activities Rhodobacter capsulatus
1.11.1.21 50
-
 both the catalatic and the peroxidatic activities are similarly inactivated by heating to 50°C Rhodobacter capsulatus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.11.1.21 5 5.3 peroxidatic activity Rhodobacter capsulatus
1.11.1.21 6 6.5 catalatic activity Rhodobacter capsulatus

Cofactor

EC Number Cofactor Comment Organism Structure
1.11.1.6 heme 1-1.2 protoheme IX per tetramer Rhodobacter capsulatus
1.11.1.21 heme there are 1-1.2 molecules of protoheme IX per tetrameric molecule Rhodobacter capsulatus

pI Value

EC Number Organism Comment pI Value Maximum pI Value
1.11.1.21 Rhodobacter capsulatus isoelectric focusing
-
 4.5

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
1.11.1.21 0.00215
-
 catalatic activity, in 100 mM potassium phosphate, pH 6.3, at 30°C Rhodobacter capsulatus hydroxylamine
1.11.1.21 0.022
-
 catalatic activity, in 100 mM potassium phosphate, pH 6.3, at 30°C Rhodobacter capsulatus cyanide
1.11.1.21 0.023
-
 peroxidatic activity, in 100 mM potassium phosphate, pH 5.2, at 30°C Rhodobacter capsulatus cyanide
1.11.1.21 0.078
-
 peroxidatic activity, in 100 mM potassium phosphate, pH 5.2, at 30°C Rhodobacter capsulatus hydroxylamine
1.11.1.21 0.15
-
 catalatic activity, in 100 mM potassium phosphate, pH 6.3, at 30°C Rhodobacter capsulatus azide
1.11.1.21 0.73
-
 peroxidatic activity, in 100 mM potassium phosphate, pH 5.2, at 30°C Rhodobacter capsulatus azide